LOCUS       sequence-311667-       10571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To detect the calcium ion releases from TRPV4 channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Li M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu 
            Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu
            YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou 
            Z, Xie XS, Hardie DG, Lin SC
  TITLE     Transient Receptor Potential V Channels Are Essential for Glucose 
            Sensing by Aldolase and AMPK.
  JOURNAL   Cell Metab. 2019 Sep 3;30(3):508-524.e12. doi: 
            10.1016/j.cmet.2019.05.018. Epub 2019 Jun 13.
  PUBMED    31204282
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2019.05.018"; journalName: "Cell Metab"; date: 
            "2019-09-3- 3"; volume: "30"; issue: "3"; pages: "508-524.e12"
COMMENT     Sequence Label: pBoBi-hTRPV4-C-GC6s
FEATURES             Location/Qualifiers
     source          1..10571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     815..839
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3539..3547,3548..3562,3563..3565,3566..3598,3599..3601,
                     3602..3625,3626..3652,3653..3709,3710..3715,3716..3985,
                     3986..4003,4004..4438,4439..4444,4445..4885)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKT
                     GHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      3632..3641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3922..3943
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4049..4070)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4310..4329)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     intron          4972..5201
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5184..5203
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5378..5966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5431..5451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5849..5860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6373..6553
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6579..6596)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6585..6809
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6855..7283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6942..6961)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7152..7173
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7292..7312)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7297..7626
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7477..7612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7539..7558
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7674..7721
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7740..8114
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8244..8365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8281..8300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8335..8354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8414..8430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8414..8430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8427..8449)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8438..8454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8462..8468,8469..8486,8487..8492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8507..8528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8645..8662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8816..9404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8896..8915)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9575..10366,10367..10435))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10198..10217
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10436..10540)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
      841 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactg agctccttaa
      901 ggttaactct agagaattcg gatccatggc ggattccagc gaaggccccc gcgcggggcc
      961 cggggaggtg gctgagctcc ccggggatga gagtggcacc ccaggcgggg aggcttttcc
     1021 tctctcctcc ctggccaatc tgtttgaggg ggaggatggc tccctttcgc cctcaccggc
     1081 tgatgccagt cgccctgctg gcccaggcga tgggcgacca aatctgcgca tgaagttcca
     1141 gggcgccttc cgcaaggggg tgcccaaccc catcgatctg ctggagtcca ccctatatga
     1201 gtcctcggtg gtgcctgggc ccaagaaagc acccatggac tcactgtttg actacggcac
     1261 ctatcgtcac cactccagtg acaacaagag gtggaggaag aagatcatag agaagcagcc
     1321 acagagcccc aaagcccctg cccctcagcc gccccccatc ctcaaagtct tcaaccggcc
     1381 tatcctcttt gacatcgtgt cccggggctc cactgctgac ctggacgggc tgctcccatt
     1441 cttgctgacc cacaagaaac gcctaactga tgaggagttt cgagagccat ctacggggaa
     1501 gacctgcctg cccaaggcct tgctgaacct gagcaatggc cgcaacgaca ccatccctgt
     1561 gctgctggac atcgcggagc gcaccggcaa catgcgggag ttcattaact cgcccttccg
     1621 tgacatctac tatcgaggtc agacagccct gcacatcgcc attgagcgtc gctgcaaaca
     1681 ctacgtggaa cttctcgtgg cccagggagc tgatgtccac gcccaggccc gtgggcgctt
     1741 cttccagccc aaggatgagg ggggctactt ctactttggg gagctgcccc tgtcgctggc
     1801 tgcctgcacc aaccagcccc acattgtcaa ctacctgacg gagaaccccc acaagaaggc
     1861 ggacatgcgg cgccaggact cgcgaggcaa cacagtgctg catgcgctgg tggccattgc
     1921 tgacaacacc cgtgagaaca ccaagtttgt taccaagatg tacgacctgc tgctgctcaa
     1981 gtgtgcccgc ctcttccccg acagcaacct ggaggccgtg ctcaacaacg acggcctctc
     2041 gcccctcatg atggctgcca agacgggcaa gattgggatc tttcagcaca tcatccggcg
     2101 ggaggtgacg gatgaggaca cacggcacct gtcccgcaag ttcaaggact gggcctatgg
     2161 gccagtgtat tcctcgcttt atgacctctc ctccctggac acgtgtgggg aagaggcctc
     2221 cgtgctggag atcctggtgt acaacagcaa gattgagaac cgccacgaga tgctggctgt
     2281 ggagcccatc aatgaactgc tgcgggacaa gtggcgcaag ttcggggccg tctccttcta
     2341 catcaacgtg gtctcctacc tgtgtgccat ggtcatcttc actctcaccg cctactacca
     2401 gccgctggag ggcacaccgc cgtaccctta ccgcaccacg gtggactacc tgcggctggc
     2461 tggcgaggtc attacgctct tcactggggt cctgttcttc ttcaccaaca tcaaagactt
     2521 gttcatgaag aaatgccctg gagtgaattc tctcttcatt gatggctcct tccagctgct
     2581 ctacttcatc tactctgtcc tggtgatcgt ctcagcagcc ctctacctgg cagggatcga
     2641 ggcctacctg gccgtgatgg tctttgccct ggtcctgggc tggatgaatg ccctttactt
     2701 cacccgtggg ctgaagctga cggggaccta tagcatcatg atccagaaga ttctcttcaa
     2761 ggaccttttc cgattcctgc tcgtctactt gctcttcatg atcggctacg cttcagccct
     2821 ggtctccctc ctgaacccgt gtgccaacat gaaggtgtgc aatgaggacc agaccaactg
     2881 cacagtgccc acttacccct cgtgccgtga cagcgagacc ttcagcacct tcctcctgga
     2941 cctgtttaag ctgaccatcg gcatgggcga cctggagatg ctgagcagca ccaagtaccc
     3001 cgtggtcttc atcatcctgc tggtgaccta catcatcctc acctttgtgc tgctcctcaa
     3061 catgctcatt gccctcatgg gcgagacagt gggccaggtc tccaaggaga gcaagcacat
     3121 ctggaagctg cagtgggcca ccaccatcct ggacattgag cgctccttcc ccgtattcct
     3181 gaggaaggcc ttccgctctg gggagatggt caccgtgggc aagagctcgg acggcactcc
     3241 tgaccgcagg tggtgcttca gggtggatga ggtgaactgg tctcactgga accagaactt
     3301 gggcatcatc aacgaggacc cgggcaagaa tgagacctac cagtattatg gcttctcgca
     3361 taccgtgggc cgcctccgca gggatcgctg gtcctcggtg gtaccccgcg tggtggaact
     3421 gaacaagaac tcgaacccgg acgaggtggt ggtgcctctg gacagcatgg ggaacccccg
     3481 ctgcgatggc caccagcagg gttacccccg caagtggagg actgatgacg ccccgctcat
     3541 gggttctcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
     3601 ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat cacgtcgtaa
     3661 gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac tcgagaacgt
     3721 ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa
     3781 catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga
     3841 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tttcgaaaga
     3901 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3961 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga
     4021 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4081 caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa
     4141 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4201 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4261 gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa
     4321 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4381 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct
     4441 gccggaccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga
     4501 caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg
     4561 gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga
     4621 cggcacaatc gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga
     4681 cacggaagaa gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat
     4741 cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga
     4801 ggttgatgaa atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga
     4861 gtttgtacaa atgatgacag cgaagctcga gccatggaag cttgatatct aactgactga
     4921 accggtggta ccgaggaatt aattcgctgt ctgcgagggc cagctgttgg ggtgagtact
     4981 ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg
     5041 atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag
     5101 aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc tggccataca
     5161 cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
     5221 actgcaggtc gagcatgcat ctagggcggc caattccgcc gatcctggaa aaacatggag
     5281 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5341 aggaggagga ggtgggtttt ccagtcacac ctcagacaat caacctctgg attacaaaat
     5401 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5461 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5521 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5581 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5641 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5701 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5761 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5821 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5881 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5941 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg
     6001 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6061 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6121 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     6181 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     6241 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6301 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     6361 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6421 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6481 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6541 aaaatctcta gcagggccgc tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6601 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6661 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6721 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6781 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6841 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6901 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6961 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7021 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7081 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7141 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7201 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7261 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7321 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7441 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7561 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7621 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     7681 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     7741 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     7801 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     7861 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     7921 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     7981 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     8041 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     8101 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     8161 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     8221 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     8281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8341 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8401 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8461 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8521 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8581 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8641 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8701 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9001 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9241 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10561 cacctgacgt c
//
