LOCUS       sequence-31167-e         535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Johnson RC, Penzes P, Eipper BA, Mains RE
  TITLE     Isoforms of kalirin, a neuronal Dbl family member, generated through
            use of different 5'- and 3'-ends along with an internal 
            translational initiation site.
  JOURNAL   J Biol Chem. 2000 Jun 23. 275(25):19324-33.
  PUBMED    10777487
REFERENCE   2  (bases 1 to 535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jun 23. 275(25):19324-33."
COMMENT     Sequence Label: pEAK10-His-Myc-Kal12
FEATURES             Location/Qualifiers
     source          1..535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             41..70
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcttgccacc atggcccatc accatcacca tcacaacatg gagcaaaagc tcatttctga
       61 ggaagatctc aatggtggtg gtggtgggtc gacaggatct tttcggaatg atggtttgaa
      121 agcttctgat gtccttccta tcctaaagga gaaagtggcc tttgtgtctg ggggtcgtga
      181 taagcgaggt ggacccatcc tgaccttccc tgcccgaagc aatcatgaca gaataagaca
      241 ggaagaccta cggaaactcg tgacgtattt ggccagcgtg ccaagcgagg acgtttgcaa
      301 acgtggcttc accgtcatca tcgacatgcg gggctccaag tgggacctca tcaagcccct
      361 cctcaagacg ctgcaggaag cctttcccgc cgagatccat gtggccctca tcatcaagcc
      421 tgacaacttc tggcagaagc agaagaccaa ctttggcagc tccaaattca tctttgagac
      481 gagcatggtg tctgtggagg gcctcacgaa gctggtggac ccctcccagc tgaca
//
