LOCUS       sequence-311671-       11410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To detect the calcium ion releases close to the lysosomal surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Li M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu 
            Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu
            YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou 
            Z, Xie XS, Hardie DG, Lin SC
  TITLE     Transient Receptor Potential V Channels Are Essential for Glucose 
            Sensing by Aldolase and AMPK.
  JOURNAL   Cell Metab. 2019 Sep 3;30(3):508-524.e12. doi: 
            10.1016/j.cmet.2019.05.018. Epub 2019 Jun 13.
  PUBMED    31204282
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2019.05.018"; journalName: "Cell Metab"; date: 
            "2019-09-3- 3"; volume: "30"; issue: "3"; pages: "508-524.e12"
COMMENT     Sequence Label: pBoBi-hLAMP2-C-GC6s
FEATURES             Location/Qualifiers
     source          1..11410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(4375..4383,4384..4401,4402..4404,4405..4437,4438..4440,
                     4441..4464,4465..4491,4492..4548,4549..4554,4555..4824,
                     4825..4842,4843..5277,5278..5283,5284..5724)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4471..4480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4761..4782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4888..4909)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5149..5168)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     intron          5811..6040
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6023..6042
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6217..6805
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6270..6290)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6688..6699)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7212..7392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7418..7435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7424..7648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7694..8122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7781..7800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7991..8012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8131..8151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8136..8465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8316..8451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8378..8397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8513..8560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8579..8953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9083..9204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9120..9139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9174..9193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9253..9269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9253..9269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9266..9288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9277..9293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9301..9307,9308..9325,9326..9331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9346..9367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9484..9501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9655..10243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9735..9754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10414..11205,11206..11274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11037..11056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11275..11379)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatggtg tgcttccgcc tcttcccggt tccgggctca
     3181 gggctcgttc tggtctgcct agtcctggga gctgtgcggt cttatgcatt ggaacttaat
     3241 ttgacagatt cagaaaatgc cacttgcctt tatgcaaaat ggcagatgaa tttcacagta
     3301 cgctatgaaa ctacaaataa aacttataaa actgtaacca tttcagacca tggcactgtg
     3361 acatataatg gaagcatttg tggggatgat cagaatggtc ccaaaatagc agtgcagttc
     3421 ggacctggct tttcctggat tgcgaatttt accaaggcag catctactta ttcaattgac
     3481 agcgtctcat tttcctacaa cactggtgat aacacaacat ttcctgatgc tgaagataaa
     3541 ggaattctta ctgttgatga acttttggcc atcagaattc cattgaatga cctttttaga
     3601 tgcaatagtt tatcaacttt ggaaaagaat gatgttgtcc aacactactg ggatgttctt
     3661 gtacaagctt ttgtccaaaa tggcacagtg agcacaaatg agttcctgtg tgataaagac
     3721 aaaacttcaa cagtggcacc caccatacac accactgtgc catctcctac tacaacacct
     3781 actccaaagg aaaaaccaga agctggaacc tattcagtta ataatggcaa tgatacttgt
     3841 ctgctggcta ccatggggct gcagctgaac atcactcagg ataaggttgc ttcagttatt
     3901 aacatcaacc ccaatacaac tcactccaca ggcagctgcc gttctcacac tgctctactt
     3961 agactcaata gcagcaccat taagtatcta gactttgtct ttgctgtgaa aaatgaaaac
     4021 cgattttatc tgaaggaagt gaacatcagc atgtatttgg ttaatggctc cgttttcagc
     4081 attgcaaata acaatctcag ctactgggat gcccccctgg gaagttctta tatgtgcaac
     4141 aaagagcaga ctgtttcagt gtctggagca tttcagataa atacctttga tctaagggtt
     4201 cagcctttca atgtgacaca aggaaagtat tctacagccc aagagtgttc gctggatgat
     4261 gacaccattc taatcccaat tatagttggt gctggtcttt caggcttgat tatcgttata
     4321 gtgattgctt acgtaattgg cagaagaaaa agttatgctg gatatcagac tctgatgggt
     4381 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg
     4441 gatctgtacg acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag
     4501 tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtc
     4561 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttccacat ccgccacaac
     4621 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac
     4681 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac
     4741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4801 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag
     4861 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4921 aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag
     4981 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5041 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5101 tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac
     5161 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5221 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacctg
     5281 ccggaccaac tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac
     5341 aaggacgggg atgggacaat aacaaccaag gagctgggga cggtgatgcg gtctctgggg
     5401 cagaacccca cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac
     5461 ggcacaatcg acttccctga gttcctgaca atgatggcaa gaaaaatgaa atacagggac
     5521 acggaagaag aaattagaga agcgttcggt gtgtttgata aggatggcaa tggctacatc
     5581 agtgcagcag agcttcgcca cgtgatgaca aaccttggag agaagttaac agatgaagag
     5641 gttgatgaaa tgatcaggga agcagacatc gatggggatg gtcaggtaaa ctacgaagag
     5701 tttgtacaaa tgatgacagc gaagctcgag ccatggaagc ttgatatcta actgactgaa
     5761 ccggtggtac cgaggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
     5821 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     5881 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
     5941 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
     6001 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     6061 ctgcaggtcg agcatgcatc tagggcggcc aattccgccg atcctggaaa aacatggagc
     6121 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     6181 ggaggaggag gtgggttttc cagtcacacc tcagacaatc aacctctgga ttacaaaatt
     6241 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6301 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6361 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6421 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6481 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6541 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6601 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6661 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6721 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6781 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     6841 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6901 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6961 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7021 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7081 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7141 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7201 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7261 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7321 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7381 aaatctctag cagggccgct ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7441 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7501 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7561 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7621 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7681 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8101 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8461 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     8521 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     8581 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     8641 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     8701 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     8761 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     8821 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     8881 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     8941 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9001 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9061 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9181 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11401 acctgacgtc
//
