LOCUS       sequence-311682-        3081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pKO2.1
FEATURES             Location/Qualifiers
     source          1..3081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             262..834
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          911..976
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1037..1171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1074..1093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1128..1147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1191..1207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1262..1271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1315..1419
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1420..1488,1489..2280)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1638..1657)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2451..3039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2940..2959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggaccgaac aactccgccg cgcgcgctcc gtgtaggccg gtgcgggcgg ccccgtagcg
       61 caagggaggg cgggaaagga aggggcggga cacaagggcg aatctataaa gggcgtcact
      121 cagccagttc tctcctcaga agcgccgaga gcgcgaccgg gacggttgga gaagaaggtg
      181 gctcccggaa gggggagaga caaactgccg taacctctgc cgttcaggat ctgcgacctg
      241 caggtcctcg ccatggaccc tgatgatgtt gttgattctt ctaaatcttt tgtgatggaa
      301 aacttttctt cgtaccacgg gactaaacct ggttatgtag attccattca aaaaggtata
      361 caaaagccaa aatctggtac acaaggaaat tatgacgatg attggaaagg gttttatagt
      421 accgacaata aatacgacgc tgcgggatac tctgtagata atgaaaaccc gctctctgga
      481 aaagctggag gcgtggtcaa agtgacgtat ccaggactga cgaaggttct cgcactaaaa
      541 gtggataatg ccgaaactat taagaaagag ttaggtttaa gtctcactga accgttgatg
      601 gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg atggtgcttc gcgtgtagtg
      661 ctcagccttc ccttcgctga ggggagttct agcgttgaat atattaataa ctgggaacag
      721 gcgaaagcgt taagcgtaga acttgagatt aattttgaaa cccgtggaaa acgtggccaa
      781 gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag gcgatctctt
      841 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
      901 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
      961 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     1021 agatccacta ggatctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1141 actcatcaat gtatcttatc atgtctggat cgtagttcta gagcggaccg gtaaaacgac
     1201 ggccagtgtt aactctagga attctctagg gtacctctag gagatcttct agctcgacgc
     1261 ggccgccatg gtcatagctg tttgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcac gagacaataa
     1381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2101 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2581 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3061 ttacggttct ggccttttgc a
//
