LOCUS       sequence-311686-       14361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pSLIK 3xFLAG-MAVS hygro
FEATURES             Location/Qualifiers
     source          1..14361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    196..313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    370..394
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        426..740
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    470..488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    505..523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    541..559
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    577..595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    612..630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    648..666
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     713..737
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             749..814
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(2479..2503)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2553..3764
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3601..3620
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3889..4506,4507..4512,4513..4548,4549..4551,4552..4587,
                     4588..4590,4591..4626,4627..4635)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4688..5261
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4855..4872)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5082..5101
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5320..6339
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5320..6339
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6805..7393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6858..6878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7276..7287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7396..7412)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7397..7413)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7922..8102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8128..8145)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8134..8358
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8404..8832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8491..8510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8701..8722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8841..8861)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8846..9175
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9026..9161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9088..9107
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9223..9270
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9289..9663
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9793..9914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9830..9849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9884..9903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9963..9979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9963..9979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9976..9998)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9987..10003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10011..10017,10018..10035,10036..10041))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10056..10077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10194..10211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10365..10953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10445..10464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11124..11915,11916..11984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11747..11766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11985..12089)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12164..12183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12355..12734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12736..12934
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12952..13132
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13179..13304
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13797..14030
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14215..14259
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
       61 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
      121 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
      181 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      241 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      301 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattctaga
      361 cagttatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
      421 atcccgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      481 gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      541 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      601 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      661 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      721 tagtgaaccg tcagatcgcc actagatgga ctacaaagac catgacggtg attataaaga
      781 tcatgacatc gattacaagg atgacgatga caagactagt atgccgtttg ctgaagacaa
      841 gacctataag tatatctgcc gcaatttcag caatttttgc aatgtggatg ttgtagagat
      901 tctgccttac ctgccctgcc tcacagcaag agaccaggat cgactgcggg ccacctgcac
      961 actctcaggg aaccgggaca ccctctggca tctcttcaat acccttcagc ggcggcccgg
     1021 ctgggtggag tacttcattg cggcactgag gggctgtgag ctagttgatc tcgcggacga
     1081 agtggcctct gtctacgaga gctaccagcc tcggacctcg gaccgtcccc cagacccact
     1141 ggagccaccg tcacttcctg ctgagaggcc agggcccccc acacctgctg cggcccacag
     1201 catcccctac aacagctgca gagagaagga gccaagttac cccatgcctg tccaggagac
     1261 ccaggcgcca gagtccccag gagagaattc agagcaagcc ctgcagacgc tcagccccag
     1321 agccatccca aggaatccag atggtggccc cctggagtcc tcctctgacc tggcagccct
     1381 cagccctctg acctccagcg ggcatcagga gaaggacaca gaactgggca gtacccacac
     1441 agcaggtgcg acctccagcc tcacaccatc ccgtgggcct gtgtctccat ctgtctcctt
     1501 ccagcccctg gcccgttcca cccccagggc aagccgcttg cctggaccca cagggtcagt
     1561 tgtatctact ggcacctcct tctcctcctc atcccctggc ttggcctctg caggggctgc
     1621 agagggtaaa cagggtgcag agagtgacca ggccgagcct atcatctgct ccagtggggc
     1681 agaggcacct gccaactctc tgccctccaa agtgcctacc accttgatgc ctgtgaacac
     1741 agtggccctg aaagtgcctg ccaacccagc atctgtcagc acagtgccct ccaagttgcc
     1801 aactagctca aagccccctg gtgcagtgcc ttctaatgcg ctcaccaatc cagcaccatc
     1861 caaattgccc atcaactcaa cccgtgctgg catggtgcca tccaaagtgc ctactagcat
     1921 ggtgctcacc aaggtgtctg ccagcacagt ccccactgac gggagcagca gaaatgagga
     1981 gaccccagca gctccaacac ccgccggcgc cactggaggc agctcagcct ggctagacag
     2041 cagctttgag aataggggcc ttgggtcgga gctgagtaag cctggcgtgc tggcatccca
     2101 ggtagacagc ccgttctcgg gctgcttcga ggatcttgcc atcagtgcca gcacctcctt
     2161 gggcatgggg ccctgccatg gcccagagga gaatgagtat aagtccgagg gcacctttgg
     2221 gatccacgtg gctgagaacc ccagcatcca gctcctggag ggcaaccctg ggccacctgc
     2281 ggacccggat ggcggcccca ggccacaagc cgaccggaag ttccaggaga gggaggtgcc
     2341 atgccacagg ccctcacctg gggctctgtg gctccaggtg gctgtgacag gggtgctggt
     2401 agtcacactc ctggtggtgc tgtaccggcg gcgtctgcac tagcaattgg aagactaatg
     2461 cgttttcgag atatctagac ccagctttct tgtacaaagt ggttgataac atctcgagcc
     2521 ataaagatgg ttaattaacc cacccaagat ctggcctccg cgccgggttt tggcgcctcc
     2581 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
     2641 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     2701 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     2761 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     2821 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     2881 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     2941 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     3001 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     3061 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
     3121 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     3181 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     3241 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     3301 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
     3361 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     3421 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     3481 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     3541 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     3601 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     3661 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     3721 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct tggtaccgag
     3781 ctcggatctc caccccgtac cggtcctgca ggatttaaat ggcgcgccgc tagcgctacc
     3841 ggactcagat ctcgagctca agcttcgaat tctgcagtcg aattcaccat gtctagactg
     3901 gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc
     3961 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     4021 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     4081 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     4141 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     4201 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     4261 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     4321 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     4381 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     4441 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     4501 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     4561 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     4621 atgctccccg ggtaactaag taaggatccg cggccgcact agaggaattc cgcccctctc
     4681 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     4741 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     4801 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     4861 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     4921 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     4981 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5041 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5101 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5161 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5221 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttaccgg tacgcgtgat
     5281 agcttgccac aacccgtacc aaagatggat agatccggaa agcctgaact caccgcgacg
     5341 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5401 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5461 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5521 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     5581 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     5641 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     5701 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     5761 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     5821 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     5881 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     5941 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6001 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6061 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6121 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6181 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6241 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6301 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag
     6361 agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag
     6421 agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct
     6481 gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt ggtccccagg
     6541 gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat tggagatcgc
     6601 cgcccgtgcc tgccgattgg gtgcagatct ctagatctgt acaagtaaag cggccgcgac
     6661 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     6721 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc
     6781 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     6841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7201 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7381 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
     7441 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     7501 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     7561 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7621 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7681 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     7741 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     7801 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     7861 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     7921 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7981 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8041 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8101 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     8161 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     8221 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     8281 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     8341 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     8401 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8461 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8521 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8581 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8641 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8701 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8761 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8821 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8881 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8941 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9001 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     9121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9181 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     9241 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     9301 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     9361 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     9421 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     9481 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     9541 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     9601 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     9661 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9721 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9781 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9901 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9961 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10021 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10081 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10141 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10801 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11401 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12121 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    12181 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    12241 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    12301 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    12361 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    12421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    12481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    12541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    12601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    12661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    12721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
    12781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
    12841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
    12901 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
    12961 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    13021 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    13081 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    13141 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    13201 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    13261 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    13321 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    13381 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    13441 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    13501 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    13561 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    13621 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
    13681 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    13741 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    13801 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    13861 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    13921 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    13981 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    14041 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    14101 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    14161 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    14221 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    14281 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    14341 atagtttttg ctgtactttc t
//
