LOCUS       sequence-31172-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Milgram SL, Johnson RC, Mains RE
  TITLE     Expression of individual forms of peptidylglycine alpha-amidating 
            monooxygenase in AtT-20 cells: endoproteolytic processing and 
            routing to secretory granules.
  JOURNAL   J Cell Biol. 1992 May . 117(4):717-28.
  PUBMED    1577852
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1992 May . 117(4):717-28."
COMMENT     Sequence Label: pCIS-krPAM2
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     271..290
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      339..348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
      121 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
      181 ttggcttctt atgcgacgga tcccgtaata agcttgaggt gtggcaggct tgagatctgg
      241 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
      301 aggtccaact gcagctcggt tctatcgata agcttgccgc caccatggcc ggacgcgccc
      361 gcagcggtct gctactgctg ctgctggggc tgctcgccct gcagagcagc tgcctggcct
      421 tcagaagccc actttctgtc tttaagaggt ttaaagaaac taccagatca ttttccaatg
      481 aatgccttgg taccattgga ccagtcaccc ctcttgatgc atcagatttt gcgctggata
      541 ttcgcatgcc tggggttaca cctaaagagt ctgacacata cttctgcatg tccatgcgtc
      601 tgcctgtgga tgaggaagcc ttcgtgattg acttcaagcc tcgtgccagc atggatactg
      661 tccaccatat gctgctgttt ggatgcaata tgccctcgtc cactggaagt tactggtttt
      721 gtgatgaagg aacctgtaca gataaagcca atattctata tgcctgggca aggaatgctc
      781 cccccacccg gctcccgaaa ggtgttggat tcagagttgg aggagaaact ggaagcaaat
      841 acttcgtcct tcaagttcac tatggcgata tcagtgcttt tcgagataat cacaaagact
      901 gctctggcgt gtccgtacat ctcacacgtg t
//
