LOCUS       sequence-311722-       11251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted simultaneous C-to-T and A-to-G in rice protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11251)
  TITLE     Gao Lab Plasmids
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STEME-2
FEATURES             Location/Qualifiers
     source          1..11251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3856..3879,3880..3882,3883..7956)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7869..7877
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7957..8004
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8023..8271
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8284..8304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8362..8538
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9092..9108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9092..9108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9105..9127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9116..9132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9140..9146,9147..9164,9165..9170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9185..9206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9323..9340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9494..10082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9574..9593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10253..11044,11045..11113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10876..10895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11114..11218)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caagctaatt cgagctcggt acctgacccg gtcgtgcccc tctctagaga taatgagcat
       61 tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc
      121 agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt
      181 actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa
      241 ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt
      301 tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca
      361 tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt
      421 attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat
      481 aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag
      541 aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa
      601 acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa
      661 gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga tcgagagttc
      721 cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag
      781 acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg
      841 attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc
      901 cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat
      961 ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc
     1021 cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct
     1081 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg
     1141 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct
     1201 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt
     1261 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac
     1321 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg
     1381 ttgggcggtc gttctagatc ggagtagaat taattctgtt tcaaactacc tggtggattt
     1441 attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat
     1501 ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat
     1561 acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat
     1621 tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga
     1681 actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga
     1741 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg
     1801 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt
     1861 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga
     1921 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg
     1981 ctcaccctgt agtttggtgt tacttctgca aagcttatgt ccgaggtgga gttctctcac
     2041 gagtattgga tgaggcacgc tcttacactt gctaagagag cttgggacga aagagaagtg
     2101 ccagttggcg ccgttcttgt gcataataat agggtgatcg gcgagggttg gaatagacca
     2161 attggaaggc atgatccaac agctcacgca gagattatgg ctctcagaca aggcggcctc
     2221 gttatgcaga actacaggct cattgacgct acactctacg tgacactcga accttgcgtt
     2281 atgtgcgccg gagctatgat tcattctagg attggcaggg tcgtgtttgg agctagggac
     2341 gctaaaacag gagccgccgg atctcttatg gacgtgttgc atcatccagg catgaaccat
     2401 agggtggaga ttacagaggg cattcttgca gacgagtgcg ctgctcttct ttccgatttc
     2461 ttcaggatga gaaggcagga gattaaggcc cagaagaagg ctcagtcttc tacagatagc
     2521 ggaggatctt ccggaggatc tagcggctcc gagacaccag gaacatccga aagcgctaca
     2581 ccagaatcta gcggaggctc ttccggagga tcttctgaag tggagttctc ccacgagtat
     2641 tggatgaggc acgctcttac acttgctaaa agggctaggg acgaaaggga agttccagtt
     2701 ggagctgttc tcgtgctcaa taacagggtg attggcgagg gttggaatag agccattgga
     2761 ctccatgatc caacagctca cgcagagatt atggctctta gacaaggcgg cctcgttatg
     2821 cagaattaca gactcatcga cgccacactc tacgttacct tcgaaccttg cgttatgtgc
     2881 gccggagcta tgatccattc taggattggc agggtcgtgt tcggcgttag aaacgctaag
     2941 acaggagctg caggctctct tatggacgtt cttcattacc caggcatgaa tcatagagtg
     3001 gagatcacag aaggcattct tgcagacgag tgcgcagctc tcctttgcta tttcttcagg
     3061 atgccgaggc aagttttcaa cgctcagaag aaggcccagt cttctacaga ttccggcgga
     3121 tcttctggag gatctagcgg ctccgagaca ccaggaacat ccgaatccgc tacaccagag
     3181 tcttctggag gatctagcgg aggatctgag gccagcccgg ctagcggccc aaggcatctc
     3241 atggacccgc acatcttcac cagcaacttc aacaacggca tcggcaggca caagacctac
     3301 ttgtgctacg aggtggagag gctcgacaac ggaacctccg tgaagatgga ccaacacagg
     3361 gggttcctcc acaaccaagc caagaacctc ctctgcggct tctacggcag gcacgccgag
     3421 ttgaggttcc tcgacttggt gccatccctc caactcgatc cagcccaaat ctaccgcgtg
     3481 acctggttca tctcctggtc cccatgcttc tcctggggtt gcgccggcga ggttcgggct
     3541 ttcctccaag aaaacaccca cgtccgcctc cgcattttcg ccgccaggat ctatgattac
     3601 gaccctctct acaaggaggc cctccagatg ctgcgggacg ccggtgctca ggtgagtatc
     3661 atgacctacg acgagttcaa gcactgctgg gacaccttcg ttgaccacca gggctgccca
     3721 ttccaaccat gggacggtct ggatgaacac agccaagcct tgtccggcag gctccgggcc
     3781 atcctccaaa accaggggaa ctccgggagc gagacgccag gcacctccga gtcggccacc
     3841 ccagaatctc ttaaggacaa gaagtactcg atcggcctcg ccattgggac taactctgtt
     3901 ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc
     3961 aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt cgacagcggc
     4021 gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac gcgcaggaag
     4081 aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt tgacgattct
     4141 ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca cgagcggcat
     4201 ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc tacgatctac
     4261 catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct gatctacctc
     4321 gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga tctcaacccg
     4381 gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa ccagctcttc
     4441 gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc
     4501 tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa gaagaacggc
     4561 ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc
     4621 gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga tgacctggat
     4681 aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc caagaatctg
     4741 tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac gaaggctccg
     4801 ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag
     4861 gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga tcagtcgaag
     4921 aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta caagttcatc
     4981 aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct caatcgcgag
     5041 gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca gattcatctc
     5101 ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct caaggataac
     5161 cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg cccactcgct
     5221 aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat cacgccgtgg
     5281 aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga gaggatgacg
     5341 aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct cctgtacgag
     5401 tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg catgaggaag
     5461 ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt caagaccaac
     5521 cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga gtgcttcgat
     5581 tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac ctaccacgat
     5641 ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga ggatatcctc
     5701 gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga ggagcgcctg
     5761 aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag gaggaggtac
     5821 accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa gcagtccggg
     5881 aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt catgcagctg
     5941 attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt gagcggccag
     6001 ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat caagaagggg
     6061 attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag gcacaagcct
     6121 gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg ccagaagaac
     6181 tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc ccagatcctc
     6241 aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct gtactacctc
     6301 cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct cagcgattac
     6361 gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga caacaaggtc
     6421 ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga ggaggtcgtt
     6481 aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac gcagcggaag
     6541 ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa ggcgggcttc
     6601 atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca gattctcgac
     6661 tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt gaaggtcatc
     6721 accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgg
     6781 gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt cggcacagct
     6841 ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta caaggtttac
     6901 gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac cgccaagtac
     6961 ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc caatggcgag
     7021 atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt gtgggacaag
     7081 ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag
     7141 aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa gcggaacagc
     7201 gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg gttcgacagc
     7261 cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag
     7321 ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag cttcgagaag
     7381 aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga cctgatcatt
     7441 aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat gctggcttcc
     7501 gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt gaacttcctc
     7561 tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga gcagaagcag
     7621 ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat ttccgagttc
     7681 tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc gtacaacaag
     7741 caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt caccctgacg
     7801 aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg caagaggtac
     7861 acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac cggcctctac
     7921 gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc gacgaagaag
     7981 gcggggcagg cgaagaagaa gaaggaattc tccggcggca gcaccaacct gtccgacatc
     8041 atcgagaagg agacgggcaa gcaactcgtg atccaggaga gcatcctcat gctgccagag
     8101 gaggtggagg aggtcatcgg caacaagcca gagtccgaca tcctggtgca caccgcctac
     8161 gacgagtcca ccgacgagaa cgtcatgctc ctgaccagcg acgccccaga gtacaagcca
     8221 tgggccctcg tcatccagga cagcaacggg gagaacaaga tcaagatgct gtcggggggg
     8281 agcccaaaga agaagcggaa ggtgtaggaa ttcggtacgc tgaaatcacc agtctctctc
     8341 tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg
     8401 gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     8461 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     8521 agtactaaaa tccagatctc ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga
     8581 cacgagacga ctaaacctgg agcccagacg ccgttcgaag ctagaagtac cgcttaggca
     8641 ggaggccgtt agggaaaaga tgctaaggca gggttggtta cgttgactcc cccgtaggtt
     8701 tggtttaaat atgatgaagt ggacggaagg aaggaggaag acaaggaagg ataaggttgc
     8761 aggccctgtg caaggtaaga agatggaaat ttgatagagg tacgctacta tacttatact
     8821 atacgctaag ggaatgcttg tatttatacc ctataccccc taataacccc ttatcaattt
     8881 aagaaataat ccgcataagc ccccgcttaa aaattggtat cagagccata aataggtcta
     8941 tgaccaaaac tcaagaggat aaaacctcac caaaatacga aagagttctt aactctaaag
     9001 ataaaagatc tttcaagatc aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg
     9061 atatctcgag atctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaagaatctc
    10081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11221 catttccccg aaaagtgcca cctgccagtg c
//
