LOCUS       sequence-311725-       11719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted simultaneous C-to-T and A-to-G in rice protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11719)
  TITLE     Gao Lab Plasmids
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STEME-3
FEATURES             Location/Qualifiers
     source          1..11719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3946..3969,3970..3972,3973..8046)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7959..7967
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8047..8094
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8101..8154
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8155..8403
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8416..8436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8437..8490
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8491..8739
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8752..8772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8830..9006
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9560..9576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9560..9576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9573..9595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9584..9600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9608..9614,9615..9632,9633..9638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9653..9674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9791..9808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9962..10550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10042..10061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10721..11512,11513..11581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11344..11363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11582..11686)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caagctaatt cgagctcggt acctgacccg gtcgtgcccc tctctagaga taatgagcat
       61 tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc
      121 agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt
      181 actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa
      241 ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt
      301 tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca
      361 tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt
      421 attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat
      481 aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag
      541 aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa
      601 acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa
      661 gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga tcgagagttc
      721 cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag
      781 acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg
      841 attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc
      901 cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat
      961 ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc
     1021 cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct
     1081 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg
     1141 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct
     1201 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt
     1261 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac
     1321 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg
     1381 ttgggcggtc gttctagatc ggagtagaat taattctgtt tcaaactacc tggtggattt
     1441 attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat
     1501 ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat
     1561 acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat
     1621 tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga
     1681 actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga
     1741 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg
     1801 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt
     1861 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga
     1921 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg
     1981 ctcaccctgt tgtttggtgt tacttctgca aagcttatgg aggccagccc ggctagcggc
     2041 ccaaggcatc tcatggaccc gcacatcttc accagcaact tcaacaacgg catcggcagg
     2101 cacaagacct acttgtgcta cgaggtggag aggctcgaca acggaacctc cgtgaagatg
     2161 gaccaacaca gggggttcct ccacaaccaa gccaagaacc tcctctgcgg cttctacggc
     2221 aggcacgccg agttgaggtt cctcgacttg gtgccatccc tccaactcga tccagcccaa
     2281 atctaccgcg tgacctggtt catctcctgg tccccatgct tctcctgggg ttgcgccggc
     2341 gaggttcggg ctttcctcca agaaaacacc cacgtccgcc tccgcatttt cgccgccagg
     2401 atctatgatt acgaccctct ctacaaggag gccctccaga tgctgcggga cgccggtgct
     2461 caggtgagta tcatgaccta cgacgagttc aagcactgct gggacacctt cgttgaccac
     2521 cagggctgcc cattccaacc atgggacggt ctggatgaac acagccaagc cttgtccggc
     2581 aggctccggg ccatcctcca aaaccagggg aactccggga gcgagacgcc aggcacctcc
     2641 gagtcggcca ccccagaatc ttccggcgga tcttctggag gatctagcgg ctccgagaca
     2701 ccaggaacat ccgaatccgc tacaccagag tcttctggag gatctagcgg aggatcttcc
     2761 gaggtggagt tctctcacga gtattggatg aggcacgctc ttacacttgc taagagagct
     2821 tgggacgaaa gagaagtgcc agttggcgcc gttcttgtgc ataataatag ggtgatcggc
     2881 gagggttgga atagaccaat tggaaggcat gatccaacag ctcacgcaga gattatggct
     2941 ctcagacaag gcggcctcgt tatgcagaac tacaggctca ttgacgctac actctacgtg
     3001 acactcgaac cttgcgttat gtgcgccgga gctatgattc attctaggat tggcagggtc
     3061 gtgtttggag ctagggacgc taaaacagga gccgccggat ctcttatgga cgtgttgcat
     3121 catccaggca tgaaccatag ggtggagatt acagagggca ttcttgcaga cgagtgcgct
     3181 gctcttcttt ccgatttctt caggatgaga aggcaggaga ttaaggccca gaagaaggct
     3241 cagtcttcta cagatagcgg aggatcttcc ggaggatcta gcggctccga gacaccagga
     3301 acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc ttctgaagtg
     3361 gagttctccc acgagtattg gatgaggcac gctcttacac ttgctaaaag ggctagggac
     3421 gaaagggaag ttccagttgg agctgttctc gtgctcaata acagggtgat tggcgagggt
     3481 tggaatagag ccattggact ccatgatcca acagctcacg cagagattat ggctcttaga
     3541 caaggcggcc tcgttatgca gaattacaga ctcatcgacg ccacactcta cgttaccttc
     3601 gaaccttgcg ttatgtgcgc cggagctatg atccattcta ggattggcag ggtcgtgttc
     3661 ggcgttagaa acgctaagac aggagctgca ggctctctta tggacgttct tcattaccca
     3721 ggcatgaatc atagagtgga gatcacagaa ggcattcttg cagacgagtg cgcagctctc
     3781 ctttgctatt tcttcaggat gccgaggcaa gttttcaacg ctcagaagaa ggcccagtct
     3841 tctacagatt ccggcggatc ttctggagga tctagcggct ccgagacacc aggaacatcc
     3901 gaatccgcta caccagagtc ttctggagga tctagcggag gatctgacaa gaagtactcg
     3961 atcggcctcg ccattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag
     4021 gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat
     4081 ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc
     4141 accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc
     4201 tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc
     4261 gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt
     4321 gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca
     4381 gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc
     4441 catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc
     4501 cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc
     4561 gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc
     4621 gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg
     4681 gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc
     4741 tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac
     4801 gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc
     4861 agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac
     4921 gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag
     4981 tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg
     5041 gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg
     5101 gaggagctgc tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat
     5161 aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag
     5221 gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc
     5281 aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc
     5341 cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct
     5401 agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag
     5461 gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag
     5521 gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag
     5581 gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag
     5641 gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc
     5701 ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc
     5761 ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc
     5821 gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag
     5881 gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc
     5941 atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat
     6001 ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag
     6061 gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac
     6121 ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag
     6181 ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag
     6241 aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag
     6301 ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg
     6361 cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag
     6421 gagctggata ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc
     6481 ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag
     6541 tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc
     6601 ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc
     6661 gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag
     6721 atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat
     6781 gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc
     6841 aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac
     6901 gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc
     6961 gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag
     7021 caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc
     7081 aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac
     7141 ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt
     7201 ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct
     7261 aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg
     7321 gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg
     7381 gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc
     7441 acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc
     7501 tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg
     7561 gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg
     7621 gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag
     7681 ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc
     7741 gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat
     7801 ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct
     7861 gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac
     7921 ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc
     7981 ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg
     8041 ggcgacaagc ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaaggaattc
     8101 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctaccaac
     8161 ctgtccgaca tcatcgagaa ggagacgggc aagcaactcg tgatccagga gagcatcctc
     8221 atgctgccag aggaggtgga ggaggtcatc ggcaacaagc cagagtccga catcctggtg
     8281 cacaccgcct acgacgagtc caccgacgag aacgtcatgc tcctgaccag cgacgcccca
     8341 gagtacaagc catgggccct cgtcatccag gacagcaacg gggagaacaa gatcaagatg
     8401 ctgtcggggg ggagcccaaa gaagaagcgg aaggtggagg gcagaggaag tcttctaaca
     8461 tgcggtgacg tggaggagaa tcccggccct accaacctgt ccgacatcat cgagaaggag
     8521 acgggcaagc aactcgtgat ccaggagagc atcctcatgc tgccagagga ggtggaggag
     8581 gtcatcggca acaagccaga gtccgacatc ctggtgcaca ccgcctacga cgagtccacc
     8641 gacgagaacg tcatgctcct gaccagcgac gccccagagt acaagccatg ggccctcgtc
     8701 atccaggaca gcaacgggga gaacaagatc aagatgctgt cgggggggag cccaaagaag
     8761 aagcggaagg tgtagaccgg tggtacgctg aaatcaccag tctctctcta caaatctatc
     8821 tctctctatt ttctccataa ataatgtgtg agtagtttcc cgataaggga aattagggtt
     8881 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     8941 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     9001 cagatctcct aaagtcccta tagatctttg tcgtgaatat aaaccagaca cgagacgact
     9061 aaacctggag cccagacgcc gttcgaagct agaagtaccg cttaggcagg aggccgttag
     9121 ggaaaagatg ctaaggcagg gttggttacg ttgactcccc cgtaggtttg gtttaaatat
     9181 gatgaagtgg acggaaggaa ggaggaagac aaggaaggat aaggttgcag gccctgtgca
     9241 aggtaagaag atggaaattt gatagaggta cgctactata cttatactat acgctaaggg
     9301 aatgcttgta tttataccct atacccccta ataacccctt atcaatttaa gaaataatcc
     9361 gcataagccc ccgcttaaaa attggtatca gagccatgaa taggtctatg accaaaactc
     9421 aagaggataa aacctcacca aaatacgaaa gagttcttaa ctctaaagat aaaagatctt
     9481 tcaagatcaa aactagttcc ctcacaccgg tgacggggat cgcatgcgat atctcgagat
     9541 ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9721 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11701 aagtgccacc tgccagtgc
//
