LOCUS       sequence-311742-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bao L, Menon PNK, Liljeruhm J, Forster AC
  TITLE     Overcoming chromoprotein limitations by engineering a red 
            fluorescent protein.
  JOURNAL   Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936.
  PUBMED    32891596
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936."
COMMENT     Sequence Label: pSB1C3-J23110-B0034-mRFP1E
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        23..57
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             84..761
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    790..810
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacaa tgctagctac
       61 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      121 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      181 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      241 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      301 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      361 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      421 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      481 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      541 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
      601 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      661 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      721 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
      781 gtagatcgct actagtagcg gccgctgcag
//
