LOCUS       sequence-31176-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Chufan EE, De M, Eipper BA, Mains RE, Amzel LM
  TITLE     Amidation of bioactive peptides: the structure of the lyase domain 
            of the amidating enzyme.
  JOURNAL   Structure. 2009 Jul 15. 17(7):965-73.
  PUBMED    19604476
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2009 Jul 15. 17(7):965-73."
COMMENT     Sequence Label: pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          169..301
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     284..303
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        346..364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             468..485
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca
       61 gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagtg
      121 actctcttaa ggtagccttg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      181 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      241 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      301 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      361 taggctagat cgataagctt gccgccacca tggccggacg cgcccgcagc ggtctgctac
      421 tgctgctgct ggggctgctc gccctgcaga gcagctgcct ggccgcccac catcaccatc
      481 accatgctag cgatttccat gtggaagaag aactggactg gcctggagtg tacttgttac
      541 caggccaggt ttctggggtg gccctggatt ctaagaataa cctagtgatt ttccacagag
      601 gtgaccatgt ttgggatgga aactcttttg acagcaagtt tgtttaccag caaagaggtc
      661 ttgggccaat tgaagaagac accatcctgg tcattgaccc aaataatgct gaaatcctcc
      721 agtccagtgg caagaacctg ttttatttac cacacggctt gagcatagat acagatggaa
      781 attattgggt cacagatgtg gctctcc
//
