LOCUS       sequence-31177-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Shigetomi E, Kracun S, Sofroniew MV, Khakh BS.
  TITLE     A genetically targeted optical sensor to monitor calcium signals in 
            astrocyte processes.
  JOURNAL   Nat Neurosci. 2010 Jun;13(6):759-66.
  PUBMED    20495558
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci."; date: "2010-06"; volume: "13(6)"; pages: "759-66"
COMMENT     Sequence Label: pN1-Lck-GCaMP2
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..93)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(306..362)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      374..383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(390..404,405..413))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(417..449)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(453..470)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      596..605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(652..855)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(681..701)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttgctcacca tgctccctcc ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc
       61 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttc
      121 gaaagtttgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      181 atgggggtgt tctgctggta gtggtaggcg agctgcacgc cgccgtcctc gatgttgtgg
      241 cggatcttga agttcgcctt gatgccgttc ttctgcttgt cggccatgat atagacgttc
      301 tcgagtgagc tcagccgacc tatagctctg actgcgtgac ctgtcttatt ccacttacga
      361 cgtgatgagt cgaccatggt ggcgagatcc ttatcgtcat cgtcgtacag atcccgaccc
      421 atttgctgtc caccagtcat gctagccata ccatgatgat gatgatgatg agaaccccgc
      481 atggtggcga gatccggtgg atcaattcga agcttcggcg ggggataatg gcagttctca
      541 cacacgtcaa tgttctccat ccagtcatct tcagggtttg agctgcagcc acagcccatg
      601 gtggcagatc tgagtccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct
      661 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      721 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      781 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      841 ccaaaaccgc atcaccatgg taatagcgat gactaatacg ta
//
