LOCUS       sequence-311771-       11650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      425..434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1520..3013
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3110..3130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3195..3783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3248..3268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3666..3677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3786..3802)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3787..3803)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3893..4126
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4151..4169)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4152..4169)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4459..4478)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4692..4711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4811..4833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4871..4889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4957..5061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5062..5130,5131..5922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5280..5299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6093..6681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6582..6601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6835..6852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6922..6942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6927..7256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7107..7242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8390..8455
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8585..8605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9030..9164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9067..9086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9121..9140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9332..9965
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10012..10137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10634..10867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11052..11096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11381..11498
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggaa gagaggccac
      421 tagtgccacc atggcatcaa acagcctctt cagcacagtg acaccatgtc agcaaaactt
      481 cttttgggat ccgagcacca gccggcgctt cagccccccc tccagcagcc tgcagcccgg
      541 caaaatgagc gacgtgagcc cggtggtggc tgcgcaacag cagcagcaac agcagcagca
      601 gcaacagcag cagcagcagc agcaacagca gcagcagcag caggaggcgg cggcagccgc
      661 ggctgccgca gcggctgcag cggcggcggc tgcggcggcg gcggcggctg cggcggcggc
      721 agctgcagtg ccccggttgc ggccgcccca cgacaaccgc accatggtgg agacgtacgg
      781 ggtctggctc gtcgggtcca tggtttccaa aggagaggag gataatatgg ctataattaa
      841 agagtttatg cggttcaagg tgcatatgga gggatctgtg aacggtcacg agttcgaaat
      901 cgagggcgaa ggcgaggggc gaccctatga agggacacag acggccaaac tgaaggtgac
      961 caagggtggc cccctgcctt tcgcctggga catcctgtca ccccagttta tgtacggctc
     1021 taaggcttac gttaagcacc ctgccgatat tccggactac ttgaaactga gcttccctga
     1081 agggttcaag tgggaacggg taatgaattt tgaggatggc ggtgtagtca cagttaccca
     1141 ggacagctct cttcaggacg gagaatttat ctataaggtt aaacttcggg gcactaactt
     1201 cccatcagac ggccccgtga tgcagaagaa aactatgggc tgggaagcca gctcagaacg
     1261 catgtatccc gaggacggag cactgaaggg agaaatcaag cagcgactca agctgaagga
     1321 tgggggacat tatgatgctg aggtcaaaac cacctataag gccaagaaac ccgttcagct
     1381 tcctggggca tacaacgtga atatcaaact ggatattaca tcccataacg aagactatac
     1441 catcgtggaa cagtacgagc gggccgaggg cagacatagc acagggggca tggatgaatt
     1501 gtacaaggcg gccacgcgta tgaagatgga caaaaagact atagtttggt ttagaagaga
     1561 cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc
     1621 tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg
     1681 gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct
     1741 cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg
     1801 tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac
     1861 cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt
     1921 gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta
     1981 ctggaagaaa tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt
     2041 gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct
     2101 ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg
     2161 gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa
     2221 gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg
     2281 ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag
     2341 agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag
     2401 agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag
     2461 tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg
     2521 caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat
     2581 gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg
     2641 gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat
     2701 ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga
     2761 caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct
     2821 tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac
     2881 cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat
     2941 cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat
     3001 gatcggagca gcagcccggg atccaccggt cgccaccggt tcagggagcg gatccggctc
     3061 agggagtgcg gccggaactc ccacctgcaa catgcgtgac ggaggcggtc caaaaaagaa
     3121 gagaaaggta tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata
     3181 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3601 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3781 cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg
     3841 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3901 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3961 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4021 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4081 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     4141 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     4201 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     4261 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     4321 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     4381 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     4441 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     4501 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     4561 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     4621 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     4681 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     4741 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4801 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4861 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4921 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5221 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5281 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5701 gttgcaggaa cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5761 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6841 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6901 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7081 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7261 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     7321 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     7381 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     7441 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     7501 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     7561 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     7621 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     7681 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     7741 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     7801 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     7861 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     7921 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     7981 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     8041 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     8101 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     8161 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     8221 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     8281 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     8341 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     8401 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     8461 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     8521 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     8581 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     8641 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     8701 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     8761 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     8821 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     8881 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     8941 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     9001 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     9061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9121 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     9181 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     9241 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     9301 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     9361 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     9421 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     9481 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     9541 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     9601 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     9661 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     9721 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     9781 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9841 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9901 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9961 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    10021 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    10081 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    10141 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    10201 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    10261 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    10321 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    10381 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10441 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
    10501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    11041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    11101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    11161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    11221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    11281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    11341 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    11401 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    11461 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    11521 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
    11581 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
    11641 gctagctgca
//
