LOCUS       sequence-311775-       11699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1569..3062
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3159..3179
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3244..3832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3297..3317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3715..3726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3835..3851)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3836..3852)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3942..4175
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4200..4218)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4201..4218)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4508..4527)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4741..4760)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4860..4882
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4920..4938)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5006..5110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5111..5179,5180..5971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5329..5348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6142..6730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6631..6650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6884..6901
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6971..6991)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6976..7305
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7156..7291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8439..8504
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8634..8654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9079..9213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9116..9135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9170..9189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9381..10014
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10061..10186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10683..10916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11101..11145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11430..11547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatgtcccg agccggtagc tgggacatgg acggcctgcg agcggacgga ggcggagctg
      541 gaggagctcc cgcatcaagt agtagctcaa gtgtggccgc tgccgcggct agcggacaat
      601 gtagggggtt tttgtcagcg cctgtctttg cgggcacaca ttccgggagg gccgctgctg
      661 cggctgctgc tgctagtgga tttgcctatc ctgggacgag tgagcgcact ggttcatcat
      721 cctcatcttc atccagtgcg gtagtcgccg ctcggccaga agcaccacct gcaaaagagt
      781 gtccggcccc gacgccagct gcagcagcag cggcaccgcc ttcagctcct gcgggtaccg
      841 gaggcgggat ggtttccaaa ggagaggagg ataatatggc tataattaaa gagtttatgc
      901 ggttcaaggt gcatatggag ggatctgtga acggtcacga gttcgaaatc gagggcgaag
      961 gcgaggggcg accctatgaa gggacacaga cggccaaact gaaggtgacc aagggtggcc
     1021 ccctgccttt cgcctgggac atcctgtcac cccagtttat gtacggctct aaggcttacg
     1081 ttaagcaccc tgccgatatt ccggactact tgaaactgag cttccctgaa gggttcaagt
     1141 gggaacgggt aatgaatttt gaggatggcg gtgtagtcac agttacccag gacagctctc
     1201 ttcaggacgg agaatttatc tataaggtta aacttcgggg cactaacttc ccatcagacg
     1261 gccccgtgat gcagaagaaa actatgggct gggaagccag ctcagaacgc atgtatcccg
     1321 aggacggagc actgaaggga gaaatcaagc agcgactcaa gctgaaggat gggggacatt
     1381 atgatgctga ggtcaaaacc acctataagg ccaagaaacc cgttcagctt cctggggcat
     1441 acaacgtgaa tatcaaactg gatattacat cccataacga agactatacc atcgtggaac
     1501 agtacgagcg ggccgagggc agacatagca cagggggcat ggatgaattg tacaaggcgg
     1561 ccacgcgtat gaagatggac aaaaagacta tagtttggtt tagaagagac ctaaggattg
     1621 aggataatcc tgcattagca gcagctgctc acgaaggatc tgtttttcct gtcttcattt
     1681 ggtgtcctga agaagaagga cagttttatc ctggaagagc ttcaagatgg tggatgaaac
     1741 aatcacttgc tcacttatct caatccttga aggctcttgg atctgacctc actttaatca
     1801 aaacccacaa cacgatttca gcgatcttgg attgtatccg cgttaccggt gctacaaaag
     1861 tcgtctttaa ccacctctat gatcctgttt cgttagttcg ggaccatacc gtaaaggaga
     1921 agctggtgga acgtgggatc tctgtgcaaa gctacaatgg agatctattg tatgaaccgt
     1981 gggagatata ctgcgaaaag ggcaaacctt ttacgagttt caattcttac tggaagaaat
     2041 gcttagatat gtcgattgaa tccgttatgc ttcctcctcc ttggcggttg atgccaataa
     2101 ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga actagggctg gagaatgagg
     2161 ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc tccaggatgg agcaatgctg
     2221 ataagttact aaatgagttc atcgagaagc agttgataga ttatgcaaag aacagcaaga
     2281 aagttgttgg gaattctact tcactacttt ctccgtatct ccatttcggg gaaataagcg
     2341 tcagacacgt tttccagtgt gcccggatga aacaaattat atgggcaaga gataagaaca
     2401 gtgaaggaga agaaagtgca gatctttttc ttaggggaat cggtttaaga gagtattctc
     2461 ggtatatatg tttcaacttc ccgtttactc acgagcaatc gttgttgagt catcttcggt
     2521 ttttcccttg ggatgctgat gttgataagt tcaaggcctg gagacaaggc aggaccggtt
     2581 atccgttggt ggatgccgga atgagagagc tttgggctac cggatggatg cataacagaa
     2641 taagagtgat tgtttcaagc tttgctgtga agtttcttct ccttccatgg aaatggggaa
     2701 tgaagtattt ctgggataca cttttggatg ctgatttgga atgtgacatc cttggctggc
     2761 agtatatctc tgggagtatc cccgatggcc acgagcttga tcgcttggac aatcccgcgt
     2821 tacaaggcgc caaatatgac ccagaaggtg agtacataag gcaatggctt cccgagcttg
     2881 cgagattgcc aactgaatgg atccatcatc catgggacgc tcctttaacc gtactcaaag
     2941 cttctggtgt ggaactcgga acaaactatg cgaaacccat tgtagacatc gacacagctc
     3001 gtgagctact agctaaagct atttcaagaa cccgtgaagc acagatcatg atcggagcag
     3061 cagcccggga tccaccggtc gccaccggtt cagggagcgg atccggctca gggagtgcgg
     3121 ccggaactcc cacctgcaac atgcgtgacg gaggcggtcc aaaaaagaag agaaaggtat
     3181 gactgaggcc gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc
     3241 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3301 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3361 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3421 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3481 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3541 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3601 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3661 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3721 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3781 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3841 cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc
     3901 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3961 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     4021 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4081 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4141 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     4201 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     4261 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     4321 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     4381 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     4441 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     4501 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     4561 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     4621 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     4681 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     4741 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4801 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4861 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4921 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggaac
     5761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6841 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6901 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6961 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7021 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     7321 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     7381 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     7441 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     7501 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     7561 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     7621 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     7681 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     7741 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     7801 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     7861 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     7921 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     7981 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     8041 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     8101 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     8161 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     8221 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     8281 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     8341 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     8401 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     8461 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     8521 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     8581 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     8641 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     8701 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     8761 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     8821 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     8881 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     8941 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     9001 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     9061 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9181 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9241 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9301 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9361 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     9421 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     9481 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     9541 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     9601 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     9661 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     9721 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     9781 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     9841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
    10021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
    10081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    10141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    10201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    10261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    10321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
    10561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    10861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    11041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    11341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    11401 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
    11461 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    11521 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
    11581 tggatcgata agcttgatat cgaattcctg cagccccgat aaaataaaag attttattta
    11641 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgca
//
