LOCUS       sequence-311776-       11675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(-15A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(-15A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1545..3038
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3135..3155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3220..3808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3273..3293)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3691..3702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3811..3827)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3812..3828)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3918..4151
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4176..4194)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4177..4194)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4484..4503)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4717..4736)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4836..4858
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4896..4914)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4982..5086
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5087..5155,5156..5947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5305..5324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6118..6706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6607..6626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6860..6877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6947..6967)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6952..7281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7132..7267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8415..8480
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8610..8630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9055..9189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9092..9111)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9146..9165
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9357..9990
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10037..10162
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10659..10892
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11077..11121
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11406..11523
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatgtcccg agccggtagc tgggacatgg acggcctgcg agcggacgga ggcggagctg
      541 gaggagctcc cgcatcaagt agtagctcaa gtgtggccgc tgccgcggct agcggacaat
      601 gtagggggtt tttgtcagcg cctgtctttg cgggcacaca ttccgggagg agtggatttg
      661 cctatcctgg gacgagtgag cgcactggtt catcatcctc atcttcatcc agtgcggtag
      721 tcgccgctcg gccagaagca ccacctgcaa aagagtgtcc ggccccgacg ccagctgcag
      781 cagcagcggc accgccttca gctcctgcgg gtaccggagg cgggatggtt tccaaaggag
      841 aggaggataa tatggctata attaaagagt ttatgcggtt caaggtgcat atggagggat
      901 ctgtgaacgg tcacgagttc gaaatcgagg gcgaaggcga ggggcgaccc tatgaaggga
      961 cacagacggc caaactgaag gtgaccaagg gtggccccct gcctttcgcc tgggacatcc
     1021 tgtcacccca gtttatgtac ggctctaagg cttacgttaa gcaccctgcc gatattccgg
     1081 actacttgaa actgagcttc cctgaagggt tcaagtggga acgggtaatg aattttgagg
     1141 atggcggtgt agtcacagtt acccaggaca gctctcttca ggacggagaa tttatctata
     1201 aggttaaact tcggggcact aacttcccat cagacggccc cgtgatgcag aagaaaacta
     1261 tgggctggga agccagctca gaacgcatgt atcccgagga cggagcactg aagggagaaa
     1321 tcaagcagcg actcaagctg aaggatgggg gacattatga tgctgaggtc aaaaccacct
     1381 ataaggccaa gaaacccgtt cagcttcctg gggcatacaa cgtgaatatc aaactggata
     1441 ttacatccca taacgaagac tataccatcg tggaacagta cgagcgggcc gagggcagac
     1501 atagcacagg gggcatggat gaattgtaca aggcggccac gcgtatgaag atggacaaaa
     1561 agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca ttagcagcag
     1621 ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa gaaggacagt
     1681 tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac ttatctcaat
     1741 ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg atttcagcga
     1801 tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac ctctatgatc
     1861 ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt gggatctctg
     1921 tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc gaaaagggca
     1981 aaccttttac gagtttcaat tcttactgga agaaatgctt agatatgtcg attgaatccg
     2041 ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa gcgatttggg
     2101 cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc aatgcgttgt
     2161 taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat gagttcatcg
     2221 agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat tctacttcac
     2281 tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc cagtgtgccc
     2341 ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa agtgcagatc
     2401 tttttcttag gggaatcggt ttaagagagt attctcggta tatatgtttc aacttcccgt
     2461 ttactcacga gcaatcgttg ttgagtcatc ttcggttttt cccttgggat gctgatgttg
     2521 ataagttcaa ggcctggaga caaggcagga ccggttatcc gttggtggat gccggaatga
     2581 gagagctttg ggctaccgga tggatgcata acagaataag agtgattgtt tcaagctttg
     2641 ctgtgaagtt tcttctcctt ccatggaaat ggggaatgaa gtatttctgg gatacacttt
     2701 tggatgctga tttggaatgt gacatccttg gctggcagta tatctctggg agtatccccg
     2761 atggccacga gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa tatgacccag
     2821 aaggtgagta cataaggcaa tggcttcccg agcttgcgag attgccaact gaatggatcc
     2881 atcatccatg ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa
     2941 actatgcgaa acccattgta gacatcgaca cagctcgtga gctactagct aaagctattt
     3001 caagaacccg tgaagcacag atcatgatcg gagcagcagc ccgggatcca ccggtcgcca
     3061 ccggttcagg gagcggatcc ggctcaggga gtgcggccgg aactcccacc tgcaacatgc
     3121 gtgacggagg cggtccaaaa aagaagagaa aggtatgact gaggccgcga ctctagagtc
     3181 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     3241 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3301 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3361 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3421 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3481 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3541 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3601 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3661 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3721 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3781 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     3841 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     3901 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     3961 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4021 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4081 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4141 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     4201 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     4261 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     4321 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     4381 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     4441 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     4501 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     4561 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     4621 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     4681 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     4741 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4801 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4861 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4921 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4981 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5041 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5101 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5161 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5221 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5281 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5341 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5401 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5461 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5521 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5581 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5641 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5701 aatagactgg atggaggcgg ataaagttgc aggaacactt ctgcgctcgg cccttccggc
     5761 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5821 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5881 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5941 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6001 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6061 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6121 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6181 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6241 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6301 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6361 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6481 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6601 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6661 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6721 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6781 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6841 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6901 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     6961 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7021 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7081 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7141 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7201 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7261 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     7321 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     7381 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     7441 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     7501 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     7561 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     7621 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     7681 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     7741 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     7801 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     7861 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     7921 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     7981 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     8041 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     8101 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     8161 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     8221 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     8281 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     8341 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     8401 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     8461 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     8521 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     8581 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     8641 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     8701 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     8761 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     8821 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     8881 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     8941 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9001 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9061 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9121 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9181 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     9241 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     9301 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     9361 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     9421 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     9481 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     9541 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     9601 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     9661 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     9721 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     9781 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     9841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9961 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
    10021 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10081 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10141 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    10201 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    10261 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    10321 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    10381 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    10441 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    10501 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    10561 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    10621 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    10681 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    10741 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    10801 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    10861 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    10921 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    10981 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    11041 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    11101 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    11161 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    11221 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    11281 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    11341 gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc
    11401 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
    11461 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
    11521 tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa
    11581 ttcctgcagc cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
    11641 aagaccccac ctgtaggttt ggcaagctag ctgca
//
