LOCUS       sequence-311779-       11700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 
            NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..406
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1570..3063
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3160..3180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3245..3833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3298..3318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3716..3727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3836..3852)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3837..3853)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3943..4176
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4201..4219)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4202..4219)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4509..4528)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4742..4761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4861..4883
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4921..4939)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5007..5111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5112..5180,5181..5972)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5330..5349)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6143..6731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6632..6651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6885..6902
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6972..6992)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6977..7306
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7157..7292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8440..8505
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8635..8655
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9080..9214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9117..9136)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9171..9190
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9382..10015
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10062..10187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10684..10917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11102..11146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11431..11548
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tccgcgccag tcctccgaca gactgagtcg cccggggggg atctggagct
      421 ctcgagaatt ctcacgcgtc aagtggagca aggcaggtgg acagtggatc cggagctacc
      481 atgtcccgag ccggtagctg gatgggcctg cgagcgggag gcggagctgg aggagctccc
      541 gcatcaagta gtagctcaag tgtggccgct gccgcggcta gcggacaatg tagggggttt
      601 ttgtcagcgc ctgtctttgc gggcacacat tccgggaggg ccgctgctgc ggctgctgct
      661 gctgctgcag ccgcggcggc cgctagtgga tttgcctatc ctgggacgag tcgcactggt
      721 tcatcatcct catcttcatc cagtgcggta gtcgccgctc ggccagcacc acctgcaaaa
      781 tgtccggccc cgacgccagc tgcagcagca gcggcaccgc cttcagctcc tgcgggtacc
      841 ggaggcggga tggtttccaa aggagaggag gataatatgg ctataattaa agagtttatg
      901 cggttcaagg tgcatatgga gggatctgtg aacggtcacg agttcgaaat cgagggcgaa
      961 ggcgaggggc gaccctatga agggacacag acggccaaac tgaaggtgac caagggtggc
     1021 cccctgcctt tcgcctggga catcctgtca ccccagttta tgtacggctc taaggcttac
     1081 gttaagcacc ctgccgatat tccggactac ttgaaactga gcttccctga agggttcaag
     1141 tgggaacggg taatgaattt tgaggatggc ggtgtagtca cagttaccca ggacagctct
     1201 cttcaggacg gagaatttat ctataaggtt aaacttcggg gcactaactt cccatcagac
     1261 ggccccgtga tgcagaagaa aactatgggc tgggaagcca gctcagaacg catgtatccc
     1321 gaggacggag cactgaaggg agaaatcaag cagcgactca agctgaagga tgggggacat
     1381 tatgatgctg aggtcaaaac cacctataag gccaagaaac ccgttcagct tcctggggca
     1441 tacaacgtga atatcaaact ggatattaca tcccataacg aagactatac catcgtggaa
     1501 cagtacgagc gggccgaggg cagacatagc acagggggca tggatgaatt gtacaaggcg
     1561 gccacgcgta tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt
     1621 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt
     1681 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa
     1741 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc
     1801 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa
     1861 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag
     1921 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg
     1981 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa
     2041 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata
     2101 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag
     2161 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct
     2221 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag
     2281 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc
     2341 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac
     2401 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct
     2461 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg
     2521 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt
     2581 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga
     2641 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga
     2701 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg
     2761 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg
     2821 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt
     2881 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa
     2941 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct
     3001 cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca
     3061 gcagcccggg atccaccggt cgccaccggt tcagggagcg gatccggctc agggagtgcg
     3121 gccggaactc ccacctgcaa catgcgtgac ggaggcggtc caaaaaagaa gagaaaggta
     3181 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     3241 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3301 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3361 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3421 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3481 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3541 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3601 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3661 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3721 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3781 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3841 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     3901 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3961 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4021 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4081 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4141 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     4201 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     4261 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     4321 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     4381 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     4441 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     4501 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     4561 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     4621 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     4681 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     4741 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4801 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4861 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4921 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4981 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5041 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5101 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5161 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5221 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5281 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5341 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5401 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5461 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5521 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5581 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5641 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5701 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggaa
     5761 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5821 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5881 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5941 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6001 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6781 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6841 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6901 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6961 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7081 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7141 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7201 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7261 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     7321 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     7381 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     7441 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     7501 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     7561 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     7621 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     7681 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     7741 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     7801 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     7861 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     7921 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     7981 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     8041 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     8101 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     8161 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     8221 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     8281 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     8341 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     8401 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     8461 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     8521 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     8581 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     8641 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     8701 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     8761 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     8821 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     8881 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     8941 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     9001 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     9061 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     9121 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9181 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     9241 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     9301 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9361 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     9421 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     9481 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     9541 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     9601 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     9661 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     9721 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     9781 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     9841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    10021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    10081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    10141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    10201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    10261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    10321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
    10561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    11041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    11101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    11161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    11221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    11281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    11341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    11401 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    11461 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    11521 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
    11581 ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt
    11641 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca
//
