LOCUS       sequence-311782-        9800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 
            NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 9800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-MCS-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..9800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(498..500,501..503,504..1214)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(543..564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(804..823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1151..1172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1224..1244
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1345..1933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1398..1418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1816..1827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1936..1952)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1937..1953)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2043..2276
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2301..2319)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2302..2319)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2609..2628)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2842..2861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2961..2983
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3021..3039)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3107..3211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3212..3280,3281..4072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3430..3449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4243..4831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4732..4751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4985..5002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5072..5092)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5077..5406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5257..5392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6540..6605
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6735..6755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7180..7314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7217..7236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7271..7290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7482..8115
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8162..8287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8784..9017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9202..9246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9531..9648
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggaa gagaggccac
      421 tagtcgcgac cacgcgccgg cgggacgaga cgcgatcgcg gacggcaacg tacgggttct
      481 ggctcgtcgg ttcccatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      601 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      661 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct
      721 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      781 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      901 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      961 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1081 tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga
     1141 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1201 acgagctgta caagggaggc ggtccaaaaa agaagagaaa ggtatgagcg gccgcaactc
     1261 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcaggcatgc
     1321 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     1381 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     1441 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     1501 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     1561 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     1621 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     1681 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     1741 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     1801 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     1861 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     1921 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     1981 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     2041 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     2101 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     2161 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     2221 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     2281 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     2341 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     2401 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     2461 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     2521 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     2581 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     2641 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     2701 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     2761 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     2821 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     2881 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2941 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3001 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3061 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3841 ggcggataaa gttgcaggaa cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4381 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4921 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4981 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5041 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     5101 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5161 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5221 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5341 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5401 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     5461 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     5521 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     5581 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     5641 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     5701 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     5761 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     5821 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     5881 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     5941 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     6001 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     6061 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     6121 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     6181 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     6241 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     6301 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     6361 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     6421 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     6481 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     6541 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     6601 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     6661 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     6721 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     6781 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     6841 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     6901 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     6961 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     7021 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     7081 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     7141 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     7201 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7261 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     7321 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     7381 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     7441 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     7501 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7561 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     7621 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     7681 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     7741 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     7801 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     7861 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     7921 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8101 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8161 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8221 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8281 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8341 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8401 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8461 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8521 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8581 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     8641 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8701 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8761 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8821 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8881 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8941 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     9001 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     9061 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     9121 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     9181 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9241 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9301 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9361 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     9421 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     9481 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
     9541 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9601 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9661 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga
     9721 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     9781 ggtttggcaa gctagctgca
//
