LOCUS       sequence-311784-       11616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 
            NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-MED1trunc-IDR-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..11616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(2314..2316,2317..2319,2320..3030)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2359..2380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2620..2639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2967..2988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3040..3060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3161..3749
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3214..3234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3632..3643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3752..3768)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3753..3769)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3859..4092
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4117..4135)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4118..4135)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4425..4444)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4658..4677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4777..4799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4837..4855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4923..5027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5028..5096,5097..5888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5246..5265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6059..6647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6548..6567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6801..6818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6888..6908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6893..7222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7073..7208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8356..8421
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8551..8571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8996..9130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9033..9052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9087..9106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9298..9931
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9978..10103
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10600..10833
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11018..11062
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11347..11464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 catcacagtg gtagtcaggg tcctttactg accactgggg acttagggaa agaaaagact
      481 caaaagaggg taaaggaagg caatggcacc agtaatagta ctctctcggg gcccggatta
      541 gacagcaaac cagggaagcg cagtcggacc ccttctaatg atgggaaaag caaagataag
      601 cctccaaagc ggaagaaggc agacactgag ggaaagtctc catctcatag ttcttctaac
      661 agacctttta ccccacctac cagtacaggt ggatctaaat cgccaggcag tgcaggaaga
      721 tctcagactc ccccaggtgt tgccacacca cccattccca aaatcactat tcagattcct
      781 aagggaacag tgatggtggg caagccttcc tctcacagtc agtataccag cagtggttct
      841 gtgtcttcct caggcagcaa aagccaccat agccattctt cctcctcttc ctcatctgct
      901 tccacctcag ggaagatgaa aagcagtaaa tcagaaggtt catcaagttc caagttaagt
      961 agcagtatgt attctagcca ggggtcttct ggatctagcc agtccaaaaa ttcatcccag
     1021 tctgggggga agccaggctc ctctcccata accaagcatg gactgagcag tggctctagc
     1081 agcaccaaga tgaaacctca aggaaagcca tcatcactta tgaatccttc tttaagtaaa
     1141 ccaaacatat ccccttctca ttcaaggcca cctggaggct ctgacaagct tgcctctcca
     1201 atgaagcctg ttcctggaac tcctccatcc tctaaagcca agtcccctat cagttcaggt
     1261 tctggtggtt ctcatatgtc tggaactagt tcaagctctg gcatgaagtc atcttcaggg
     1321 ttaggatcct caggctcgtt gtcccagaaa actcccccat catctaattc ctgtacggca
     1381 tcttcctcct ccttttcctc aagtggctct tccatgtcat cctctcagaa ccagcatggg
     1441 agttctaaag gaaaatctcc cagcagaaac aagaagccgt ccttgacagc tgtcatagat
     1501 aaactgaagc atggggttgt caccagtggc cctgggggtg aagacccact ggacggccag
     1561 atgggggtga gcacaaattc ttccagccat cctatgtcct ccaaacataa catgtcagga
     1621 ggagagtttc agggcaagcg tgagaaaagt gataaagaca aatcaaaggt ttccacctcc
     1681 gggagttcag tggattcttc taagaagacc tcagagtcaa aaaatgtggg gagcacaggt
     1741 gtggcaaaaa ttatcatcag taagcatgat ggaggctccc ctagcattaa agccaaagtg
     1801 actttgcaga aacctgggga aagtagtgga gaagggctta ggcctcaaat ggcttcttct
     1861 aaaaactatg gctctccact catcagtggt tccactccaa agcatgagcg tggctctccc
     1921 agccatagta agtcaccagc atataccccc cagaatctgg acagtgaaag tgagtcaggc
     1981 tcctccatag cagagaaatc ttatcagaat agtcccagct cagacgatgg tatccgacca
     2041 cttccagaat acagcacaga gaaacataag aagcacaaaa aggaaaagaa gaaagtaaaa
     2101 gacaaagata gggaccgaga ccgggacaaa gaccgagaca agaaaaaatc tcatagcatc
     2161 aagccagaga gttggtccaa atcacccatc tcttcagacc agtccttgtc tatgacaagt
     2221 aacacaatct tatctgcaga cagaccctca aggctcagcc cagactttat gattggggag
     2281 gaagatgatg atcttggagg tagcggagga attatggtga gcaagggcga ggagctgttc
     2341 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2401 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2461 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     2521 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2581 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2641 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2701 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2761 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2821 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2881 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     2941 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3001 atcactctcg gcatggacga gctgtacaag ggaggcggtc caaaaaagaa gagaaaggta
     3061 tgagcggccg caactcccac ctgcaacatg cgtgactgac tgaggccgcg actctagagt
     3121 cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa
     3181 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3241 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3301 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3361 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3421 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3481 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3541 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3601 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3661 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3721 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt
     3781 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3841 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3901 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3961 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4021 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4081 aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat
     4141 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
     4201 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
     4261 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
     4321 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
     4381 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     4441 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     4501 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     4561 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     4621 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     4681 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4741 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4801 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4861 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4921 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4981 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5041 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5101 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5161 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5221 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5281 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5341 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5401 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5461 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5521 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5581 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5641 taatagactg gatggaggcg gataaagttg caggaacact tctgcgctcg gcccttccgg
     5701 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5761 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5821 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5881 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6841 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     6901 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6961 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7021 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7201 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     7261 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     7321 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     7381 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     7441 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     7501 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     7561 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     7621 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     7681 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     7741 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     7801 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     7861 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     7921 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     7981 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     8041 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     8101 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     8161 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     8221 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     8281 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     8341 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     8401 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     8461 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     8521 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     8581 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     8641 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     8701 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     8761 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     8821 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     8881 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     8941 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     9001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9121 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     9181 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     9241 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     9301 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     9361 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     9421 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     9481 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9541 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     9601 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     9661 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     9721 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     9781 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9841 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9901 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9961 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
    10021 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
    10081 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
    10141 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
    10201 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
    10261 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
    10321 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
    10381 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
    10441 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    10501 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    10561 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    10621 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    10681 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    10741 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    10801 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    10861 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    10921 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    10981 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    11041 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    11101 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    11161 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    11221 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    11281 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
    11341 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    11401 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    11461 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
    11521 attcctgcag ccccgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
    11581 aaagacccca cctgtaggtt tggcaagcta gctgca
//
