LOCUS       sequence-311786-       11693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-MCS-MED1long-IDR-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..11693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(2391..2393,2394..2396,2397..3107)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2436..2457)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2697..2716)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3044..3065
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3117..3137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3238..3826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3291..3311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3709..3720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3829..3845)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3830..3846)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3936..4169
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4194..4212)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4195..4212)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4502..4521)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4735..4754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4854..4876
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4914..4932)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5000..5104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5105..5173,5174..5965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5323..5342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6136..6724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6625..6644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6878..6895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6965..6985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6970..7299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7150..7285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8433..8498
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8628..8648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9073..9207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9110..9129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9164..9183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9375..10008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10055..10180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10677..10910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11095..11139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11424..11541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggaa gagaggccac
      421 tagtcgcgac cacgcgccgg cgggacgaga cgcgatcgcg agctaccatg gagcatcaca
      481 gtggtagtca gggtccttta ctgaccactg gggacttagg gaaagaaaag actcaaaaga
      541 gggtaaagga aggcaatggc accagtaata gtactctctc ggggcccgga ttagacagca
      601 aaccagggaa gcgcagtcgg accccttcta atgatgggaa aagcaaagat aagcctccaa
      661 agcggaagaa ggcagacact gagggaaagt ctccatctca tagttcttct aacagacctt
      721 ttaccccacc taccagtaca ggtggatcta aatcgccagg cagtgcagga agatctcaga
      781 ctcccccagg tgttgccaca ccacccattc ccaaaatcac tattcagatt cctaagggaa
      841 cagtgatggt gggcaagcct tcctctcaca gtcagtatac cagcagtggt tctgtgtctt
      901 cctcaggcag caaaagccac catagccatt cttcctcctc ttcctcatct gcttccacct
      961 cagggaagat gaaaagcagt aaatcagaag gttcatcaag ttccaagtta agtagcagta
     1021 tgtattctag ccaggggtct tctggatcta gccagtccaa aaattcatcc cagtctgggg
     1081 ggaagccagg ctcctctccc ataaccaagc atggactgag cagtggctct agcagcacca
     1141 agatgaaacc tcaaggaaag ccatcatcac ttatgaatcc ttctttaagt aaaccaaaca
     1201 tatccccttc tcattcaagg ccacctggag gctctgacaa gcttgcctct ccaatgaagc
     1261 ctgttcctgg aactcctcca tcctctaaag ccaagtcccc tatcagttca ggttctggtg
     1321 gttctcatat gtctggaact agttcaagct ctggcatgaa gtcatcttca gggttaggat
     1381 cctcaggctc gttgtcccag aaaactcccc catcatctaa ttcctgtacg gcatcttcct
     1441 cctccttttc ctcaagtggc tcttccatgt catcctctca gaaccagcat gggagttcta
     1501 aaggaaaatc tcccagcaga aacaagaagc cgtccttgac agctgtcata gataaactga
     1561 agcatggggt tgtcaccagt ggccctgggg gtgaagaccc actggacggc cagatggggg
     1621 tgagcacaaa ttcttccagc catcctatgt cctccaaaca taacatgtca ggaggagagt
     1681 ttcagggcaa gcgtgagaaa agtgataaag acaaatcaaa ggtttccacc tccgggagtt
     1741 cagtggattc ttctaagaag acctcagagt caaaaaatgt ggggagcaca ggtgtggcaa
     1801 aaattatcat cagtaagcat gatggaggct cccctagcat taaagccaaa gtgactttgc
     1861 agaaacctgg ggaaagtagt ggagaagggc ttaggcctca aatggcttct tctaaaaact
     1921 atggctctcc actcatcagt ggttccactc caaagcatga gcgtggctct cccagccata
     1981 gtaagtcacc agcatatacc ccccagaatc tggacagtga aagtgagtca ggctcctcca
     2041 tagcagagaa atcttatcag aatagtccca gctcagacga tggtatccga ccacttccag
     2101 aatacagcac agagaaacat aagaagcaca aaaaggaaaa gaagaaagta aaagacaaag
     2161 atagggaccg agaccgggac aaagaccgag acaagaaaaa atctcatagc atcaagccag
     2221 agagttggtc caaatcaccc atctcttcag accagtcctt gtctatgaca agtaacacaa
     2281 tcttatctgc agacagaccc tcaaggctca gcccagactt tatgattggg gaggaagatg
     2341 atgatcttgg aggtagcgga gcgtacgggt tctggctcgt cggttcccat atggtgagca
     2401 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2461 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2521 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2581 ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2641 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2701 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2761 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2821 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2881 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2941 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     3001 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3061 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga ggcggtccaa
     3121 aaaagaagag aaaggtatga gcggccgcaa ctcccacctg caacatgcgt gactgactga
     3181 ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat
     3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3601 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     3841 cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     3901 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3961 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4021 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4081 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4141 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     4201 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     4261 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     4321 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     4381 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     4441 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     4501 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     4561 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     4621 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     4681 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     4741 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4801 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4861 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4921 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaacacttct
     5761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6961 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7021 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7201 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     7321 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     7381 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     7441 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     7501 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     7561 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     7621 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     7681 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     7741 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     7801 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     7861 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     7921 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     7981 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     8041 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     8101 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     8161 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     8221 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     8281 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     8341 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     8401 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     8461 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     8521 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     8581 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     8641 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     8701 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     8761 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     8821 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     8881 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     8941 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     9001 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     9061 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9121 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9181 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     9241 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     9301 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     9361 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     9421 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     9481 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     9541 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     9601 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     9661 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     9721 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     9781 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     9841 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9901 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9961 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10021 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    10081 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10141 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10201 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10261 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10321 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10381 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10441 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10501 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
    10561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    10621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    10681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    10741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    10801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    10861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    10921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    10981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    11041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    11101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    11161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    11221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    11281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    11341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
    11401 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    11461 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    11521 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc
    11581 gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta tttagtctcc
    11641 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct gca
//
