LOCUS       sequence-311787-        5376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low phosphate induction of pntAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Li S, Ye Z, Moreb EA, Hennigan JN, Castellanos DB, Yang T, Lynch MD
  TITLE     Dynamic control over feedback regulatory mechanisms improves NADPH 
            flux and xylitol biosynthesis in engineered E. coli.
  JOURNAL   Metab Eng. 2021 Jan 15. pii: S1096-7176(21)00013-6. doi: 
            10.1016/j.ymben.2021.01.005.
  PUBMED    33460820
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2021 Jan 15. pii: S1096-7176(21)00013-6. doi: 
            10.1016/j.ymben.2021.01.005."
COMMENT     Sequence Label: pCDF-pntAB
FEATURES             Location/Qualifiers
     source          1..5376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..80
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      96..123
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      3597..4335
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        4383..4474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4475..5266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatac acagctaaca ccacgtcgtc cctatctgct gccctaggtc tatgagtggt
      181 tgctggataa ctctttctga caccttacta tcttacaaat gtaacaaaaa agttattttt
      241 ctgtaattcg agcatgtcat gttaccccgc gagcataaaa cgcgtatatt cagggagacc
      301 acaacggttt ccctctacaa ataattttgt ttaactttgg aaaaaggaga tataccatgc
      361 gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca acgccaaaaa
      421 cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc gcgggtcaac
      481 tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta gaagggaata
      541 gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat gaaattgcgt
      601 tactgaatcc tgggacaacg ctggtgagtt ttatctggcc tgcgcagaat ccggaattaa
      661 tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg ccgcgtatct
      721 cacgcgcaca atcgctggac gcactaagct cgatggcgaa catcgccggt tatcgcgcca
      781 ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaattact gcggccggga
      841 aagtgccacc ggcaaaagtg atggtgattg gtgcgggtgt tgcaggtctg gccgccattg
      901 gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg gaagtgaaag
      961 aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag gaagctggca
     1021 gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa atggaactct
     1081 ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt ccaggcaaac
     1141 cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc agtgtgattg
     1201 tcgacctggc agcccaaaac ggcggcaact gtgaatacac cgtgccgggt gaaatcttca
     1261 ctacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt ctgccgacgc
     1321 aatcctcaca gctttacggc acaaacctcg ttaatctgct gaaactgttg tgcaaagaga
     1381 aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg accgtgatcc
     1441 gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag ccgcaggcgg
     1501 cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tacctgctca ccgtggcgta
     1561 aatacgcgtt gatggcgctg gcaatcattc tttttggctg gatggcaagc gttgcgccga
     1621 aagaattcct tgggcacttc accgttttcg cgctggcctg cgttgtcggt tattacgtgg
     1681 tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggtcaccaac gcgatttcag
     1741 ggattattgt tgtcggagca ctgttgcaga ttggccaggg cggctgggtt agcttcctta
     1801 gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc gtgactcagc
     1861 gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg attagttaca
     1921 gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct ttcgaaacat
     1981 gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc gttaatcgca
     2041 accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat ggtcattggt
     2101 ggggcaattg gtatccgtct ggcgaagaaa gttgaaatga ccgaaatgcc agaactggtg
     2161 gcgatcctgc atagcttcgt gggtctggcg gcagtgctgg ttggctttaa cagctatctg
     2221 catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga agtgttcctc
     2281 ggtatcttca tcggggcggt aacgttcacg ggttcggtgg tggcgttcgg caaactgtgt
     2341 ggcaagattt cgtctaaacc attgatgctg ccaaaccgtc acaaaatgaa cctggcggct
     2401 ctggtcgttt ccttcctgct gctgattgta tttgttcgca cggacagcgt cggcctgcaa
     2461 gtgctggcat tgctgataat gaccgcaatt gcgctggtat tcggctggca tttagtcgcc
     2521 tccatcggtg gtgcagatat gccagtggtg gtgtcgatgc tgaactcgta ctccggctgg
     2581 gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac cggtgcgctg
     2641 gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa ccgttccttt
     2701 atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga tgatcaggaa
     2761 gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa aaactcccat
     2821 tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata tcctgtcgct
     2881 gaaattactg agaaattgcg cgctcgtggt attaatgtgc gtttcggtat ccacccggtc
     2941 gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt accgtatgac
     3001 atcgtgctgg aaatggacga gatcaatgat gactttgctg ataccgatac cgtactggtg
     3061 attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag tccgattgct
     3121 ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa acgttcgatg
     3181 aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac ccacatgctg
     3241 tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaaga cgaattctct
     3301 agatatcgct caatactgac catttaaatc atacctgacc taatgcagct ggcacgacag
     3361 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca
     3421 ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3481 gtgggcgcgg ggcatgacta acatgagaat tacaacttat atcgtatggg gctgacttca
     3541 ggtgctacat ttgaagagat aaattgcact gaaatctaga gcggttcagt agaaaagatc
     3601 aaaggatctt cttgagatcc tttttttctg cgcgtaatct tttgccctgt aaacgaaaaa
     3661 accacctggg gaggtggttt gatcgaacgt taagtcagtt ggggaactgc ttaaccgtgg
     3721 taactggctt tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg
     3781 cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta
     3841 ccaatggctg ctgccagtgg cgttttaccg tgcttttccg ggttggactc aagtgaacag
     3901 ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg
     3961 gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac
     4021 ttcccgtaag ggagaaaggc ggaacaggta tccggtaaac ggcagggtcg gaacaggaga
     4081 gcgcaagagg gagcgacccg ccggaaacgg tggggatctt taagtcctgt cgggtttcgc
     4141 ccgtactgtc agattcatgg ttgagcctca cggctcccac agatgcaccg gaaaagcgtc
     4201 tgtttatgtg aactctggca ggagggcgga gcctatggaa aaacgccacc ggcgcggccc
     4261 tgctgttttg cctcacatgt tagtcccctg cttatccacg gaatctgtgg gtaactttgt
     4321 atgtgtccgc agcgcccgcc gcagtctcac gcccggagcg tagcgaccga gtgagctagc
     4381 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4441 ataaatgctt caataatatt gaaaaaggaa gagtatgagg gaagcggtga tcgccgaagt
     4501 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4561 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4621 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4681 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4741 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4801 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     4861 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     4921 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     4981 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     5041 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     5101 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5161 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc acagatcagt tggaagaatt
     5221 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
     5281 ttcaagccga ggggccgcaa gatccggcca cgatgaccag tccagttacg ctggagtctg
     5341 aggctcgtcc tgaatgatat caagcttgaa ttcgtt
//
