LOCUS       sequence-311788-       10238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13-IDR-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..10238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(936..938,939..941,942..1652)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(981..1002)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1242..1261)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1589..1610
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1662..1682
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1783..2371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1836..1856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2254..2265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2374..2390)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2375..2391)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2481..2714
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2739..2757)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2740..2757)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3047..3066)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3280..3299)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3399..3421
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3459..3477)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3545..3649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3650..3718,3719..4510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3868..3887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4681..5269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5170..5189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5423..5440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5510..5530)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5515..5844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5695..5830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6978..7043
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7173..7193
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7618..7752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7655..7674)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7709..7728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7920..8553
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8600..8725
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9222..9455
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9640..9684
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9969..10086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatgtcccg agccggtagc tgggacatgg acggcctgcg agcggacgga ggcggagctg
      541 gaggagctcc cgcatcaagt agtagctcaa gtgtggccgc tgccgcggct agcggacaat
      601 gtagggggtt tttgtcagcg cctgtctttg cgggcacaca ttccgggagg gccgctgctg
      661 cggctgctgc tgctgctgca gccgcggcgg ccgctagtgg atttgcctat cctgggacga
      721 gtgagcgcac tggttcatca tcctcatctt catccagtgc ggtagtcgcc gctcggccag
      781 aagcaccacc tgcaaaagag tgtccggccc cgacgccagc tgcagcagca gcggcaccgc
      841 cttcagctcc tgcgggtacc ggaggcggga tggtttccaa aggagaggag gataatatgg
      901 ctataattaa agagtttatg cggttcaagg tgcatatggt gagcaagggc gaggagctgt
      961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
     1141 tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1381 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1441 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1501 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
     1561 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1621 ggatcactct cggcatggac gagctgtaca agggaggcgg tccaaaaaag aagagaaagg
     1681 tatgagcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga
     1741 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     1801 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1861 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1921 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     1981 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2041 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2101 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2161 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2221 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2281 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2341 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
     2401 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2461 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     2521 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2581 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2641 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2701 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     2761 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     2821 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     2881 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     2941 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     3001 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     3061 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     3121 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     3181 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     3241 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     3301 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3361 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3421 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3481 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4201 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4261 attaatagac tggatggagg cggataaagt tgcaggaaca cttctgcgct cggcccttcc
     4321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5341 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5401 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5461 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     5521 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5581 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5641 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5701 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5761 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5821 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     5881 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     5941 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     6001 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     6061 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     6121 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     6181 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     6241 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     6301 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     6361 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     6421 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     6481 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     6541 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     6601 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     6661 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     6721 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     6781 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     6841 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     6901 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     6961 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     7021 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     7081 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     7141 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     7201 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     7261 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     7321 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     7381 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     7441 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     7501 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7561 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7621 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7681 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7741 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     7801 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     7861 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     7921 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     7981 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     8041 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     8101 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8161 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     8221 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     8281 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     8341 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     8401 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8461 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8521 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8581 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8641 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8701 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8761 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8821 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8881 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8941 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     9001 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     9061 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9121 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9181 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9241 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9301 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9361 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9421 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9481 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9541 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9601 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9661 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9721 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9781 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9841 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     9901 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     9961 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10021 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10081 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
    10141 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
    10201 tgaaagaccc cacctgtagg tttggcaagc tagctgca
//
