LOCUS       sequence-31179-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T
  TITLE     Activation of p53-dependent growth suppression in human cells by 
            mutations in PTEN or PIK3CA.
  JOURNAL   Mol Cell Biol. 2007 Jan . 27(2):662-77.
  PUBMED    17060456
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Jan . 27(2):662-77."
COMMENT     Sequence Label: pLP-LNCX-PIK3CA-H1047R
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..158
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             187..840
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
     primer_bind     complement(253..272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtcaggtggc acttttcggg gaatgtgcgc ggaaccccta tttgtttatt tttctaaata
       61 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattgg
      121 atctataact tcgtataatg tatgctatac gaagttatgg atctttcagg agctaaggaa
      181 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      241 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      301 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      361 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa
      421 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      481 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      541 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      601 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
      661 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
      721 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
      781 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
      841 ggcgtaattt gatatcgagc tcgcttggac tctgtg
//
