LOCUS       sequence-311790-        9715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor cytoplasmic cell labeling with a genome-integrating Tol2 
            transposon vector (randomly expressing 3 tdTomato, mCerulean or 
            mEYFP upon Cre recombination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng 
            S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin 
            X, Livet J
  TITLE     Multiplex cell and lineage tracking with combinatorial labels.
  JOURNAL   Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.
  PUBMED    24507188
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.12"; journalName: "Neuron"; date: "2014-02-5-
            5"; volume: "81"; issue: "3"; pages: "505-20"
COMMENT     Sequence Label: Tol2-CAG::Cytbow
FEATURES             Location/Qualifiers
     source          1..9715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..22)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    68..123
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(122..141)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(505..667,668..679)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(758..775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(929..1517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1009..1028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1688..2479,2480..2548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2311..2330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2549..2653)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(2687..2698,2699..2886)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        2890..3269
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3547..4563
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4487..4509
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4571..4590
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4626..4659)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(4666..4699)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4706..4739)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5141..5150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5880..6087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6087..6120)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      6131..6140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6137..7567
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    7694..7775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7731..7750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7785..7804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(7818..7851)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      7858..7867
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7864..7866,7867..7869,7870..8583)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    8593..8800
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8800..8833)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      8847..8856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8853..8855,8856..8858,8859..9572)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    9590..9623
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
       61 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
      121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
      181 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
      241 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
      301 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
      361 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
      421 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tgcggccgcc
      481 ttaaacaaga atctctagtt ttctttcttg cttttacttt tacttcctta atactcaagt
      541 acaattttaa tggagtactt ttttactttt actcaagtaa gattctagcc agatactttt
      601 acttttaatt gagtaaaatt ttccctaagt acttgtactt tcacttgagt aaaatttttg
      661 agtacttttt acacctctga tcgatggatc cgctgcatta atgaatcggc caacgcgcgg
      721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2641 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgaccaga ggtgtaaagt
     2701 acttgagtaa ttttacttga ttactgtact taagtattat ttttggggat ttttacttta
     2761 cttgagtaca attaaaaatc aatactttta cttttactta attacatttt tttagaaaaa
     2821 aaagtacttt ttactcctta caattttatt tacagtcaaa aagtacttat tttttggaga
     2881 tcacttgtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     2941 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3001 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3061 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3121 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3181 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3241 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     3301 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     3361 gtgcagcgat gggggcgggg gggggggggg ggcgcgccrg gsgggggggg gggggggggg
     3421 ggggggggsg gggsgrggsg grgaggtgcg gcggcagcca atcagagcgg cgcgctccga
     3481 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     3541 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     3601 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     3661 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     3721 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     3781 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     3841 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     3901 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     3961 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     4021 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     4081 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     4141 ggggcggggc cgcctcgggc cggggrgggc tcgggggagg ggcgcggcgg cccccggagc
     4201 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     4261 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     4321 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     4381 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     4441 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     4501 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     4561 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctag
     4621 ctagcataac ttcgtatagt ataccttata cgaagttatc tcgagataac ttcgtatagg
     4681 atactttata cgaagttata ctgtcataac ttcgtatagc atacattata cgaagttatc
     4741 ccgggccacc atgcctgagc cagccaagag cgcaccagca ccaaagaaag ggtccaagaa
     4801 agccgtcaca aaagcacaga aaaaggatgg gaaaaagagg aagagaagcc ggaaagagtc
     4861 ttacagtatc tacgtgtata aggtcctgaa acaggtgcat cccgacactg gtatcagctc
     4921 caaggcaatg ggcattatga actctttcgt gaatgacatc tttgagagga ttgccggaga
     4981 agctagtaga ctcgcacact acaacaagag gagcaccatc accagcaggg agattcagac
     5041 tgccgtgcgg ctgctcctgc caggagagct ggcaaagcat gccgtgtcag aagggactaa
     5101 ggccgtcacc aaatatacca gcgccaagga cccaccggtc gccaccatgg tctccaaggg
     5161 agaggaactg ttcacagggg tggtccccat cctcgtggaa ctggacggag atgtcaatgg
     5221 gcacaagttt agtgtgaggg gcgagggaga aggggatgct acaaacggca agctgaccct
     5281 gaagttcatc tgcaccacag gcaagctgcc agtgccctgg cctacactcg tcactaccct
     5341 gtcacatggc gtgcagtgtt ttgccagata ccccgaccac atgaagcagc atgatttctt
     5401 taaaagcgcc atgccagagg gttacgtgca ggaacggacc atcttcttta aggacgatgg
     5461 gacttataaa acccgcgctg aggtgaagtt cgaaggagac acactcgtca accgaatcga
     5521 gctgaagggg gtggacttca aagaagatgg taatattctc ggccacaagc tggagtacaa
     5581 ctttaatagc cataacatct atatcatggc tgtcaagcag aaaaacggaa tcaaggtgaa
     5641 cttcaagatt cggcacaatg tggaagacgg ctccgtccag ctggcagatc attaccagca
     5701 gaacactccc atcggtgacg gcccagtcct cctgcccgat tctcactatc tctcaaccca
     5761 gagcgtgctg tccaaggacc ctaacgagaa acgcgatcat atggtgctgc tggagtttcg
     5821 gaccgccgcc gggatcacac tgggtatgga cgagctgtac aagtaatgag atatctcgac
     5881 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5941 ggaaggtgcc actctcactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6001 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6061 ggaagacaat agcaggcatg ctggggataa cttcgtatag tataccttat acgaagttat
     6121 tggatcatcc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
     6181 caaggtgcgg atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     6241 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     6301 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     6361 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     6421 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctcca
     6481 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     6541 cgtaatgcag aagaagacga tgggctggga ggcctccacc gagcgcctgt acccccgcga
     6601 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     6661 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     6721 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     6781 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag
     6841 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat
     6901 gcgcttcaag gtgcggatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     6961 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     7021 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     7081 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     7141 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     7201 cctccaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     7261 cggccccgta atgcagaaga agacgatggg ctgggaggcc tccaccgagc gcctgtaccc
     7321 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     7381 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     7441 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     7501 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
     7561 caagtaaagt gtgccgccgc gaccctagat cataatcagc cataccacat ttgtagaggt
     7621 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     7681 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7741 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7801 catcaatgta attttaaata acttcgtata ggatacttta tacgaagtta ttccggagcc
     7861 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7921 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     7981 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     8041 accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     8101 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     8161 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     8221 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     8281 cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaag
     8341 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     8401 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     8461 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     8521 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     8581 taactagttc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     8641 cttccttgac cctggaaggt gccactctca ctgtcctttc ctaataaaat gaggaaattg
     8701 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     8761 agggggagga ttgggaagac aatagcaggc atgctgggga taacttcgta tagcatacat
     8821 tatacgaagt tatagatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     8881 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     8941 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     9001 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     9061 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     9121 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     9181 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     9241 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     9301 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     9361 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     9421 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca
     9481 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     9541 tcactctcgg catggacgag ctgtacaagt aaagcggccg catttaaatg aagttcctat
     9601 tctctagaaa gtataggaac ttcctcgagc ctaggaattc actcctcagg tgcaggctgc
     9661 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actga
//
