LOCUS       sequence-311797-       10252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor nuclear-restricted labeling with a genome-integrating 
            Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, 
            mCherry or mCerulean upon Cre recombination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng 
            S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin 
            X, Livet J
  TITLE     Multiplex cell and lineage tracking with combinatorial labels.
  JOURNAL   Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.
  PUBMED    24507188
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.12"; journalName: "Neuron"; date: "2014-02-5-
            5"; volume: "81"; issue: "3"; pages: "505-20"
COMMENT     Sequence Label: Tol2-CAG::Nucbow
FEATURES             Location/Qualifiers
     source          1..10252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..22)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    68..123
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(122..141)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(505..667,668..679)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(758..775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(929..1517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1009..1028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1688..2479,2480..2548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2311..2330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2549..2653)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(2687..2698,2699..2886)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        2890..3269
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3547..4563
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4487..4509
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4571..4590
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4626..4659)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(4666..4699)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4706..4739)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5141..5150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5880..6087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6087..6120)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             join(6532..6534,6535..6537,6538..7251)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(7581..7614)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      8013..8022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8019..8729
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8164..8182)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8432..8451
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8746..8953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8953..8986)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      9388..9397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9394..9396,9397..9399,9400..10113)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    10127..10160
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
       61 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
      121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
      181 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
      241 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
      301 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
      361 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
      421 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tgcggccgcc
      481 ttaaacaaga atctctagtt ttctttcttg cttttacttt tacttcctta atactcaagt
      541 acaattttaa tggagtactt ttttactttt actcaagtaa gattctagcc agatactttt
      601 acttttaatt gagtaaaatt ttccctaagt acttgtactt tcacttgagt aaaatttttg
      661 agtacttttt acacctctga tcgatggatc cgctgcatta atgaatcggc caacgcgcgg
      721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2641 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgaccaga ggtgtaaagt
     2701 acttgagtaa ttttacttga ttactgtact taagtattat ttttggggat ttttacttta
     2761 cttgagtaca attaaaaatc aatactttta cttttactta attacatttt tttagaaaaa
     2821 aaagtacttt ttactcctta caattttatt tacagtcaaa aagtacttat tttttggaga
     2881 tcacttgtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     2941 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3001 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3061 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3121 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3181 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3241 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     3301 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     3361 gtgcagcgat gggggcgggg gggggggggg ggsgcgccrg gggggggggg gggggggggg
     3421 gggggggggg gggsgrggcg grgaggtgcg gcggcagcca atcagagcgg cgcgctccga
     3481 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     3541 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     3601 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     3661 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     3721 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     3781 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     3841 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     3901 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     3961 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     4021 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     4081 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     4141 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     4201 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     4261 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     4321 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     4381 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     4441 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     4501 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     4561 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctag
     4621 ctagcataac ttcgtatagt ataccttata cgaagttatc tcgagataac ttcgtatagg
     4681 atactttata cgaagttata ctgtcataac ttcgtatagc atacattata cgaagttatc
     4741 ccgggccacc atgcctgagc cagccaagag cgcaccagca ccaaagaaag ggtccaagaa
     4801 agccgtcaca aaagcacaga aaaaggatgg gaaaaagagg aagagaagcc ggaaagagtc
     4861 ttacagtatc tacgtgtata aggtcctgaa acaggtgcat cccgacactg gtatcagctc
     4921 caaggcaatg ggcattatga actctttcgt gaatgacatc tttgagagga ttgccggaga
     4981 agctagtaga ctcgcacact acaacaagag gagcaccatc accagcaggg agattcagac
     5041 tgccgtgcgg ctgctcctgc caggagagct ggcaaagcat gccgtgtcag aagggactaa
     5101 ggccgtcacc aaatatacca gcgccaagga cccaccggtc gccaccatgg tctccaaggg
     5161 agaggaactg ttcacagggg tggtccccat cctcgtggaa ctggacggag atgtcaatgg
     5221 gcacaagttt agtgtgaggg gcgagggaga aggggatgct acaaacggca agctgaccct
     5281 gaagttcatc tgcaccacag gcaagctgcc agtgccctgg cctacactcg tcactaccct
     5341 gtcacatggc gtgcagtgtt ttgccagata ccccgaccac atgaagcagc atgatttctt
     5401 taaaagcgcc atgccagagg gttacgtgca ggaacggacc atcttcttta aggacgatgg
     5461 gacttataaa acccgcgctg aggtgaagtt cgaaggagac acactcgtca accgaatcga
     5521 gctgaagggg gtggacttca aagaagatgg taatattctc ggccacaagc tggagtacaa
     5581 ctttaatagc cataacatct atatcatggc tgtcaagcag aaaaacggaa tcaaggtgaa
     5641 cttcaagatt cggcacaatg tggaagacgg ctccgtccag ctggcagatc attaccagca
     5701 gaacactccc atcggtgacg gcccagtcct cctgcccgat tctcactatc tctcaaccca
     5761 gagcgtgctg tccaaggacc ctaacgagaa acgcgatcat atggtgctgc tggagtttcg
     5821 gaccgccgcc gggatcacac tgggtatgga cgagctgtac aagtaatgag atatctcgac
     5881 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5941 ggaaggtgcc actctcactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6001 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6061 ggaagacaat agcaggcatg ctggggataa cttcgtatag tataccttat acgaagttat
     6121 tggatctccg ccaccatgcc agaaccagca aaaagtgctc cagcaccaaa gaaagggtct
     6181 aagaaagccg ttaccaaagc ccagaagaag gacggcaaaa agcggaaacg gagtcgcaaa
     6241 gagagctaca gcatctacgt ttataaagtt ctgaaacagg tccatccaga tacaggaatt
     6301 agttcaaaag ccatgggcat catgaactcc tttgtgaacg acatttttga acgcattgcc
     6361 ggtgaagcct cccgactggc acactataac aaacgatcta caattacttc acgggagatt
     6421 caaaccgccg tcagactcct gctgcccggc gaactcgcta aacacgctgt aagcgaagga
     6481 acaaaggccg tgaccaaata caccagcgct aaagatcccc ctgtagccac gatggtgagc
     6541 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     6601 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     6661 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     6721 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     6781 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     6841 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     6901 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     6961 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     7021 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     7081 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     7141 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     7201 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtataagta agcggccgca
     7261 ccccctttcc tgctcttgcc tgtgaacaat ggttaattgt tcccaagaga gcatctgtca
     7321 gttgttggca aaatgataga catttgaaaa tctgtcttct gacaaataaa aagcatctat
     7381 gttcactgca atgatgtttt aaattatttg tctgtgtcat agaagggttt atgctaagtt
     7441 ttcaagatac aaagaagtga gggttcaggt ctgaccttgg ggaaataaat gaattacact
     7501 tcaaattgtg ttgtcagcta agcagcagta gccacagatc ctattgccat gccctaaaca
     7561 ctcagagaaa acttaattaa ataacttcgt ataggatact ttatacgaag ttatccgcca
     7621 ccatgcctga gcccgccaag tctgctccag cccctaaaaa gggttctaag aaggctgtga
     7681 ctaaggcgca gaagaaggat ggtaagaagc gtaagcgcag ccgcaaggag agctattcta
     7741 tctatgtgta taaggttctg aagcaggtcc accccgacac cggcatctca tccaaggcca
     7801 tggggatcat gaactccttc gtcaacgaca tcttcgagcg catcgcgggc gaggcttctc
     7861 gcctggctca ctacaataag cgcagcacca tcacctccag ggagattcag acggctgtgc
     7921 gcctgctgct gcctggggag ctggctaagc atgctgtgtc cgagggcact aaggcagtta
     7981 ccaagtacac tagcgccaag gatccaccag tcgccaccat ggtgagcaag ggcgaggagg
     8041 ataacatggc catcatcaag gagttcatgc gcttcaaggt ccacatggag ggctccgtga
     8101 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     8161 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     8221 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     8281 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     8341 gcgtggtgac cgtgacccag gactcctccc tccaggacgg cgagttcatc tacaaggtga
     8401 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag acgatgggct
     8461 gggaggcctc ctccgagcgc atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     8521 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     8581 ccaagaagcc cgtgcagctc cccggcgcct acaacgtcaa catcaagttg gacatcacct
     8641 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     8701 ccggcggcat ggacgagctg tacaagtaag tcgacagtac ttcgactgtg ccttctagtt
     8761 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     8821 tcactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     8881 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     8941 ggcatgctgg ggataacttc gtatagcata cattatacga agttatccgc caccatgcct
     9001 gagccagcca agagcgcacc agcaccaaag aaagggtcca agaaagccgt cacaaaagca
     9061 cagaaaaagg atgggaaaaa gaggaagaga agccggaaag agtcttacag tatctacgtg
     9121 tataaggtcc tgaaacaggt gcatcccgac actggtatca gctccaaggc aatgggcatt
     9181 atgaactctt tcgtgaatga catctttgag aggattgccg gagaagctag tagactcgca
     9241 cactacaaca agaggagcac catcaccagc agggagattc agactgccgt gcggctgctc
     9301 ctgccaggag agctggcaaa gcatgccgtg tcagaaggga ctaaggccgt caccaaatat
     9361 accagcgcca aggacccacc ctccggagcc accatggtga gcaagggcga ggagctgttc
     9421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     9481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     9541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
     9601 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     9661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     9721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     9781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac
     9841 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     9901 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     9961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
    10021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
    10081 atcactctcg gcatggacga gctgtacaag taactagtat ttaaatgaag ttcctattct
    10141 ctagaaagta taggaacttc ctcgagccta ggaattcact cctcaggtgc aggctgccta
    10201 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact ga
//
