LOCUS       sequence-311799-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor membrane-restricted labeling with a genome-integrating 
            Tol2 transposon vector (randomly expressing palmitoylated mCherry, 
            mEYFP or mCerulean upon Cre recombination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng 
            S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin 
            X, Livet J
  TITLE     Multiplex cell and lineage tracking with combinatorial labels.
  JOURNAL   Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.
  PUBMED    24507188
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.12"; journalName: "Neuron"; date: "2014-02-5-
            5"; volume: "81"; issue: "3"; pages: "505-20"
COMMENT     Sequence Label: Tol2-CAG::Palmbow
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(32..194,195..206)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(285..302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(456..1044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(536..555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1215..2006,2007..2075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1838..1857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2076..2180)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(2214..2225,2226..2413)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        2417..2796
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2798..3073
                     /label=chicken beta-actin promoter
     intron          3074..4090
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4014..4036
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4098..4117
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4153..4186)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(4193..4226)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4233..4266)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4668..4677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5407..5614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5614..5647)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             5769..6479
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5914..5932)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6182..6201
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6606..6727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6731..6764)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             join(6880..6882,6883..6885,6886..7599)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    7722..7803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7841..7874)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7990..7992,7993..7995,7996..8709)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    8832..8913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8956..8989
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggccgcctta aacaagaatc tctagttttc tttcttgctt ttacttttac ttccttaata
       61 ctcaagtaca attttaatgg agtacttttt tacttttact caagtaagat tctagccaga
      121 tacttttact tttaattgag taaaattttc cctaagtact tgtactttca cttgagtaaa
      181 atttttgagt actttttaca cctctgatcg atggatccgc tgcattaatg aatcggccaa
      241 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      301 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      361 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gaccagaggt
     2221 gtaaagtact tgagtaattt tacttgatta ctgtacttaa gtattatttt tggggatttt
     2281 tactttactt gagtacaatt aaaaatcaat acttttactt ttacttaatt acattttttt
     2341 agaaaaaaaa gtacttttta ctccttacaa ttttatttac agtcaaaaag tacttatttt
     2401 ttggagatca cttgtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
     2461 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2521 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2581 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2641 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2701 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2761 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     2821 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     2881 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgccrgggg gggggggggg
     2941 gggggggggg sggggsgggg sgaggcggag aggtgcggcg gcagccaatc agagcggcgc
     3001 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     3061 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
     3121 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     3181 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     3241 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     3301 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     3361 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     3421 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     3481 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     3541 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     3601 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     3661 gccgggcggg gcggggccgc ctcgggccgg ggrgggctcg ggggaggggc gcggcggccc
     3721 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     3781 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     3841 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     3901 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     3961 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     4021 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     4081 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     4141 attctagcta gcataacttc gtatagtata ccttatacga agttatctcg agataacttc
     4201 gtataggata ctttatacga agttatactg tcataacttc gtatagcata cattatacga
     4261 agttatcccg ggccaccatg cctgagccag ccaagagcgc accagcacca aagaaagggt
     4321 ccaagaaagc cgtcacaaaa gcacagaaaa aggatgggaa aaagaggaag agaagccgga
     4381 aagagtctta cagtatctac gtgtataagg tcctgaaaca ggtgcatccc gacactggta
     4441 tcagctccaa ggcaatgggc attatgaact ctttcgtgaa tgacatcttt gagaggattg
     4501 ccggagaagc tagtagactc gcacactaca acaagaggag caccatcacc agcagggaga
     4561 ttcagactgc cgtgcggctg ctcctgccag gagagctggc aaagcatgcc gtgtcagaag
     4621 ggactaaggc cgtcaccaaa tataccagcg ccaaggaccc accggtcgcc accatggtct
     4681 ccaagggaga ggaactgttc acaggggtgg tccccatcct cgtggaactg gacggagatg
     4741 tcaatgggca caagtttagt gtgaggggcg agggagaagg ggatgctaca aacggcaagc
     4801 tgaccctgaa gttcatctgc accacaggca agctgccagt gccctggcct acactcgtca
     4861 ctaccctgtc acatggcgtg cagtgttttg ccagataccc cgaccacatg aagcagcatg
     4921 atttctttaa aagcgccatg ccagagggtt acgtgcagga acggaccatc ttctttaagg
     4981 acgatgggac ttataaaacc cgcgctgagg tgaagttcga aggagacaca ctcgtcaacc
     5041 gaatcgagct gaagggggtg gacttcaaag aagatggtaa tattctcggc cacaagctgg
     5101 agtacaactt taatagccat aacatctata tcatggctgt caagcagaaa aacggaatca
     5161 aggtgaactt caagattcgg cacaatgtgg aagacggctc cgtccagctg gcagatcatt
     5221 accagcagaa cactcccatc ggtgacggcc cagtcctcct gcccgattct cactatctct
     5281 caacccagag cgtgctgtcc aaggacccta acgagaaacg cgatcatatg gtgctgctgg
     5341 agtttcggac cgccgccggg atcacactgg gtatggacga gctgtacaag taatgagata
     5401 tctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5461 tgaccctgga aggtgccact ctcactgtcc tttcctaata aaatgaggaa attgcatcgc
     5521 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5581 aggattggga agacaatagc aggcatgctg gggataactt cgtatagtat accttatacg
     5641 aagttattgg atcatccgcc accatgctgt gctgcatccg ccgcaccaag cccgtggaga
     5701 agaacgaaga ggccgaccag gagctgcaaa gcaccgtgcc ccgcgcccgc gaccctcccg
     5761 tggccacgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     5821 gcttcaaggt ccacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     5881 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     5941 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     6001 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     6061 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     6121 tccaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     6181 gccccgtaat gcagaagaag acgatgggct gggaggcctc ctccgagcgc atgtaccccg
     6241 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     6301 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctc cccggcgcct
     6361 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     6421 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     6481 gtgtgccgcc gcgaccctag atcataatca gccataccac atttgtagag gttttacttg
     6541 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     6601 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6661 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6721 taatttaaat ataacttcgt ataggatact ttatacgaag ttattgccgc caccatgctg
     6781 tgctgcatcc gccgcaccaa gcccgtggag aagaacgaag aggccgacca ggagctgcaa
     6841 agcaccgtgc cccgcgcccg cgaccctccc gtggccacga tggtgagcaa gggcgaggag
     6901 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     6961 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     7021 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     7081 ggcctccagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7141 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7201 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7261 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7321 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     7381 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     7441 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag
     7501 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     7561 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gtggccgcga ctccagatca
     7621 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     7681 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     7741 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7801 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ataacttcgt atagcataca
     7861 ttatacgaag ttatagccgc caccatgctg tgctgcatcc gccgcaccaa gcccgtggag
     7921 aagaacgaag aggccgacca ggagctgcaa agcaccgtgc cccgcgcccg cgaccctccc
     7981 gtggccacga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     8041 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     8101 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     8161 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     8221 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     8281 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     8341 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     8401 ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag
     8461 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     8521 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     8581 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     8641 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     8701 tacaagtaaa gcgggcgcga ctccagatca taatcagcca taccacattt gtagaggttt
     8761 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8821 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8941 tcaatgtatc cgctcgaagt tcctattctc tagaaagtat aggaacttcc ccagcttggc
     9001 gcgcctagtt attaatgc
//
