LOCUS       sequence-311800-        9426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor mitochondrial-restricted labeling with a 
            genome-integrating Tol2 transposon vector (randomly expressing 
            mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng 
            S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin 
            X, Livet J
  TITLE     Multiplex cell and lineage tracking with combinatorial labels.
  JOURNAL   Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.
  PUBMED    24507188
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.12"; journalName: "Neuron"; date: "2014-02-5-
            5"; volume: "81"; issue: "3"; pages: "505-20"
COMMENT     Sequence Label: Tol2-CAG::Mitbow
FEATURES             Location/Qualifiers
     source          1..9426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(39..201,202..213)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(292..309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(463..1051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(543..562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1222..2013,2014..2082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1845..1864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2083..2187)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(2221..2232,2233..2420)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        2424..2803
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3081..4097
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4021..4043
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4105..4124
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4160..4193)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(4200..4233)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4240..4273)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4675..4684
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5414..5525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5622..5655)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             5672..5746
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(5789..5791,5792..6505)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     protein_bind    complement(6827..6860)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             6878..6952
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7001..7708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7143..7161)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7411..7430
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7719..7830
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(7927..7960)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7976..8050
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      8096..8105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8102..8104,8105..8107,8108..8821)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    8835..8868
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8963..8982)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9028..9083
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9082..9101)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 agcttgcggc cgccttaaac aagaatctct agttttcttt cttgctttta cttttacttc
       61 cttaatactc aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct
      121 agccagatac ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt
      181 gagtaaaatt tttgagtact ttttacacct ctgatcgatg gatccgctgc attaatgaat
      241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac
     2221 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
     2281 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
     2341 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
     2401 ttattttttg gagatcactt gtcgacattg attattgact agttattaat agtaatcaat
     2461 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2521 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2581 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2641 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2701 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2761 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     2821 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     2881 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg ccrggggggg
     2941 gggggggggg gggggggggg ggsggggsgr ggcggagagg tgcggcggca gccaatcaga
     3001 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     3061 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     3121 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     3181 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     3241 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     3301 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     3361 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     3421 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     3481 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     3541 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     3601 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     3661 tgggggtgcc gggcggggcg gggccgcctc gggccggggr gggctcgggg gaggggcgcg
     3721 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     3781 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     3841 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     3901 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     3961 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     4021 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     4081 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     4141 gcaaagaatt ctagctagca taacttcgta tagtatacct tatacgaagt tatctcgaga
     4201 taacttcgta taggatactt tatacgaagt tatactgtca taacttcgta tagcatacat
     4261 tatacgaagt tatcccgggc caccatgcct gagccagcca agagcgcacc agcaccaaag
     4321 aaagggtcca agaaagccgt cacaaaagca cagaaaaagg atgggaaaaa gaggaagaga
     4381 agccggaaag agtcttacag tatctacgtg tataaggtcc tgaaacaggt gcatcccgac
     4441 actggtatca gctccaaggc aatgggcatt atgaactctt tcgtgaatga catctttgag
     4501 aggattgccg gagaagctag tagactcgca cactacaaca agaggagcac catcaccagc
     4561 agggagattc agactgccgt gcggctgctc ctgccaggag agctggcaaa gcatgccgtg
     4621 tcagaaggga ctaaggccgt caccaaatat accagcgcca aggacccacc ggtcgccacc
     4681 atggtctcca agggagagga actgttcaca ggggtggtcc ccatcctcgt ggaactggac
     4741 ggagatgtca atgggcacaa gtttagtgtg aggggcgagg gagaagggga tgctacaaac
     4801 ggcaagctga ccctgaagtt catctgcacc acaggcaagc tgccagtgcc ctggcctaca
     4861 ctcgtcacta ccctgtcaca tggcgtgcag tgttttgcca gataccccga ccacatgaag
     4921 cagcatgatt tctttaaaag cgccatgcca gagggttacg tgcaggaacg gaccatcttc
     4981 tttaaggacg atgggactta taaaacccgc gctgaggtga agttcgaagg agacacactc
     5041 gtcaaccgaa tcgagctgaa gggggtggac ttcaaagaag atggtaatat tctcggccac
     5101 aagctggagt acaactttaa tagccataac atctatatca tggctgtcaa gcagaaaaac
     5161 ggaatcaagg tgaacttcaa gattcggcac aatgtggaag acggctccgt ccagctggca
     5221 gatcattacc agcagaacac tcccatcggt gacggcccag tcctcctgcc cgattctcac
     5281 tatctctcaa cccagagcgt gctgtccaag gaccctaacg agaaacgcga tcatatggtg
     5341 ctgctggagt ttcggaccgc cgccgggatc acactgggta tggacgagct gtacaagtaa
     5401 tgagatatct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5461 ccttccttga ccctggaagg tgccactctc actgtccttt cctaataaaa tgaggaaatt
     5521 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5581 aagggggagg attgggaaga caatagcagg catgctgggg tataacttcg tatagtatac
     5641 cttatacgaa gttattgagc tcgccgccac catgtccgtc ctgacgccgc tgctgctgcg
     5701 gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttgcg
     5761 atcgggcgat ccacccgtcg ccaccgctgt gagcaagggc gaggagctgt tcaccggggt
     5821 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5881 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5941 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     6001 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6061 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     6121 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     6181 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     6241 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     6301 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6361 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     6421 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6481 cggcatggac gagctgtaca agtaagttta aacaccccct ttcctgctct tgcctgtgaa
     6541 caatggttaa ttgttcccaa gagagcatct gtcagttgtt ggcaaaatga tagacatttg
     6601 aaaatctgtc ttctgacaaa taaaaagcat ctatgttcac tgcaatgatg ttttaaatta
     6661 tttgtctgtg tcatagaagg gtttatgcta agttttcaag atacaaagaa gtgagggttc
     6721 aggtctgacc ttggggaaat aaatgaatta cacttcaaat tgtgttgtca gctaagcagc
     6781 agtagccaca gatcctattg ccatgcccta aacactcaga gaaaacataa cttcgtatag
     6841 gatactttat acgaagttat ttaattaagc cgccaccatg tccgtcctga cgccgctgct
     6901 gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca agatccattc
     6961 gttgagtact ggcgatccac ccgtcgccac cggatccgct gtgagcaagg gcgaggagga
     7021 taacatggcc atcatcaagg agttcatgcg cttcaaggtc cacatggagg gctccgtgaa
     7081 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     7141 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     7201 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     7261 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     7321 cgtggtgacc gtgacccagg actcctccct ccaggacggc gagttcatct acaaggtgaa
     7381 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga cgatgggctg
     7441 ggaggcctcc tccgagcgca tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     7501 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     7561 caagaagccc gtgcagctcc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     7621 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     7681 cggcggcatg gacgagctgt acaagtaagt cgactcgact gtgccttcta gttgccagcc
     7741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctctcactgt
     7801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     7861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     7921 tggggtataa cttcgtatag catacattat acgaagttat cacgtggccg ccaccatgtc
     7981 cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc
     8041 gcgcgccaag atccattcgt tgggtaccgg cgatccaccc gtcgccacct ccggagccac
     8101 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     8161 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     8221 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     8281 cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     8341 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     8401 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     8461 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     8521 caagctggag tacaacgcca tcagcgacaa cgtctatatc accgccgaca agcagaagaa
     8581 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     8641 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     8701 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     8761 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     8821 actagtattt aaatgaagtt cctattctct agaaagtata ggaacttcct cgagcctagg
     8881 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     8941 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     9001 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     9061 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     9121 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     9181 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     9241 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     9301 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     9361 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     9421 agccca
//
