LOCUS       sequence-311801-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor cytoplasmic cell labeling with a genome-integrating 
            piggyBac transposon vector (randomly expressing tdTomato, 
            mTurquoise2 or mEYFP upon Cre recombination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng 
            S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin 
            X, Livet J
  TITLE     Multiplex cell and lineage tracking with combinatorial labels.
  JOURNAL   Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.
  PUBMED    24507188
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.12"; journalName: "Neuron"; date: "2014-02-5-
            5"; volume: "81"; issue: "3"; pages: "505-20"
COMMENT     Sequence Label: PB-CAG::Cytbow
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..647
                     /label=chicken beta-actin promoter
     intron          648..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1727..1760)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(1767..1800)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1807..1840)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2242..2251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2981..3188
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3188..3221)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      3232..3241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3238..4668
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    4795..4876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4919..4952)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      4960..4969
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4966..4968,4969..4971,4972..5685)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    5708..5915
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5915..5948)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5962..5971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5968..5970,5971..5973,5974..6687)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    6705..6738
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6833..6852)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6898..6953
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6952..6971)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   7335..7397
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        7440..7544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7545..7613,7614..8405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7763..7782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8576..9164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9065..9084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9318..9335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    9403..9537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(9543..9738)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9557..9692
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    9797..9818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9833..9838,9839..9856,9857..9863)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9871..9887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9876..9898
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9895..9911
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9895..9911
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   9937..9971
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tgcaggtagt tcataatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      421 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      481 gggggggggg gsgcgcgcca ggsggggcgg ggsggggcgr ggggcggggc ggggcgaggc
      541 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
      601 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcta gctagcataa cttcgtatag
     1741 tataccttat acgaagttat ctcgagataa cttcgtatag gatactttat acgaagttat
     1801 actgtcataa cttcgtatag catacattat acgaagttat cccgggccac catgcctgag
     1861 ccagccaaga gcgcaccagc accaaagaaa gggtccaaga aagccgtcac aaaagcacag
     1921 aaaaaggatg ggaaaaagag gaagagaagc cggaaagagt cttacagtat ctacgtgtat
     1981 aaggtcctga aacaggtgca tcccgacact ggtatcagct ccaaggcaat gggcattatg
     2041 aactctttcg tgaatgacat ctttgagagg attgccggag aagctagtag actcgcacac
     2101 tacaacaaga ggagcaccat caccagcagg gagattcaga ctgccgtgcg gctgctcctg
     2161 ccaggagagc tggcaaagca tgccgtgtca gaagggacta aggccgtcac caaatatacc
     2221 agcgccaagg acccaccggt cgccaccatg gtctccaagg gagaggaact gttcacaggg
     2281 gtggtcccca tcctcgtgga actggacgga gatgtcaatg ggcacaagtt tagtgtgagg
     2341 ggcgagggag aaggggatgc tacaaacggc aagctgaccc tgaagttcat ctgcaccaca
     2401 ggcaagctgc cagtgccctg gcctacactc gtcactaccc tgtcacatgg cgtgcagtgt
     2461 tttgccagat accccgacca catgaagcag catgatttct ttaaaagcgc catgccagag
     2521 ggttacgtgc aggaacggac catcttcttt aaggacgatg ggacttataa aacccgcgct
     2581 gaggtgaagt tcgaaggaga cacactcgtc aaccgaatcg agctgaaggg ggtggacttc
     2641 aaagaagatg gtaatattct cggccacaag ctggagtaca actttaatag ccataacatc
     2701 tatatcatgg ctgtcaagca gaaaaacgga atcaaggtga acttcaagat tcggcacaat
     2761 gtggaagacg gctccgtcca gctggcagat cattaccagc agaacactcc catcggtgac
     2821 ggcccagtcc tcctgcccga ttctcactat ctctcaaccc agagcgtgct gtccaaggac
     2881 cctaacgaga aacgcgatca tatggtgctg ctggagtttc ggaccgccgc cgggatcaca
     2941 ctgggtatgg acgagctgta caagtaatga gatatctcga ctgtgccttc tagttgccag
     3001 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactctcact
     3061 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3121 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3181 gctggggata acttcgtata gtatacctta tacgaagtta ttggatcatc cgccaccatg
     3241 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg gatggagggc
     3301 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     3361 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     3421 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     3481 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     3541 gacggcggtc tggtgaccgt gacccaggac tcctccctcc aggacggcac gctgatctac
     3601 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacg
     3661 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     3721 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     3781 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     3841 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     3901 caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc
     3961 tcctccgagg acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcggatg
     4021 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     4081 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     4141 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     4201 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     4261 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctccagga cggcacgctg
     4321 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     4381 aagacgatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     4441 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     4501 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     4561 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     4621 ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagtaaag tgtgccgccg
     4681 cgaccctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4741 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4801 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4861 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt aattttaaat
     4921 aacttcgtat aggatacttt atacgaagtt attccggtcg ccaccatggt gagcaagggc
     4981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5041 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5161 tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     5221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5401 tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     5461 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     5521 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5581 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5641 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     5701 agttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5761 ttgaccctgg aaggtgccac tctcactgtc ctttcctaat aaaatgagga aattgcatcg
     5821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5881 gaggattggg aagacaatag caggcatgct ggggataact tcgtatagca tacattatac
     5941 gaagttatag atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     6001 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     6061 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     6121 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     6181 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6241 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6301 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     6361 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6421 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6481 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6541 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac
     6601 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6661 ctcggcatgg acgagctgta caagtaaagc ggccgcattt aaatgaagtt cctattctct
     6721 agaaagtata ggaacttcct cgagcctagg aattcactcc tcaggtgcag gctgcctatc
     6781 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     6841 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     6901 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     6961 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     7021 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     7081 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     7141 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     7201 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     7261 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgcgcct agttattaat
     7321 gcggccgctc gcgacatgcg tcaattttac gcatgattat ctttaacgta cgtcacaata
     7381 tgattatctt tctagggtta agcccggtcg acccaggtgg cacttttcgg ggaaatgtgc
     7441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7561 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7621 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7681 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7741 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7801 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7861 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7921 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7981 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8041 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8101 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8161 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8221 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8281 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8341 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8401 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8701 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag cggatccaga catgataaga
     9421 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     9481 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttagc
     9541 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     9601 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     9661 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     9721 gctgactaat tgagatgcgg atccgctggc acgacaggtt tcccgactgg aaagcgggca
     9781 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9841 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9901 acagctatga ccatgattac gccaagctcg atttaaccct agaaagatag tctgcgtaaa
     9961 attgacgcat gcatcc
//
