LOCUS       sequence-311805-       10499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor nuclear-restricted labeling with a genome-integrating 
            piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, 
            mCherry or mCerulean upon Cre recombination).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng 
            S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin 
            X, Livet J
  TITLE     Multiplex cell and lineage tracking with combinatorial labels.
  JOURNAL   Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.
  PUBMED    24507188
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.12"; journalName: "Neuron"; date: "2014-02-5-
            5"; volume: "81"; issue: "3"; pages: "505-20"
COMMENT     Sequence Label: PB-CAG::Nucbow
FEATURES             Location/Qualifiers
     source          1..10499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1136..1343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(1343..1376)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             join(1788..1790,1791..1793,1794..2507)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(2837..2870)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      3269..3278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3275..3985
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3420..3438)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3688..3707
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4002..4209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4209..4242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4644..4653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4650..4652,4653..4655,4656..5369)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    5383..5416
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(5511..5530)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5576..5631
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5630..5649)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   6013..6075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        6118..6222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6223..6291,6292..7083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6441..6460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7254..7842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7743..7762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7996..8013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8081..8215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8105..8124)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8159..8178
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8221..8416)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8235..8370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    8475..8496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8511..8516,8517..8534,8535..8541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8549..8565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8554..8576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8573..8589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8573..8589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8615..8649
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        8721..9024
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9026..9301
                     /label=chicken beta-actin promoter
     intron          9302..10318
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10242..10264
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10326..10345
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(10381..10414)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(10421..10454)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(10461..10494)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccaccatgc ctgagccagc caagagcgca ccagcaccaa agaaagggtc caagaaagcc
       61 gtcacaaaag cacagaaaaa ggatgggaaa aagaggaaga gaagccggaa agagtcttac
      121 agtatctacg tgtataaggt cctgaaacag gtgcatcccg acactggtat cagctccaag
      181 gcaatgggca ttatgaactc tttcgtgaat gacatctttg agaggattgc cggagaagct
      241 agtagactcg cacactacaa caagaggagc accatcacca gcagggagat tcagactgcc
      301 gtgcggctgc tcctgccagg agagctggca aagcatgccg tgtcagaagg gactaaggcc
      361 gtcaccaaat ataccagcgc caaggaccca ccggtcgcca ccatggtctc caagggagag
      421 gaactgttca caggggtggt ccccatcctc gtggaactgg acggagatgt caatgggcac
      481 aagtttagtg tgaggggcga gggagaaggg gatgctacaa acggcaagct gaccctgaag
      541 ttcatctgca ccacaggcaa gctgccagtg ccctggccta cactcgtcac taccctgtca
      601 catggcgtgc agtgttttgc cagatacccc gaccacatga agcagcatga tttctttaaa
      661 agcgccatgc cagagggtta cgtgcaggaa cggaccatct tctttaagga cgatgggact
      721 tataaaaccc gcgctgaggt gaagttcgaa ggagacacac tcgtcaaccg aatcgagctg
      781 aagggggtgg acttcaaaga agatggtaat attctcggcc acaagctgga gtacaacttt
      841 aatagccata acatctatat catggctgtc aagcagaaaa acggaatcaa ggtgaacttc
      901 aagattcggc acaatgtgga agacggctcc gtccagctgg cagatcatta ccagcagaac
      961 actcccatcg gtgacggccc agtcctcctg cccgattctc actatctctc aacccagagc
     1021 gtgctgtcca aggaccctaa cgagaaacgc gatcatatgg tgctgctgga gtttcggacc
     1081 gccgccggga tcacactggg tatggacgag ctgtacaagt aatgagatat ctcgactgtg
     1141 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     1201 ggtgccactc tcactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     1261 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     1321 gacaatagca ggcatgctgg ggataacttc gtatagtata ccttatacga agttattgga
     1381 tctccgccac catgccagaa ccagcaaaaa gtgctccagc accaaagaaa gggtctaaga
     1441 aagccgttac caaagcccag aagaaggacg gcaaaaagcg gaaacggagt cgcaaagaga
     1501 gctacagcat ctacgtttat aaagttctga aacaggtcca tccagataca ggaattagtt
     1561 caaaagccat gggcatcatg aactcctttg tgaacgacat ttttgaacgc attgccggtg
     1621 aagcctcccg actggcacac tataacaaac gatctacaat tacttcacgg gagattcaaa
     1681 ccgccgtcag actcctgctg cccggcgaac tcgctaaaca cgctgtaagc gaaggaacaa
     1741 aggccgtgac caaatacacc agcgctaaag atccccctgt agccacgatg gtgagcaagg
     1801 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1861 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1921 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     1981 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     2041 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2101 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2161 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2221 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2281 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2341 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     2401 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2461 tgaccgccgc cgggatcact ctcggcatgg acgagctgta taagtaagcg gccgcacccc
     2521 ctttcctgct cttgcctgtg aacaatggtt aattgttccc aagagagcat ctgtcagttg
     2581 ttggcaaaat gatagacatt tgaaaatctg tcttctgaca aataaaaagc atctatgttc
     2641 actgcaatga tgttttaaat tatttgtctg tgtcatagaa gggtttatgc taagttttca
     2701 agatacaaag aagtgagggt tcaggtctga ccttggggaa ataaatgaat tacacttcaa
     2761 attgtgttgt cagctaagca gcagtagcca cagatcctat tgccatgccc taaacactca
     2821 gagaaaactt aattaaataa cttcgtatag gatactttat acgaagttat ccgccaccat
     2881 gcctgagccc gccaagtctg ctccagcccc taaaaagggt tctaagaagg ctgtgactaa
     2941 ggcgcagaag aaggatggta agaagcgtaa gcgcagccgc aaggagagct attctatcta
     3001 tgtgtataag gttctgaagc aggtccaccc cgacaccggc atctcatcca aggccatggg
     3061 gatcatgaac tccttcgtca acgacatctt cgagcgcatc gcgggcgagg cttctcgcct
     3121 ggctcactac aataagcgca gcaccatcac ctccagggag attcagacgg ctgtgcgcct
     3181 gctgctgcct ggggagctgg ctaagcatgc tgtgtccgag ggcactaagg cagttaccaa
     3241 gtacactagc gccaaggatc caccagtcgc caccatggtg agcaagggcg aggaggataa
     3301 catggccatc atcaaggagt tcatgcgctt caaggtccac atggagggct ccgtgaacgg
     3361 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3421 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     3481 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     3541 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     3601 ggtgaccgtg acccaggact cctccctcca ggacggcgag ttcatctaca aggtgaagct
     3661 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacga tgggctggga
     3721 ggcctcctcc gagcgcatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     3781 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     3841 gaagcccgtg cagctccccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     3901 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     3961 cggcatggac gagctgtaca agtaagtcga cagtacttcg actgtgcctt ctagttgcca
     4021 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactctcac
     4081 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4141 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4201 tgctggggat aacttcgtat agcatacatt atacgaagtt atccgccacc atgcctgagc
     4261 cagccaagag cgcaccagca ccaaagaaag ggtccaagaa agccgtcaca aaagcacaga
     4321 aaaaggatgg gaaaaagagg aagagaagcc ggaaagagtc ttacagtatc tacgtgtata
     4381 aggtcctgaa acaggtgcat cccgacactg gtatcagctc caaggcaatg ggcattatga
     4441 actctttcgt gaatgacatc tttgagagga ttgccggaga agctagtaga ctcgcacact
     4501 acaacaagag gagcaccatc accagcaggg agattcagac tgccgtgcgg ctgctcctgc
     4561 caggagagct ggcaaagcat gccgtgtcag aagggactaa ggccgtcacc aaatatacca
     4621 gcgccaagga cccaccctcc ggagccacca tggtgagcaa gggcgaggag ctgttcaccg
     4681 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4741 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     4801 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
     4861 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4921 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4981 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5041 tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg
     5101 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     5161 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5221 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     5281 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     5341 ctctcggcat ggacgagctg tacaagtaac tagtatttaa atgaagttcc tattctctag
     5401 aaagtatagg aacttcctcg agcctaggaa ttcactcctc aggtgcaggc tgcctatcag
     5461 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     5521 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     5581 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     5641 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     5701 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     5761 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     5821 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     5881 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     5941 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgcgcctag ttattaatgc
     6001 ggccgctcgc gacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg
     6061 attatctttc tagggttaag cccggtcgac ccaggtggca cttttcgggg aaatgtgcgc
     6121 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6181 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6241 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6301 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6361 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6421 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6481 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6541 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6601 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6661 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6721 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6781 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6841 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6901 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6961 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7021 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7081 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7141 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7201 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7381 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7501 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7561 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7621 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7681 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7741 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7801 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7861 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7921 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7981 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8041 ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg gatccagaca tgataagata
     8101 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     8161 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttagctt
     8221 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     8281 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     8341 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     8401 tgactaattg agatgcggat ccgctggcac gacaggtttc ccgactggaa agcgggcagt
     8461 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8521 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8581 agctatgacc atgattacgc caagctcgat ttaaccctag aaagatagtc tgcgtaaaat
     8641 tgacgcatgc atcctgcagg tagttcataa tagtaatcaa ttacggggtc attagttcat
     8701 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     8761 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     8821 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     8881 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     8941 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     9001 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     9061 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     9121 gcgatggggg cggggggggg gggggsgcgc gccaggcggg gcggggsggg gcgrggggcg
     9181 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     9241 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     9301 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     9361 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     9421 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     9481 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     9541 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     9601 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     9661 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     9721 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     9781 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     9841 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     9901 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     9961 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
    10021 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
    10081 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
    10141 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
    10201 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
    10261 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
    10321 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctagctagc
    10381 ataacttcgt atagtatacc ttatacgaag ttatctcgag ataacttcgt ataggatact
    10441 ttatacgaag ttatactgtc ataacttcgt atagcataca ttatacgaag ttatcccgg
//
