LOCUS       sequence-31181-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T
  TITLE     Activation of p53-dependent growth suppression in human cells by 
            mutations in PTEN or PIK3CA.
  JOURNAL   Mol Cell Biol. 2007 Jan . 27(2):662-77.
  PUBMED    17060456
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Jan . 27(2):662-77."
COMMENT     Sequence Label: pLP-LNCX-PIK3CA-W11STOP
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(62..95)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccaagcttc
       61 gataacttcg tatagcatac attatacgaa gttatcagtc gacccaccat ggattacaag
      121 gatgacgacg ataagcctcc aagaccatca tcaggtgaac tgtgaggcat ccacttgatg
      181 cccccaagaa tcctagtaga atgtttacta ccaaatggaa tgatagtgac tttagaatgc
      241 ctccgtgagg ctacattaat aaccataaag catgaactat ttaaagaagc aagaaaatac
      301 cccctccatc aacttcttca agatgaatct tcttacattt tcgtaagtgt tactcaagaa
      361 gcagaaaggg aagaattttt tgatgaaaca agacgacttt gtgaccttcg gctttttcaa
      421 ccctttttaa aagtaattga accagtaggc aaccgtgaag aaaagatcct caatcgagaa
      481 attggttttg ctatcggcat gccagtgtgt gaatttgata tggttaaaga tccagaagta
      541 caggacttcc gaagaaatgt tctgaacgtt tgtaaagaag ctgtggatct tagggacctc
      601 aattcacctc atagtagagc aatgtatgtc tatcctccaa atgtagaatc ttcaccagaa
      661 ttgccaaagc acatatataa taaattagat aaagggcaaa taatagtggt gatctgggta
      721 atagtttctc caaataatga caagcagaag tatactctga aaatcaacca tgactgtgta
      781 ccagaacaag taattgctga agcaatcagg aaaaaactcg aagtatgttg ctatcctctg
      841 aacaactaaa actctgtgtt ttannatatc agggcaagta tattttaaaa gtgtgtggat
      901 gtgatgaata cttcct
//
