LOCUS       sequence-311810-        9904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutive expression of 
            V5-tagged MX1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pLX302 MX1-V5 puro
FEATURES             Location/Qualifiers
     source          1..9904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        48..351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        352..555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     506..526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    578..602
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2589..2613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2615..2656
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2698..3286
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2751..2771)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3169..3180)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3358..3591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3669..3790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3706..3725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3760..3779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3830..3965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3985..4004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3986..4004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4014..4031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4014..4030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4023..4045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4172..4627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4259..4278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4469..4490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4653..4757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4758..4826,4827..5618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4976..4995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5789..6377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6278..6297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6531..6548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6701..6706,6707..6724,6725..6731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6739..6755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6744..6766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6763..6779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6763..6779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6798..6818
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6800..6818
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6846..7072
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7073..7253
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7300..7425
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7918..8151
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8336..8380
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8555..9055
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8974..8992
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9077..9676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9077..9096)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9573..9593
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9718..9835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
       61 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      121 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      181 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      241 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      301 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      361 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      421 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      481 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      541 tatataagca gagctctctg gctaactgtc gggatcaaca agtttgtaca aaaaagttgg
      601 catggttgtt tccgaagtgg acatcgcaaa agctgatcca gctgctgcat cccaccctct
      661 attactgaat ggagatgcta ctgtggccca gaaaaatcca ggctcggtgg ctgagaacaa
      721 cctgtgcagc cagtatgagg agaaggtgcg cccctgcatc gacctcattg actccctgcg
      781 ggctctaggt gtggagcagg acctggccct gccagccatc gccgtcatcg gggaccagag
      841 ctcgggcaag agctccgtgt tggaggcact gtcaggagtt gcccttccca gaggcagcgg
      901 gatcgtgacc agatgcccgc tggtgctgaa actgaagaaa cttgtgaacg aagataagtg
      961 gagaggcaag gtcagttacc aggactacga gattgagatt tcggatgctt cagaggtaga
     1021 aaaggaaatt aataaagccc agaatgccat cgccggggaa ggaatgggaa tcagtcatga
     1081 gctaatcacc ctggagatca gctcccgaga tgtcccggat ctgactctaa tagaccttcc
     1141 tggcataacc agagtggctg tgggcaatca gcctgctgac attgggtata agatcaagac
     1201 actcatcaag aagtacatcc agaggcagga gacaatcagc ctggtggtgg tccccagtaa
     1261 tgtggacatt gccaccacag aggctctcag catggcccag gaggtggacc ccgagggaga
     1321 caggaccatc ggaatcttga cgaagcctga tctggtggac aaaggaactg aagacaaggt
     1381 tgtggacgtg gtgcggaacc tcgtgttcca cctgaagaag ggttacatga ttgtcaagtg
     1441 ccggggccag caggagatcc aggaccagct gagcctgtcc gaagccctgc agagagagaa
     1501 gatcttcttt gagaaccacc catatttcag ggatctgctg gaggaaggaa aggccacggt
     1561 tccctgcctg gcagaaaaac ttaccagcga gctcatcaca catatctgta aatctctgcc
     1621 cctgttagaa aatcaaatca aggagactca ccagagaata acagaggagc tacaaaagta
     1681 tggtgtcgac ataccggaag acgaaaatga aaaaatgttc ttcctgatag ataaaattaa
     1741 tgcctttaat caggacatca ctgctctcat gcaaggagag gaaactgtag gggaggaaga
     1801 cattcggctg tttaccagac tccgacacga gttccacaaa tggagtacaa taattgaaaa
     1861 caattttcaa gaaggccata aaattttgag tagaaaaatc cagaaatttg aaaatcagta
     1921 tcgtggtaga gagctgccag gctttgtgaa ttacaggaca tttgagacaa tcgtgaaaca
     1981 gcaaatcaag gcactggaag agccggctgt ggatatgcta cacaccgtga cggatatggt
     2041 ccggcttgct ttcacagatg tttcgataaa aaattttgaa gagtttttta acctccacag
     2101 aaccgccaag tccaaaattg aagacattag agcagaacaa gagagagaag gtgagaagct
     2161 gatccgcctc cacttccaga tggaacagat tgtctactgc caggaccagg tatacagggg
     2221 tgcattgcag aaggtcagag agaaggagct ggaagaagaa aagaagaaga aatcctggga
     2281 ttttggggct ttccaatcca gctcggcaac agactcttcc atggaggaga tctttcagca
     2341 cctgatggcc tatcaccagg aggccagcaa gcgcatctcc agccacatcc ctttgatcat
     2401 ccagttcttc atgctccaga cgtacggcca gcagcttcag aaggccatgc tgcagctcct
     2461 gcaggacaag gacacctaca gctggctcct gaaggagcgg agcgacacca gcgacaagcg
     2521 gaagttcctg aaggagcggc ttgcacggct gacgcaggct cggcgccggc ttgcccagtc
     2581 ccccggttac ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct
     2641 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat
     2701 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2761 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2821 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2881 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2941 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3001 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3061 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3121 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3181 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3241 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3361 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3601 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3661 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3781 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3841 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3901 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3961 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4021 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4081 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4141 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4321 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4381 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4441 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4501 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4621 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6601 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6661 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6721 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6781 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6841 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     6901 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     6961 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7021 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7261 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7321 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7381 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7441 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7501 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7561 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7621 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7681 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7741 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7801 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7861 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     7921 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     7981 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8041 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8101 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8161 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8221 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8281 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8341 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8401 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8461 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8521 gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag gcagccctgg
     8581 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     8641 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     8701 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     8761 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     8821 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     8881 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     8941 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     9001 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagggggt
     9061 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9121 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9181 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     9241 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     9301 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9361 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9421 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9481 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9541 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9601 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9661 caagcccggt gcctgagaat tctagatctt gagacaaatg gcagtattca tccacaattt
     9721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9841 ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc ggggttatta
     9901 atag
//
