LOCUS       sequence-311811-        9013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutive expression of 
            V5-tagged OAS1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pLX302 OAS1-V5 puro
FEATURES             Location/Qualifiers
     source          1..9013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        356..559
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     510..530
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    582..606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1702..1726)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1728..1769
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1811..2399
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1864..1884)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2282..2293)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2471..2704
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2782..2903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2819..2838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2873..2892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2943..3078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3098..3117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3099..3117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3127..3144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3127..3143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3136..3158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3285..3740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3372..3391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3582..3603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3766..3870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3871..3939,3940..4731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4089..4108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4902..5490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5391..5410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5644..5661
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5778..5799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5814..5819,5820..5837,5838..5844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5852..5868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5857..5879
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5876..5892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5876..5892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5911..5931
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5913..5931
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5959..6185
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6186..6366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6413..6538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7031..7264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7449..7493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7668..8168
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8087..8105
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8190..8789
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8190..8209)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8686..8706
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8831..8948
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
      601 ttggcaccat gatggatctc agaaataccc cagccaaatc tctggacaag ttcattgaag
      661 actatctctt gccagacacg tgtttccgca tgcaaatcaa ccatgccatt gacatcatct
      721 gtgggttcct gaaggaaagg tgcttccgag gtagctccta ccctgtgtgt gtgtccaagg
      781 tggtaaaggg tggctcctca ggcaagggca ccaccctcag aggccgatct gacgctgacc
      841 tggttgtctt cctcagtcct ctcaccactt ttcaggatca gttaaatcgc cggggagagt
      901 tcatccagga aattaggaga cagctggaag cctgtcaaag cgagagagca ttttccgtga
      961 agtttgaggt ccaggctcca cgctggggca acccccgtgc gctcagcttc gtactgagtt
     1021 cgctccagct cggggagggg gtggagttcg atgtgctgcc tgcctttgat gccctgggtc
     1081 agttgactgg cagctataaa cctaaccccc aaatctatgt caagctcatc gaggagtgca
     1141 ccgacctgca gaaagagggc gagttctcca cctgcttcac agaactacag agagacttcc
     1201 tgaagcagcg ccccaccaag ctcaagagcc tcatccgcct agtcaagcac tggtaccaaa
     1261 attgtaagaa gaagcttggg aagctgccac ctcagtatgc cctggagctc ctgacggtct
     1321 atgcttggga gcgagggagc atgaaaacac atttcaacac agcccaggga tttcggacgg
     1381 tcttggaatt agtcataaac taccagcaac tctgcatcta ctggacaaag tattatgact
     1441 ttaaaaaccc cattattgaa aagtacctga gaaggcagct cacgaaaccc aggcctgtga
     1501 tcctggaccc ggcggaccct acaggaaact tgggtggtgg agacccaaag ggttggaggc
     1561 agctggcaca agaggctgag gcctggctga attacccatg ctttaagaat tgggatgggt
     1621 ccccagtgag ctcctggatt ctgctggtga gacctcctgc ttcctccctg ccattcatcc
     1681 ctgcccctct ccatgaagct ttgccaactt tcttgtacaa agtggttggt aagcctatcc
     1741 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
     1801 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1861 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1921 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     1981 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2041 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2101 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2161 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2221 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2281 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2341 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     2401 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     2461 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     2521 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2581 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2641 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2701 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     2761 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     2821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2881 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     2941 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3001 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3061 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     3121 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     3301 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3361 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3421 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3481 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3541 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3601 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3661 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3721 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5701 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5881 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     5941 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     6001 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     6061 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     6121 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     6181 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6241 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6301 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6361 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6421 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6481 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     6541 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     6601 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     6661 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     6721 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     6781 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     6841 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     6901 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6961 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     7021 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     7081 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     7141 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     7201 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     7261 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     7321 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7381 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7441 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7501 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7561 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7621 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccttg cgccttttcc
     7681 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
     7741 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     7801 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     7861 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     7921 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     7981 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     8041 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     8101 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     8161 ctccccaggg ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     8221 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     8281 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     8341 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     8401 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     8461 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     8521 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     8581 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     8641 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     8701 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8761 ggtgcatgac ccgcaagccc ggtgcctgag aattctagat cttgagacaa atggcagtat
     8821 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     8881 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     8941 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg
     9001 cccggggtta tta
//
