LOCUS       sequence-311812-        4173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to PP7 
            phage coat protein, as extracted from the oligo pool experiment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xPP7 oligo-library based
FEATURES             Location/Qualifiers
     source          1..4173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    128..176
                     /note="synthetic polyadenylation signal"
     misc_feature    190..281
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     230..249
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        348..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        652..855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     806..826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1419..1540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1430..1449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1484..1503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1793..1810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1964..2552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2044..2063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2723..3514,3515..3583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3346..3365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3584..3688)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3715..4170
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3802..3821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4012..4033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
       61 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
      121 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
      181 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
      241 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tggtacctag ttattaatag
      301 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      361 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      421 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      481 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      541 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      601 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      661 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      721 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      781 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      841 tatataagca gagctggttt atgaaccgtc agatccgagc tcctatctag cgtcgcgttg
      901 acgaattctt acatatggag tttatatgga aacccatacg cctgctggtc gtgaccagag
      961 atttcatatg ggaaactctg cgagacaatg ctaccttacc gtcggcccac ttgtttttac
     1021 ccatgattcc agtttatatg gaaacggaat cgcctgctgg tcgtgactaa ggagtttata
     1081 tggaaaccct tagaaaagcc gtcgccttga agccgagaac aaaaggagat tatatgaaat
     1141 ccctttatgg attgggtctc caattcctgt ctgttacgag tcaatatggt gaccgtacat
     1201 caggcttcgc agtatgcaac gcttgcgtaa gcacattata aggaatggct tattcaccgc
     1261 tttcctaaga ttggagtaaa tatggttacc caatgtacta actcgcagtt tcgacattat
     1321 atggaatgcg aaacagaaac gtcacgtcct ggctacggag tttatatgga aacccgtagg
     1381 aagaattccc cctaagcttc cgcttcgagc agacatgata agatacattg atgagtttgg
     1441 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1501 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1561 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1621 caaatgtggt aaaatcgata aggatcgagt atgtcgaccg atgcccttga gagccttcaa
     1681 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     1741 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
     1801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     3721 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3781 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3841 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3901 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3961 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4021 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4081 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4141 attttaacaa aatattaacg cttacaattt gcc
//
