LOCUS       sequence-311814-        4134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to QB 
            phage coat protein, as extracted from the oligo pool experiment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xQb oligo-library based
FEATURES             Location/Qualifiers
     source          1..4134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    128..176
                     /note="synthetic polyadenylation signal"
     misc_feature    190..281
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     230..249
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        348..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        652..855
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     806..826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1380..1501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1391..1410)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1445..1464
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1754..1771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1925..2513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2005..2024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2684..3475,3476..3544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3307..3326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3545..3649)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3676..4131
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3763..3782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3973..3994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
       61 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
      121 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
      181 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
      241 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tggtacctag ttattaatag
      301 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      361 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      421 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      481 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      541 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      601 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      661 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      721 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      781 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      841 tatataagca gagctggttt atgaaccgtc agatccgagc tcctatctag cgtcgcgttg
      901 acgaattctt acaatgcatg tctaagacag catctcgtgc tgatcgtgac ttggatgtcc
      961 aagacaccaa cgagacaatg ctaccttacc gtcggcccac ttgtttttac ccatgacatg
     1021 acgagatact cgcatgtcgc ctgctggtcg tgactaaggt gtttaaggtt aaacccttag
     1081 aaacagccgt cgccttgaag ccgagaacat tgcatgtcga agacagcaaa tggattgggt
     1141 ctccaattcc tgtctgtatg catgtctaag acagcatcat caggcttcgc agtatgcaac
     1201 gcttgcgatg cattgcaaag caagcatttc accgctttcc taagaaggat agtaatgact
     1261 accttgtact aactcgcaga tcgaactcta agagtcgatc agaaacgtca cgtcctggca
     1321 tgcatgtcta agacagcatg gaagaattcc ccctaagctt ccgcttcgag cagacatgat
     1381 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     1441 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     1501 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     1561 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcgag tatgtcgacc
     1621 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     1681 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     1741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2641 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2701 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2761 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2821 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2881 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2941 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3001 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3061 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3121 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3181 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3241 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3301 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3361 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3421 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3481 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3541 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3601 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3661 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3721 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3781 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3841 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3901 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3961 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4021 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4081 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tgcc
//
