LOCUS       sequence-311826-       12763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12763)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 12763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: RCASBP(A)-3xFlag-mHoxd13wt
FEATURES             Location/Qualifiers
     source          1..12763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..75
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        1242..1503
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2093..2115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2104..2120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2128..2134,2135..2152,2153..2158))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(2201..2219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2287..2391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2392..2460,2461..3252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2610..2629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3423..4011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3912..3931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4165..4182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4197..4337
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4263..4282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4423..4445
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4439..4630)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        5473..5734
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gccaccatgg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
       61 gatgacgatg acaagggatc catgagccgc tcggggactt gggacatgga tgggctgcgg
      121 gcggacggcg gggccgctgg ggcggcaccg gcctcctcct cctcctccgt agcagcgcct
      181 ggccagtgtc gcggcttcct gtcggcgccg gtgttcgcgg ggacacattc cggacgcgcg
      241 gcggcggcgg ccgcagcagc ggcggcggca gcggcggcag catccagctt cgcttaccca
      301 gggacctctg agcgcacagg ctcttcgtcg tcgtcgtcat cctcggctgt gatcgccact
      361 cgccccgagg ctcctgtggc caaagagtgt cccgcgccag cggccgccgc gaccgctgca
      421 gcaccgccgg gcgctcccgc gctgggctat ggctaccact tcggcaacgg ttactacagc
      481 tgccgcatgt cgcacggcgt aggcttacag cagaacgctc tcaagtcgtc cccgcacgct
      541 tctctcggag gtttcccggt ggagaagtac atggacgtgt cgggcctggc gagcagcagc
      601 gtaccgacca acgaggtgcc cgcaagagcc aaggaagtgt ccttctacca gggctacaca
      661 agtccctatc agcacgtgcc tggctacatc gacatggtgt ccacttttgg atccggggaa
      721 cctcggcacg aggcatacat ctccatggag ggctaccagt cctggacgct agccaacggg
      781 tggaacagcc aggtgtactg tgccaaggat cagccacagg ggtcccattt ttggaaatca
      841 tcctttccag gagatgtggc tttaaaccag ccggacatgt gcgtctaccg acggggaagg
      901 aagaaaaggg tgccttacac caaactgcag ctcaaagaac tggagaatga gtatgccatt
      961 aacaagttca ttaacaagga caagcggcgg cggatctcag ctgccacgaa cctttcggag
     1021 agacaagtaa ccatttggtt tcagaatcgg agggtgaagg acaagaaaat cgtctccaag
     1081 ctcaaagaca ctgtctcctg aaaatcgatg cgatgtacgg gccagatata cgcgtatctg
     1141 aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc
     1201 cctcaggata tagtagtttc gcttttgcat agggaggggg aaatgtagtc ttatgcaata
     1261 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     1321 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     1381 caacagacgg gtctgacatg gattggacga accactgaat tccgcattgc agagatattg
     1441 tatttaagtg cctagctcga tacaataaac gccatttgac cattcaccac attggtgtgc
     1501 acctgggttg atggccggac cgttgattcc ctgacgacta cgagcacctg catgaagcag
     1561 aaggcttcat ttggtgaccc cgacgtgata gttagggaat agtggtcggc cacagacggc
     1621 gtggcgatcc tgtctccatc cgtctcgtct atcgggaggc gagttcgatg accctggtgg
     1681 agggggctgc ggcttaggga ggcagaagct gagtaccgtc ggaggggatc cacaggacgg
     1741 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
     1801 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
     1861 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
     1921 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     1981 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     2041 agcaatttaa ctgtgataaa ctaccgcatt aaagctccaa gcttggctgt ttcctgtgtg
     2101 aaattgttat ccgctcacaa ttccacacat tatacgagcc ggaagcataa agtgtaaagc
     2161 ctggggtgcc taatgagtga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     2221 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2281 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2341 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2401 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2461 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2521 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2581 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     2641 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2701 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2761 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2821 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2881 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     2941 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3001 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3061 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3121 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3181 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3241 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3301 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3541 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4081 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4141 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4201 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4261 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     4321 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4381 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4441 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     4501 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     4561 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     4621 gtttggtcac tgatgcctcc gtgtaaggag gatttctgtt catgggggta atgataccga
     4681 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     4741 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     4801 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     4861 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     4921 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     4981 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     5041 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     5101 aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     5161 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     5221 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     5281 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgat
     5341 gcgatgtacg ggccagatat acgcgtatct gaggggacta gggtgtgttt aggcgaaaag
     5401 cggggcttcg gttgtacgcg gttaggagtc ccctcaggat atagtagttt cgcttttgca
     5461 tagggagggg gaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     5521 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     5581 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     5641 aaccactgaa ttccgcattg cagagatatt gtatttaagt gcctagctcg atacaataaa
     5701 cgccatttga ccattcacca cattggtgtg cacctgggtt gatggccgga ccgttgattc
     5761 cctgacgact acgagcacct gcatgaagca gaaggcttca tttggtgacc ccgacgtgat
     5821 agttagggaa tagtggtcgg ccacagacgg cgtggcgatc ctgtctccat ccgtctcgtc
     5881 tatcgggagg cgagttcgat gaccctggtg gagggggctg cggcttaggg aggcagaagc
     5941 tgagtaccgt cggagggagc tccagggccc ggagcgactg acccctgccg agaactcaga
     6001 gggtcgtcgg aagacggaga gtgagcccga cgaccacccc aggcacgtct ttggtcggcc
     6061 tgcggatcaa gcatggaagc cgtcattaag gtgatttcgt ccgcgtgtaa aacctattgc
     6121 gggaaaatct ctccttctaa gaaggaaata ggggccatgt tgtccctgtt acaaaaggaa
     6181 gggttgctta tgtctccctc agatttatat tctccggggt cctgggatcc catcactgcg
     6241 gcgctctccc agcgggcaat ggtacttgga aaatcgggag agttaaaaac ctggggattg
     6301 gttttggggg cattgaaggc ggctcgagag gaacaggtta catctgagca agcaaagttt
     6361 tggttgggat tagggggagg gagggtctct cccccaggtc cggagtgcat cgagaaacca
     6421 gctacggagc ggcgaatcga caaaggggag gaggtgggag aaacaactgt gcagcgagat
     6481 gcgaagatgg cgccagagga agcggccaca cctaaaaccg ttggcacatc ctgctatcat
     6541 tgcggaacag ctgttggctg caattgcgcc accgccacag cctcggcccc tcctccccct
     6601 tatgtgggga gtggtttgta tccttccctg gcgggggtgg gagagcagca gggccaggga
     6661 gataacacgt ctcggggggc ggagcagcca agggaggagc cagggcacgc gggtcaggcc
     6721 cctgggccgg ccctgactga ctgggcaagg gtaagggagg agcttgcgag tactggtccg
     6781 cccgtggtgg ccatgcctgt agtgattaag acagagggac ccgcctggac ccctctggag
     6841 ccaaaattga tcacaagact ggctgatacg gtcaggacca agggcttacg atccccgatc
     6901 actatggcag aagtggaagc gctcatgtcc tccccgttgc tgccgcatga cgtcacgaat
     6961 ctaatgagag tgattttagg acctgcccca tatgccttat ggatggacgc ttggggagtc
     7021 caactccaga cggttatagc ggcagccact cgcgaccccc gacacccagc gaacggtcaa
     7081 gggcgggggg aacggactaa cttggatcga ttaaagggct tagctgatgg gatggtgggc
     7141 aacccacagg gtcaggccgc attattaaga ccgggggaat tggttgctat tacggcgtcg
     7201 gctctccagg cgtttagaga agttgcccgg ctggcggaac ctgcaggtcc atgggcggac
     7261 atcacgcagg gaccatctga gtcctttgtt gattttgcca atcggcttat aaaggcggtt
     7321 gaggggtcag atctcccgcc ttccgcgcgg gctccggtga tcattgactg ctttaggcag
     7381 aagtcacagc cagatattca gcagcttata cgggcagcac cctccacgct gaccacccca
     7441 ggagagataa tcaaatatgt gctagacagg cagaagattg cccctcttac ggatcaaggc
     7501 atagccgcgg ccatgtcgtc tgctatccag cccttagtta tggcagtagt caatagagag
     7561 agggatggac aaactgggtc gggtggtcgt gcccgagggc tctgctacac ttgtggatcc
     7621 ccgggacatt atcaggcaca gtgcccgaaa aaacgaaagt caggaaacag ccgtgagcga
     7681 tgtcagctgt gtgacgggat gggacacaac gctaaacagt gtaggaagcg ggatggcaac
     7741 cagggccaac gcccaggaag aggtctctct tcggggccgt ggcccggccc tgagcagcct
     7801 gccgtctcgt tagcgatgac aatggaacat aaagatcgcc ccttggttag ggtcattctg
     7861 actaacactg ggagtcatcc agtcaaacaa cgttcggtgt atatcaccgc gctgttggac
     7921 tccggagcgg acatcactat tatttcggag gaggattggc ctactgattg gccggtggtg
     7981 gacaccgcga acccacagat ccatggcata ggagggggaa ttcccatgcg aaaatcccgg
     8041 gatatgatag aggtgggggt tattaaccga gacgggtcgt tggagcgacc cctgctcctc
     8101 ttccccgcag tcgctatggt tagagggagt atcctaggaa gagattgtct gcagggccta
     8161 gggctccgct tgacaaattt atagggaggg ccactgttct tactgttgcg ctacatctgg
     8221 ctattccgct caaatggaag tcagaccgca cgcctgtgtg gattgaccag tggccccttc
     8281 ctgaaggtaa acttgtagcg ctaacgcaat tagtggaaaa agaattacag ttaggacata
     8341 tagagccctc acttagttgt tggaacacac ctgtttttgt gatccggaag gcttccgggt
     8401 cttatcgctt attgcatgat ttgcgcgctg ttaacgccaa gcttgttcct tttggggccg
     8461 tccaacaggg ggcgccagtt ctctccgcgc tcccgcgtgg ctggcccctg atggtcctag
     8521 acctcaagga ttgcttcttt tctatccctc ttgcggaaca agatcgcgaa gcttttgcat
     8581 ttacgctccc ctctgtgaat aaccaggccc ccgctcgaag attccaatgg aaggtcttgc
     8641 cccaagggat gacctgttct cccactatct gtcagttggt agtgggtcag gtgctcgagc
     8701 ccttgcgact caagcaccca gctctgcgca tgttgcatta tatggacgat cttttgctag
     8761 ccgcctcaag tcatgatggg ttggaagcgg cagggaagga ggttatcggt acattggaaa
     8821 gagccgggtt cactatttcg ccggataaga tccagaggga gcccggagta caatatcttg
     8881 ggtacaagtt aggcagtacg tatgtagcac ccgtaggctt ggtagcagaa cccaggatag
     8941 ccaccttgtg ggatgttcaa aagctggtgg ggtcacttca gtggcttcgc ccagcgttag
     9001 ggatcccgcc acgactgatg ggtccctttt atgagcagtt acgagggtca gatcctaacg
     9061 aggcgaggga atggaatcta gacatgaaaa tggcctggag agagatcgta cagcttagca
     9121 ctactgctgc cttggaacga tgggaccctg cccagcctct ggaaggagcg gtcgctagat
     9181 gtgaacaggg ggcaataggg gtcctgggac agggactgtc cacacaccca aggccatgtt
     9241 tgtggttatt ctccacccaa cccaccaagg cgtttactgc ttggttagaa gtgctcaccc
     9301 ttttgattac taagctacgc gcttcggcag tgcgaacctt tggcaaggag gttgatatcc
     9361 tcctgttgcc tgcatgcttc cgggaggacc ttccgctccc ggaggggatc ctgttagcac
     9421 ttagggggtt tgcaggaaaa atcaggagta gtgacacgcc atctattttt gacattgcgc
     9481 gtccactgca tgtttctctg aaagtgaggg ttaccgacca ccctgtgccg ggacccactg
     9541 tctttaccga cgcctcctca agcacccata aaggggtggt agtctggagg gagggcccaa
     9601 ggtgggagat aaaagaaata gttgatttgg gggcaagtgt acaacaactg gaggcacgcg
     9661 ctgtggccat ggcacttctg ctgtggccga caacgcccac taatgtagtg actgactctg
     9721 cgtttgttgc gaaaatgtta ctcaagatgg gacaggaggg agtcccgtct acagcggcag
     9781 cttttatttt agaggatgcg ttaagccaaa ggtcagccat ggccgccgtt ctccacgtgc
     9841 ggagtcattc tgaagtgcca gggtttttca cagaaggaaa tgacgtggca gatagccaag
     9901 ccacctttca agcgtatccc ttgagagagg ctaaagatct tcataccgct ctccatattg
     9961 gaccccgcgc gctatccaaa gcgtgtaata tatctatgca gcaggctagg gaggttgttc
    10021 agacctgccc gcattgtaat tcagcccctg cgttggaggc cggggtaaac cctaggggtt
    10081 tgggacccct acagatatgg cagacagact ttacgcttga gcctagaatg gctccccgtt
    10141 cctggctcgc tgttactgtg gacaccgcct catcagcgat agtcgtaact cagcatggcc
    10201 gtgttacatc ggttgctgca caacatcatt gggccacggc tatcgccgtt ttgggaagac
    10261 caaaggccat aaaaacagat aacgggtcct gcttcacgtc taaatccacg cgggagtggc
    10321 tcgcgagatg ggggatagca cacaccaccg ggattccggg aaattcccag ggtcaagcta
    10381 tggtagagcg ggccaaccgg ctcctgaaag ataagatccg tgtgcttgcg gaaggggacg
    10441 gctttatgaa aagaatcccc gccagcaaac agggggaact actagccaaa gcaatgtatg
    10501 ccctcaatca ctttgagcgt ggtgaaaaca caaaaacacc ggtacaaaaa cactggagac
    10561 ctaccgttct tacagaagga cccccggtta aaatacgaat agagacaggg gagtgggaaa
    10621 aaggatggaa cgtgctggtc tggggacgag gttatgccgc tgtgaaaaac agggacactg
    10681 ataaggttat ttgggtaccc tctcggaaag ttaaaccgga tgtcacccaa aaggatgagg
    10741 tgactaagaa agatgaggcg agccctcttt ttgcaggcat ttctgactgg ataccctggg
    10801 aagacgagca agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag
    10861 aagacaccct tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg
    10921 tgtgaggtta cgggggtaag agctgatgtc cacttactcg agcagccagg gaacctttgg
    10981 attacatggg ccaaccgtac aggccaaacg gatttttgcc tctctacaca gtcagccacc
    11041 tccccttttc aaacatgttt gataggtatc ccgtccccta tttccgaggg tgattttaag
    11101 ggatatgttt ctgatacaaa ttgcaccacc ttgggaactg atcggttagt ctcgtcagcc
    11161 gactttactg gcggacctga caacagtacc accctcactt atcggaaggt ctcatgcttg
    11221 ttgttaaagc tgaatgtctc tatgtgggat gagccacctg aactacagct gttaggttcc
    11281 cagtctctcc ctaacattac taatattgct cagatttccg gtataaccgg gggatgcgta
    11341 ggcttcagac cacaaggggt tccttggtat ctaggttggt ctagacagga ggccacgcgg
    11401 tttctcctta gacacccctc tttctctaaa tccacggaac cgtttacggt ggtgacagcg
    11461 gataggcaca atctttttat ggggagtgag tactgcggtg catatggcta cagattttgg
    11521 aacatgtata actgctcaca ggtggggcgg cagtaccgct gtggtaatgc gcgcagcccc
    11581 cgcccgggtc ttcctgaaat ccagtgtaca aggagaggag gcaaatgggt taatcaatca
    11641 caggaaatta atgagtcgga gccgttcagc tttacggtga actgtacagc tagtagtttg
    11701 ggtaatgcca gtgggtgttg cggaaaagca ggcacgattc tcccgggaaa gtgggtcgac
    11761 agcacacaag gtagtttcac caaaccaaaa gcgctaccac ccgcaatttt cctcatttgt
    11821 ggggatcgcg catggcaagg aattcccagt cgtccggtag ggggcccctg ctatttaggc
    11881 aagcttacca tgttagcacc taagcataca gatattctca aggtgcttgt caattcatcg
    11941 cggacaggta taagacgtaa acgaagcacc tcacacctgg atgatacatg ctcagatgaa
    12001 gtgcagcttt ggggtcctac agcaagaatc tttgcatcta tcctagcccc gggggtagca
    12061 gctgcgcaag ccttaagaga aattgagaga ctagcctgtt ggtccgttaa acaggctaac
    12121 ttgacaacat cactcctcgg ggacttattg gatgatgtca cgagtattcg acacgcggtc
    12181 ctgcagaacc gagcggctat tgacttcttg ctcctagctc acggccatgg ctgtgaggac
    12241 gttgccggaa tgtgctgttt caatttgagt gatcacagtg agtctataca gaagaagttc
    12301 cagctaatga aggaacatgt caataagatc ggcgtggaca gcgacccaat tggaagttgg
    12361 ctgcgaggac tattcggggg aataggagaa tgggccgttc atttgctgaa aggactgctt
    12421 ttggggcttg tagttatttt gttgctagta gtgtgcctgc cttgcctttt gcaaatcgta
    12481 tgcggtaaca tcagaaagat gattaataac tccatcagct accacacgga atataagaag
    12541 ctgcaaaagg cctgtgggca gcctgaaagc agaatagtat aaggcagtac atgggtggtg
    12601 gtatagcgct tgcgagtcca tcgaggaagg caggaaagac agctattggt aattgtgaaa
    12661 tacgcttttg tctgtgtgct gcaggagctg agctgactct gctggtggcc tcgcgtacca
    12721 ctgtggcatc gattctagag cggccgcacg tgcagccgat ttt
//
