LOCUS       sequence-311828-       12800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 
            3xFlag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 12800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: RCASBP(A)-3xFlag-mHoxd13+7Ala
FEATURES             Location/Qualifiers
     source          1..12800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..66
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        1255..1516
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2106..2128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2117..2133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2141..2147,2148..2165,2166..2171))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(2214..2232)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2300..2404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2405..2473,2474..3265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2623..2642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3436..4024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3925..3944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4178..4195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4210..4350
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4276..4295)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4436..4458
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4452..4643)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        5486..5747
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gactacaaag accatgacgg tgattataaa gatcatgaca ttgattacaa ggatgacgat
       61 gacaagtcca tgagccgctc ggggacttgg gacatggatg ggctgcgggc ggacggcggg
      121 gccgctgggg cggcaccggc ctcctcctcc tcctccgtag cagcgcctgg ccagtgtcgc
      181 ggcttcctgt cggcgccggt gttcgcgggg acacattccg gacgcgcggc ggcggcggcc
      241 gcagcagcgg cggcggcggc cgcagcagcg gcggcggcag cggcggcagc atccagcttc
      301 gcttacccag ggacctctga gcgcacaggc tcttcgtcgt cgtcgtcatc ctcggctgtg
      361 atcgccactc gccccgaggc tcctgtggcc aaagagtgtc ccgcgccagc ggccgccgcg
      421 accgctgcag caccgccggg cgctcccgcg ctgggctatg gctaccactt cggcaacggt
      481 tactacagct gccgcatgtc gcacggcgta ggcttacagc agaacgctct caagtcgtcc
      541 ccgcacgctt ctctcggagg tttcccggtg gagaagtaca tggacgtgtc gggcctggcg
      601 agcagcagcg taccgaccaa cgaggtgccc gcaagagcca aggaagtgtc cttctaccag
      661 ggctacacaa gtccctatca gcacgtgcct ggctacatcg acatggtgtc cacttttgga
      721 tccggggaac ctcggcacga ggcatacatc tccatggagg gctaccagtc ctggacgcta
      781 gccaacgggt ggaacagcca ggtgtactgt gccaaggatc agccacaggg gtcccatttt
      841 tggaaatcat cctttccagg agatgtggct ttaaaccagc cggacatgtg cgtctaccga
      901 cggggaagga agaaaagggt gccttacacc aaactgcagc tcaaagaact ggagaatgag
      961 tatgccatta acaagttcat taacaaggac aagcggcggc ggatctcagc tgccacgaac
     1021 ctttcggaga gacaagtaac catttggttt cagaatcgga gggtgaagga caagaaaatc
     1081 gtctccaagc tcaaagacac tgtctcctga actagttacg atgcgatgta cgggccagat
     1141 atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa agcggggctt cggttgtacg
     1201 cggttaggag tcccctcagg atatagtagt ttcgcttttg catagggagg gggaaatgta
     1261 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     1321 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     1381 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattccgcat
     1441 tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgccattt gaccattcac
     1501 cacattggtg tgcacctggg ttgatggccg gaccgttgat tccctgacga ctacgagcac
     1561 ctgcatgaag cagaaggctt catttggtga ccccgacgtg atagttaggg aatagtggtc
     1621 ggccacagac ggcgtggcga tcctgtctcc atccgtctcg tctatcggga ggcgagttcg
     1681 atgaccctgg tggagggggc tgcggcttag ggaggcagaa gctgagtacc gtcggagggg
     1741 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
     1801 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
     1861 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
     1921 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     1981 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
     2041 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctc caagcttggc
     2101 tgtttcctgt gtgaaattgt tatccgctca caattccaca cattatacga gccggaagca
     2161 taaagtgtaa agcctggggt gcctaatgag tgagaattct tgaagacgaa agggcctcgt
     2221 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2401 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2461 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2521 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2581 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2641 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2701 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2761 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2821 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2881 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2941 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3001 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3061 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3541 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4201 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4261 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     4321 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     4381 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4441 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     4501 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     4561 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     4621 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag gaggatttct gttcatgggg
     4681 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     4741 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     4801 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     4861 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     4921 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     4981 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     5041 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     5101 cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg
     5161 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     5221 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     5281 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     5341 atagggcggc gatgcgatgt acgggccaga tatacgcgta tctgagggga ctagggtgtg
     5401 tttaggcgaa aagcggggct tcggttgtac gcggttagga gtcccctcag gatatagtag
     5461 tttcgctttt gcatagggag ggggaaatgt agtcttatgc aatactcttg tagtcttgca
     5521 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     5581 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     5641 catggattgg acgaaccact gaattccgca ttgcagagat attgtattta agtgcctagc
     5701 tcgatacaat aaacgccatt tgaccattca ccacattggt gtgcacctgg gttgatggcc
     5761 ggaccgttga ttccctgacg actacgagca cctgcatgaa gcagaaggct tcatttggtg
     5821 accccgacgt gatagttagg gaatagtggt cggccacaga cggcgtggcg atcctgtctc
     5881 catccgtctc gtctatcggg aggcgagttc gatgaccctg gtggaggggg ctgcggctta
     5941 gggaggcaga agctgagtac cgtcggaggg agctccaggg cccggagcga ctgacccctg
     6001 ccgagaactc agagggtcgt cggaagacgg agagtgagcc cgacgaccac cccaggcacg
     6061 tctttggtcg gcctgcggat caagcatgga agccgtcatt aaggtgattt cgtccgcgtg
     6121 taaaacctat tgcgggaaaa tctctccttc taagaaggaa ataggggcca tgttgtccct
     6181 gttacaaaag gaagggttgc ttatgtctcc ctcagattta tattctccgg ggtcctggga
     6241 tcccatcact gcggcgctct cccagcgggc aatggtactt ggaaaatcgg gagagttaaa
     6301 aacctgggga ttggttttgg gggcattgaa ggcggctcga gaggaacagg ttacatctga
     6361 gcaagcaaag ttttggttgg gattaggggg agggagggtc tctcccccag gtccggagtg
     6421 catcgagaaa ccagctacgg agcggcgaat cgacaaaggg gaggaggtgg gagaaacaac
     6481 tgtgcagcga gatgcgaaga tggcgccaga ggaagcggcc acacctaaaa ccgttggcac
     6541 atcctgctat cattgcggaa cagctgttgg ctgcaattgc gccaccgcca cagcctcggc
     6601 ccctcctccc ccttatgtgg ggagtggttt gtatccttcc ctggcggggg tgggagagca
     6661 gcagggccag ggagataaca cgtctcgggg ggcggagcag ccaagggagg agccagggca
     6721 cgcgggtcag gcccctgggc cggccctgac tgactgggca agggtaaggg aggagcttgc
     6781 gagtactggt ccgcccgtgg tggccatgcc tgtagtgatt aagacagagg gacccgcctg
     6841 gacccctctg gagccaaaat tgatcacaag actggctgat acggtcagga ccaagggctt
     6901 acgatccccg atcactatgg cagaagtgga agcgctcatg tcctccccgt tgctgccgca
     6961 tgacgtcacg aatctaatga gagtgatttt aggacctgcc ccatatgcct tatggatgga
     7021 cgcttgggga gtccaactcc agacggttat agcggcagcc actcgcgacc cccgacaccc
     7081 agcgaacggt caagggcggg gggaacggac taacttggat cgattaaagg gcttagctga
     7141 tgggatggtg ggcaacccac agggtcaggc cgcattatta agaccggggg aattggttgc
     7201 tattacggcg tcggctctcc aggcgtttag agaagttgcc cggctggcgg aacctgcagg
     7261 tccatgggcg gacatcacgc agggaccatc tgagtccttt gttgattttg ccaatcggct
     7321 tataaaggcg gttgaggggt cagatctccc gccttccgcg cgggctccgg tgatcattga
     7381 ctgctttagg cagaagtcac agccagatat tcagcagctt atacgggcag caccctccac
     7441 gctgaccacc ccaggagaga taatcaaata tgtgctagac aggcagaaga ttgcccctct
     7501 tacggatcaa ggcatagccg cggccatgtc gtctgctatc cagcccttag ttatggcagt
     7561 agtcaataga gagagggatg gacaaactgg gtcgggtggt cgtgcccgag ggctctgcta
     7621 cacttgtgga tccccgggac attatcaggc acagtgcccg aaaaaacgaa agtcaggaaa
     7681 cagccgtgag cgatgtcagc tgtgtgacgg gatgggacac aacgctaaac agtgtaggaa
     7741 gcgggatggc aaccagggcc aacgcccagg aagaggtctc tcttcggggc cgtggcccgg
     7801 ccctgagcag cctgccgtct cgttagcgat gacaatggaa cataaagatc gccccttggt
     7861 tagggtcatt ctgactaaca ctgggagtca tccagtcaaa caacgttcgg tgtatatcac
     7921 cgcgctgttg gactccggag cggacatcac tattatttcg gaggaggatt ggcctactga
     7981 ttggccggtg gtggacaccg cgaacccaca gatccatggc ataggagggg gaattcccat
     8041 gcgaaaatcc cgggatatga tagaggtggg ggttattaac cgagacgggt cgttggagcg
     8101 acccctgctc ctcttccccg cagtcgctat ggttagaggg agtatcctag gaagagattg
     8161 tctgcagggc ctagggctcc gcttgacaaa tttataggga gggccactgt tcttactgtt
     8221 gcgctacatc tggctattcc gctcaaatgg aagtcagacc gcacgcctgt gtggattgac
     8281 cagtggcccc ttcctgaagg taaacttgta gcgctaacgc aattagtgga aaaagaatta
     8341 cagttaggac atatagagcc ctcacttagt tgttggaaca cacctgtttt tgtgatccgg
     8401 aaggcttccg ggtcttatcg cttattgcat gatttgcgcg ctgttaacgc caagcttgtt
     8461 ccttttgggg ccgtccaaca gggggcgcca gttctctccg cgctcccgcg tggctggccc
     8521 ctgatggtcc tagacctcaa ggattgcttc ttttctatcc ctcttgcgga acaagatcgc
     8581 gaagcttttg catttacgct cccctctgtg aataaccagg cccccgctcg aagattccaa
     8641 tggaaggtct tgccccaagg gatgacctgt tctcccacta tctgtcagtt ggtagtgggt
     8701 caggtgctcg agcccttgcg actcaagcac ccagctctgc gcatgttgca ttatatggac
     8761 gatcttttgc tagccgcctc aagtcatgat gggttggaag cggcagggaa ggaggttatc
     8821 ggtacattgg aaagagccgg gttcactatt tcgccggata agatccagag ggagcccgga
     8881 gtacaatatc ttgggtacaa gttaggcagt acgtatgtag cacccgtagg cttggtagca
     8941 gaacccagga tagccacctt gtgggatgtt caaaagctgg tggggtcact tcagtggctt
     9001 cgcccagcgt tagggatccc gccacgactg atgggtccct tttatgagca gttacgaggg
     9061 tcagatccta acgaggcgag ggaatggaat ctagacatga aaatggcctg gagagagatc
     9121 gtacagctta gcactactgc tgccttggaa cgatgggacc ctgcccagcc tctggaagga
     9181 gcggtcgcta gatgtgaaca gggggcaata ggggtcctgg gacagggact gtccacacac
     9241 ccaaggccat gtttgtggtt attctccacc caacccacca aggcgtttac tgcttggtta
     9301 gaagtgctca cccttttgat tactaagcta cgcgcttcgg cagtgcgaac ctttggcaag
     9361 gaggttgata tcctcctgtt gcctgcatgc ttccgggagg accttccgct cccggagggg
     9421 atcctgttag cacttagggg gtttgcagga aaaatcagga gtagtgacac gccatctatt
     9481 tttgacattg cgcgtccact gcatgtttct ctgaaagtga gggttaccga ccaccctgtg
     9541 ccgggaccca ctgtctttac cgacgcctcc tcaagcaccc ataaaggggt ggtagtctgg
     9601 agggagggcc caaggtggga gataaaagaa atagttgatt tgggggcaag tgtacaacaa
     9661 ctggaggcac gcgctgtggc catggcactt ctgctgtggc cgacaacgcc cactaatgta
     9721 gtgactgact ctgcgtttgt tgcgaaaatg ttactcaaga tgggacagga gggagtcccg
     9781 tctacagcgg cagcttttat tttagaggat gcgttaagcc aaaggtcagc catggccgcc
     9841 gttctccacg tgcggagtca ttctgaagtg ccagggtttt tcacagaagg aaatgacgtg
     9901 gcagatagcc aagccacctt tcaagcgtat cccttgagag aggctaaaga tcttcatacc
     9961 gctctccata ttggaccccg cgcgctatcc aaagcgtgta atatatctat gcagcaggct
    10021 agggaggttg ttcagacctg cccgcattgt aattcagccc ctgcgttgga ggccggggta
    10081 aaccctaggg gtttgggacc cctacagata tggcagacag actttacgct tgagcctaga
    10141 atggctcccc gttcctggct cgctgttact gtggacaccg cctcatcagc gatagtcgta
    10201 actcagcatg gccgtgttac atcggttgct gcacaacatc attgggccac ggctatcgcc
    10261 gttttgggaa gaccaaaggc cataaaaaca gataacgggt cctgcttcac gtctaaatcc
    10321 acgcgggagt ggctcgcgag atgggggata gcacacacca ccgggattcc gggaaattcc
    10381 cagggtcaag ctatggtaga gcgggccaac cggctcctga aagataagat ccgtgtgctt
    10441 gcggaagggg acggctttat gaaaagaatc cccgccagca aacaggggga actactagcc
    10501 aaagcaatgt atgccctcaa tcactttgag cgtggtgaaa acacaaaaac accggtacaa
    10561 aaacactgga gacctaccgt tcttacagaa ggacccccgg ttaaaatacg aatagagaca
    10621 ggggagtggg aaaaaggatg gaacgtgctg gtctggggac gaggttatgc cgctgtgaaa
    10681 aacagggaca ctgataaggt tatttgggta ccctctcgga aagttaaacc ggatgtcacc
    10741 caaaaggatg aggtgactaa gaaagatgag gcgagccctc tttttgcagg catttctgac
    10801 tggataccct gggaagacga gcaagaagga ctccaaggag aaaccgctag caacaagcaa
    10861 gaaagacccg gagaagacac ccttgctgcc aacgagagtt aattatattc tcattattgg
    10921 tgtcctggtc ttgtgtgagg ttacgggggt aagagctgat gtccacttac tcgagcagcc
    10981 agggaacctt tggattacat gggccaaccg tacaggccaa acggattttt gcctctctac
    11041 acagtcagcc acctcccctt ttcaaacatg tttgataggt atcccgtccc ctatttccga
    11101 gggtgatttt aagggatatg tttctgatac aaattgcacc accttgggaa ctgatcggtt
    11161 agtctcgtca gccgacttta ctggcggacc tgacaacagt accaccctca cttatcggaa
    11221 ggtctcatgc ttgttgttaa agctgaatgt ctctatgtgg gatgagccac ctgaactaca
    11281 gctgttaggt tcccagtctc tccctaacat tactaatatt gctcagattt ccggtataac
    11341 cgggggatgc gtaggcttca gaccacaagg ggttccttgg tatctaggtt ggtctagaca
    11401 ggaggccacg cggtttctcc ttagacaccc ctctttctct aaatccacgg aaccgtttac
    11461 ggtggtgaca gcggataggc acaatctttt tatggggagt gagtactgcg gtgcatatgg
    11521 ctacagattt tggaacatgt ataactgctc acaggtgggg cggcagtacc gctgtggtaa
    11581 tgcgcgcagc ccccgcccgg gtcttcctga aatccagtgt acaaggagag gaggcaaatg
    11641 ggttaatcaa tcacaggaaa ttaatgagtc ggagccgttc agctttacgg tgaactgtac
    11701 agctagtagt ttgggtaatg ccagtgggtg ttgcggaaaa gcaggcacga ttctcccggg
    11761 aaagtgggtc gatagcacac aaggtagttt caccaaacca aaagcgctac cacccgcaat
    11821 tttcctcatt tgtggggatc gcgcatggca aggaattccc agtcgtccgg tagggggccc
    11881 ctgctattta ggcaagctta ccatgttagc acctaagcat acagatattc tcaaggtgct
    11941 tgtcaattca tcgcggacag gtataagacg taaacgaagc acctcacacc tggatgatac
    12001 atgctcagat gaagtgcagc tttggggtcc tacagcaaga atctttgcat ctatcctagc
    12061 cccgggggta gcagctgcgc aagccttaag agaaattgag agactagcct gttggtccgt
    12121 taaacaggct aacttgacaa catcactcct cggggactta ttggatgatg tcacgagtat
    12181 tcgacacgcg gtcctgcaga accgagcggc tattgacttc ttgctcctag ctcacggcca
    12241 tggctgtgag gacgttgccg gaatgtgctg tttcaatttg agtgatcaca gtgagtctat
    12301 acagaagaag ttccagctaa tgaaggaaca tgtcaataag atcggcgtgg acagcgaccc
    12361 aattggaagt tggctgcgag gactattcgg gggaatagga gaatgggccg ttcatttgct
    12421 gaaaggactg cttttggggc ttgtagttat tttgttgcta gtagtgtgcc tgccttgcct
    12481 tttgcaaatc gtatgcggta acatcagaaa gatgattaat aactccatca gctaccacac
    12541 ggaatataag aagctgcaaa aggcctgtgg gcagcctgaa agcagaatag tataaggcag
    12601 tacatgggtg gtggtatagc gcttgcgagt ccatcgagga aggcaggaaa gacagctatt
    12661 ggtaattgtg aaatacgctt ttgtctgtgt gctgcaggag ctgagctgac tctgctggtg
    12721 gcctcgcgta ccactgtggc atcgattcta gaccactgtg gccaggcggt agctgggacg
    12781 tgcagccgac caccatgtct
//
