LOCUS       sequence-31185-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CyP3
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(673..691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      687..734
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaatattttg tttactttag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcatg tcagttagaa taataacaaa ttacggagac
      121 ctaaaatttg agctattctg ttctcaatgc ccaaaagcct gcaaaaattt ccttgctctt
      181 tcagcaagtg gctactataa aaatacaatt tttcacaaga atataaaggg atttatcata
      241 caaggtggtg acccaactgg cacaggaaag ggcggagaga gtatctatgg gagatatttt
      301 gatgatgaga tttatccaga attgaagtat gataggagag gtattctttc gatggcaagc
      361 aaaggcgcta gtaaaaaacc aaatacaaac ggttcacagt tttttataac ttattcaagt
      421 ttaccgcaac ttaatggaga gtatgttatt tttggtaagc tgatagatgg ttttgaaact
      481 ctaaatacgt tagaaaactg tccatctgac aaatcccaca aaccaattga cgaaataatt
      541 attaaagata tagttattca ttcaaaccct attgctgacc aagaaatact tgattgatga
      601 cgaagcttgc ggccgcactc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      721 tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga ggcccggcag
      781 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
      841 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
      901 actaccgcat taaagcttat cgatgataag cctgtccaaa catg
//
