LOCUS       sequence-311855-        4911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pex22(1-36)-mRuby2 peroxisome marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
  TITLE     Peroxisome compartmentalization of a toxic enzyme improves alkaloid 
            production.
  JOURNAL   Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4.
  PUBMED    33046851
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4."
COMMENT     Sequence Label: pSJD001
FEATURES             Location/Qualifiers
     source          1..4911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             821..1528
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             complement(2506..3321)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2506..3321)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3229..3248
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3313..3332
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3546..4134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4035..4054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaggcaagcc cagaaaaata tcgcaagcac ctttggtctt acagtgccaa cttttggcct
       61 gccgacgtta agagtacaaa gctgatggca atgtacgaca agataacaga gtctcaaaag
      121 aagtgaaaca atttttcttc accacatttt ccattgttcc ttccccccat aactataaac
      181 gtatttatgt atatatattt gcgtgtaagt gtgtgtacta tagggcaccg taaagtaata
      241 atgcttaatt agttactact atgaccatat aagaggtcat actgtatgaa gccacaaagc
      301 agatagatca atcatgttta acgaaaactg ttaatcgaag attatttctt tttttttttc
      361 tctttccttt ttacaaagaa aatttttttt gcgctttttg ccatcaccat cgcaagttct
      421 gggacaattg ttctctttcg ctccagttcc aaggaaagag gtttctgttt tacttaatag
      481 aaagtgtcat cttgtatttt atatctcttc tttcttgtgt aaaattcttt agttttgatt
      541 ttgtattttt aggacagtga gctacgaagt aacattttta cttaataacc gtttgaagca
      601 tagagcaggc cctggtatca ccacctaata tctggctttt tattcaataa aaactcaaaa
      661 aaaaaaatcc aaaaaaaact aaaaaaccaa taaaaataaa agatctatgc caccaccatc
      721 aagaagtaga ataaacaaaa caagaacatt aggaatagtg ggtacagcta tagcagtgtt
      781 ggtcacgtcc tactatatat atcaaaaggt gacaggttct gtgtccaaag gagaggagtt
      841 aatcaaggaa aacatgagaa tgaaagttgt catggagggc tccgttaatg gtcaccaatt
      901 caagtgtaca ggggaaggtg aaggtaatcc ttacatgggt acacaaacta tgagaattaa
      961 agtaattgaa ggcggaccac taccatttgc atttgacatt ctggcaacgt cattcatgta
     1021 cggatcacga actttcatca agtaccctaa aggtatacca gactttttca agcaatcttt
     1081 tccagagggt tttacatggg aaagggttac aagatacgaa gatgggggtg tcgtcacagt
     1141 tatgcaagat acttcattag aagatggctg ccttgtctat catgtgcaag taagaggggt
     1201 gaattttcct tctaacggac ctgtgatgca gaaaaagacc aaaggttggg aaccaaatac
     1261 tgaaatgatg tacccagctg atggaggttt gagaggctac acacacatgg cgcttaaagt
     1321 tgatggtgga ggtcatttgt cttgtagttt tgttaccact tatcgttcta aaaagactgt
     1381 tggcaatatc aaaatgccag gaatacatgc tgtagaccac agactagaaa gactcgaaga
     1441 gagcgataac gaaatgttcg ttgtacagag agagcatgcc gtagccaaat ttgctggctt
     1501 aggcggtggt atggatgaat tgtataaggg atcctaactc gaggccaatt ggtgcggcaa
     1561 ttgataataa cgaaaatgtc ttttaatgat ctgggtataa tgaggaattt tccgaacgtt
     1621 tttactttat atatatatat acatgtaaca tatattctat acgctataga gaaaggaaat
     1681 ttttcaatta aaaaaaaaat agagaaagag tttcacttct tgattatcgc taacactaat
     1741 ggttgaagta ctgctacttt aattttatgc tggaaatctg ctcgtcagtg gtgctcacac
     1801 tgacgaatca tgtacagatc ataccgatga ctgcctggcg actcacaact aagcaagaca
     1861 gccggaacca gcgccggcga acaccactgc atatatggca tatcacaaca gtccacgtct
     1921 caagcagtta cagagatgtt acgaaccact agtgcactgc agtacatgac atgtatgggt
     1981 tgaaaatatt tagaggatgc taaaagtccc gtctaaaata gaggaagtca tggattacat
     2041 tatcaccttt attgtcctgt tccgtctggc taatttggga cgaggaacgt ttgtctccat
     2101 tcgcaacctt ttgaaaccat atgaacggcg gctaatctga cagatgtctt atatttctag
     2161 gattgtagaa agcctcctca tcaaaccacc ttgtttgaaa aaggcactag tgcggttgct
     2221 ttacttgatc tagtattctc ctaccttgga gcggtcccat atcaactaaa ttcagttttc
     2281 tgactgagtt aaaaactaat gtagcgacac cacttccatt ggtgcctccg ttattttttt
     2341 tttgtatgtt aatgctaaat aatacaccgc tatgtatttc agggcactac ttctacacat
     2401 caacggtact aaacatttcg ctcaagatcg gtccgctttc acgtatttgg atgctatgca
     2461 atgttgccga gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc
     2521 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     2581 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     2641 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     2701 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     2761 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     2821 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg
     2881 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     2941 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     3001 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     3061 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     3121 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     3181 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     3241 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt
     3301 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     3361 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     3421 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcagt catgaccaaa
     3481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4141 agcaacgcgg cctttttacg gttcctggcc ttttgctcac atgttctttc ctgcgttatc
     4201 ccctgattct gtggataacc gtgcggccgc caattgaaaa aggccactac tgagccaagc
     4261 gcagaaccag atgagccatt cattggatac ttgggtgacg ttactgcatc tataaaccaa
     4321 gatataaaag aatatggtcg cagaagaaca gtaataccta caagcgattt agtagcgtcg
     4381 ttctacacca aaaagacgcc tgtttttaag gtcaagttca gcagaagcaa tttagccctt
     4441 gcaggaggtg ctttcaggcc ataattcaca ctgaaaaaaa taacagcatg gaagtgatat
     4501 gtaataagta taacctaatc cgatatgtaa ctattagatg ctttttcgac atcgtacttg
     4561 acctgctcgg cggttatcat agcgtcttct ttagcctttt ttttttcgct cagtcatcgg
     4621 ggccaaaaca agaaaggcga agaacagaaa aatgaaaaat gtaactggtt gttgtaaaaa
     4681 ccttccagag aaataagatg aaatgtatgt tgctggcacg aacgaagaca aataccctga
     4741 attcgcatct agactgatga gacgtggtag agccacaaac agccggtaca agcaacgatc
     4801 tccaggacca tctgaatcat gcgcggatga cacgaactca cgacggcgat cacagacatt
     4861 aacccacagt acagacactg cgacaacgtg gcaattcgtc gcaatacaac g
//
