LOCUS       sequence-311857-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of the cytoplasmic isoform of rat 
            USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  TITLE     rat USP19 constructs
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HisFlag-rUSP19CYT
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     678..698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     772..791
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        772..790
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      897..906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             909..926
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             927..992
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5010..5027)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5016..5240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5286..5714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5373..5392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5583..5604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5723..5743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5728..6057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5908..6043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5970..5989
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6124..6918
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6178..6197)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6788..6807
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7094..7215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7131..7150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7185..7204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7264..7280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7264..7280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7277..7299)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7288..7304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7312..7318,7319..7336,7337..7342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7357..7378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7495..7512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7666..8254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7746..7765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8425..9216,9217..9285))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9048..9067
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9286..9390)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9465..9484)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
       61 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      121 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      541 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
      781 tcactatagg gagacccaag ctggctagtt aagcttggta ccgagctcgg atccactagt
      841 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagcca
      901 ccatggcaca tcatcatcat catcacgact acaaagacca tgacggtgat tataaagatc
      961 atgacatcga ttacaaggat gacgatgaca agcgcccggg ctgcaggaat tcgatgggtc
     1021 catgtgctgg caccagtgct acaggcccaa ggagggggcc tccgggattg gaggaggcaa
     1081 ctagtaagaa gaaacagaag gatcgagcaa accaggaaag taaggatgga gatcctagga
     1141 gagtgtccat gcctcgaaag gagcccacca aagatgaact gttgctcgat tggagacaga
     1201 gttcagataa ggtggttgtt aagctgcgcg tgggaacagg tcccatatgt ctggaggaag
     1261 tagatgctgc tttcacagac acggactgtg tggtgaggct tccagatggt cggcagtggg
     1321 gtggtgtctt ctttgctgaa atacaaagtt cttgtaccaa agtgcagact cacaagggtg
     1381 gtcttctaca gttggcacta ccaaagaagg tgcctcttct cacgtggccc tctctcctga
     1441 agaaacctct agggacccaa gaactggtgc caggattgcg gtgccaggag aacggacaag
     1501 aactgtctcc cattgccctg gagccaggtt ctgagccccg cagagctaaa caggaagctc
     1561 gaaaccagaa gagggcccag ggccgtggtg aggtaggctc aggggctagc cctggggcac
     1621 aggcagggcc cagtgccaag agggctgttc atctctgcag agggccagaa ggtgaagggt
     1681 ccatggatgg gcctggcccc cagggtgatg ccccatcctt cctgtccgac tcagctacgc
     1741 aggttgaggc tgaggagcag ctccatgttc caccagtgaa ccctcaaaca agtctcttgg
     1801 gctcagagaa gaatttagcc cttttgacag tagagaagac agtgtccccc aggagtgact
     1861 cagtctcccc agtcatgatc cggaacagag accctgagaa agatgaccat ttcaaagagg
     1921 agatggcagt tggagctgat cctgcagcct tagcggatga accagagtct atggtgaacc
     1981 tggcatttgt caagaatgat tcgtatgaga aggggccgga ttcagtggtg gtgcacgtgt
     2041 atgtgaagga gagccgcagg gatacctctc gagtactttt ccgagagcag gacttcacgc
     2101 tgatcttcca gaccagggat ggaaattttc tgaggctgca tccgggctgt gggccccaca
     2161 ccatcttccg ctggcaggtg aagctcagga acttgattga gccagagcaa tgtacgtttt
     2221 gtttcacggc ctctcgaatc gatatctgcc tccggaagcg gcagagtcag cgctgggggg
     2281 gactggaggc ccctgctaca cgagtgggtg gtgcaaaggt tgccgtgccg acaggtccaa
     2341 cccctttgga ttcaacccct ccaggaggtg gccccctccc tctgactggc caggaggaag
     2401 ccagggctgt agagaaggaa aaacccaagg ctcggtcaga ggactcgggg ctggatggtg
     2461 tggtggctcg cacccccttg gagcatgtta ccccaaagcc agaaccgcac ttggcctcgc
     2521 ccaaacccac atgtatggtg cctccaatgc ctcatagtcc agtgagtgga gatagtgtgg
     2581 aggaggatga agaggaagag aagaaggttt gtctcccagg ctttactggc ctcgtcaact
     2641 tagggaacac ctgcttcatg aatagtgtca ttcagtctct gtccaacact cgggagcttc
     2701 gtgacttctt ccacgaccga tcctttgagg ctgagattaa ctacaataac ccattgggga
     2761 ctggtgggcg tctggctatt ggcttcgctg tgctgctccg ggccctgtgg aagggcactc
     2821 accaagcctt tcaaccctcc aagctaaagg ccattgtagc aagcaaggcc agccagttta
     2881 caggctacgc acagcatgat gcccaagagt tcatggcttt cttgttggat gggctgcatg
     2941 aagacctgaa tcggatccaa aacaaaccct acacagagac tgtggactca gatgggcgac
     3001 ctgacgaggt ggtggctgaa gaagcatggc agcggcacaa gatgaggaac gactcattca
     3061 ttgtggacct gtttcagggc cagtacaagt caaagctggt gtgccctgtg tgtgccaagg
     3121 tctccatcac ttttgacccg ttcctttatc tgccggtacc cttgccacaa aagcaaaagg
     3181 ttctccccat atattatttt gccagagagc cccatagcaa acccatcaag ttcctggtga
     3241 gcgtcagcaa ggagaactcc agcgcaagcg aagttttgga gtccctctct cagagtgtcc
     3301 acgtgaagcc tgagagcctg cgcctggctg aggtaattaa gaatcgtttc caccgtgttt
     3361 tcttgccctc ccactcactg gatgctgtgt cgccaacgga cgtgctcctg tgctttgagc
     3421 tgctatcccc agaattggct aaggagaggg tagtggtgct agaggtgcag cagcgccccc
     3481 aggtacccag catccctatc tccaagtgtg cagcctgcca gcggaagcag caatcagaag
     3541 atgaaaaact gaagcgttgt acccgttgct accgtgtggg ctactgcaac cagttctgtc
     3601 agaaaaccca ttggcctgac cacaaaggcc tctgccgccc cgagaacatt ggctacccct
     3661 tcctggtcag tgtacctgct tcacgcctca cttatgcccg tcttgctcag ctactagaag
     3721 gttatgcccg gtactctgtg agcgtattcc aaccaccctt ccagcctggc cgaatggctt
     3781 tggaatcaca gagccctggc tgtaccacgt tgctttcaac cagctctctg gaggctgggg
     3841 atagtgaaag agaacccatt cagccttctg agctccagtt ggtgactcct gtggctgagg
     3901 gggataccgg ggctcaccgc atgtggccac ctgctgatcg gggtcctgtg cctagcacca
     3961 gtggaatttc ttccgagatg ctggccagtg ggcctatgga agggtgttcc ttgcttgctg
     4021 gtgagagggt gtctcggcct gaagctgctg tgcctgggta ccagcactca cgtgagtctg
     4081 tgagcgccca cacgccccag ttcttcatct ataaaattga tgcgtcaagc cgtgagcagc
     4141 ggctggagga caaaggggac actccactgg agctaggtga tgactgtagc ctggctctgg
     4201 tgtggcggaa caacgaacgc cttcaggagt ttgtgctggt ggcctccaag gagctggagt
     4261 gtgctgaaga cccaggctcc gctggtgagg ctgcccgtgc tggccacttc accctggacc
     4321 agtgcctcaa tctctttaca cggcctgaag tgctggcacc tgaggaagcc tggtactgcc
     4381 cacagtgcaa acagcatcga gaggcctcca agcagctgct gctgtggcgc ctaccaaatg
     4441 tgctcatcgt gcagctcaag cgtttctcct ttcgtagctt catttggcgt gacaagatca
     4501 atgacttggt ggagtttcca gttcggaacc tggacttgag caagttctgt attggtcaga
     4561 aagaggagca gctgcctagc tatgacctgt atgcagtcat caaccactac ggaggcatga
     4621 tcggcggcca ctacactgcc tgtgcacggc tgcccagtga ccgtagtagc cagcgcagtg
     4681 acgtgggctg gcgcttgttt gatgacagca cggtgacaac agtagacgag agccaggtgg
     4741 tgacacgcta tgcctatgtg ctcttctacc gtcggcggaa ctctcctgtg gagaggcccc
     4801 ccagggcagc tcacgctgaa caccacccag acctaggccc tgcagccgag gctgctgcca
     4861 gccagggact aggccctggc caggcccccg aggtggcccc cacgcggaca gcccctgaac
     4921 gattcgtccc ccctgtggac cgcccagccc ccacgtacag caacatggag gaggtcgatt
     4981 agcaggtccg tcgaaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5041 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5101 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5161 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5221 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5281 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5341 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5401 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5461 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5521 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5581 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5641 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5701 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5761 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5881 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5941 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6001 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6061 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     6121 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6181 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6241 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6301 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6361 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6421 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6481 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6541 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6601 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6661 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6721 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6781 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6841 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6901 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
     6961 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7021 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7081 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7201 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7261 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7321 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7381 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7621 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7681 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7741 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7801 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7861 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7921 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7981 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8041 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8101 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8161 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8221 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8281 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8341 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8401 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8461 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8521 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8581 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8641 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8701 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8761 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8821 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8881 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8941 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9001 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9061 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9121 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9181 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9241 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9301 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9361 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9421 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9481 gccgcatagt taagccagta tctgctccct gc
//
