LOCUS       sequence-31186-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CyP2
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(673..691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      687..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc aacccagttg tatactttga tatctcaatc
      121 ggccaaactc cagccggtcg tatcactatg gagttgtttg cagataaagt accaataaca
      181 gccgaaaact tcagagcact ctgcactgga gaaaagggaa tgggacagtc aggaaagccc
      241 ctttgctaca ctggatcatt cttccatcgt atcattccac aattcatgat ccaaggtggt
      301 gattttactc gtggtgatgg tactggtggt gagtcaatct atggctccaa gttcagagat
      361 gaaaacttcg tctacactca tgacgcccca ttccttctct ctatggctaa tgctggtcca
      421 aacactaatg gatcccaatt cttcattaca actgttccat gcccatggtt ggacggaaag
      481 catgttgtat ttggaaaggt tttggaagga atggaagtag taaagagcat tgagaagtgt
      541 ggttctcaaa atggaaagcc aaccaagtct gtatgcatca ctgcttctgg agtttgatga
      601 cgaagcttgc ggccgcactc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag cataacccct tgggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggatat cccgcaagag gcccggcagt
      781 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
      841 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
      901 ctaccgcatt aaagcttatc g
//
