LOCUS       sequence-311872-       17263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of human MALAT1 with deleted PARP1 binding
            sites. Contains CopGFP marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17263)
  TITLE     Jianjun Zhao Lab Plasmids
REFERENCE   2  (bases 1 to 17263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCDH-hMALAT1 deletion 11 PARP1 binding sites
FEATURES             Location/Qualifiers
     source          1..17263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2373..2584
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2597..2865
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        11405..11616
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             11629..11897
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      11926..11935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11956..12618
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             12688..12741
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             12742..13341
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12742..12761)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13238..13258
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13342..13930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13395..13415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13813..13824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14004..14237
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    14309..14430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14346..14365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14400..14419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      14470..14605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14638..14654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14638..14654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14651..14673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14662..14678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14686..14692,14693..14710,14711..14716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14731..14752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14869..14886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15040..15628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(15799..16590,16591..16659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16422..16441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16660..16764)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16832..16850
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16888..16910)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     17010..17029
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17223..17245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17237..17254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17238..17254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttgaaa gaccccacct gtaggtttgg caagttagct taagtaacgc
     1981 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2041 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2101 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagcg
     2161 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2221 ctaaccaatc agtttgcttc ttgcttctgt ttgtgtgctt ctgctccctg agctcaataa
     2281 aagagcccac aacccctcac ttggtgggcc agtcctctga tagactgtgt cccctggata
     2341 cccgtattct aggcggccgc gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2401 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     2461 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2521 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2581 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     2641 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     2701 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     2761 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     2821 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatcgcggc cgcgtaaagg
     2881 actggggccc cgcaactggc ctctcctgcc ctcttaagcg cagcgccatt ttagcaacgc
     2941 agaagcccgg cgccgggaag cctcagctcg cctgaaggca ggtcccctct gacgcctccg
     3001 ggagcccagg tttcccagag tccttgggac gcagcgacga gttgtgctgc tatcttagct
     3061 gtccttatag gctggccatt ccaggtggtg gtatttagat aaaaccactc aaactctgca
     3121 gtttggtctt ggggtttgga ggaaagcttt tatttttctt cctgctccgg ttcagaaggt
     3181 ctgaagctca tacctaaccg ggcataacac agaatctgca aaacaaaaac ccctaaaaaa
     3241 gcagacccag agcagtgtaa acacttctgg gtgtgtccct gactggctgc ccaaggtctc
     3301 tgtgtcttcg gagacaaagc cattcgctta gttggtctac tttaaaaggc cacttgaact
     3361 cgctttccat ggcgatttgc cttgtgagca ctttcaggag agcctggaag ctgaaaaacg
     3421 gtagaaaaat ttccgtgcgg gccgtggggg gctggcggca actggggggc cgcagatcag
     3481 agtgggccac tggcagccaa cggcccccgg ggctcaggcg gggagcagct ctgtggtgtg
     3541 ggattgaggc gttttccaag agtgggtttt cacgtttcta agatttccca agcagacagc
     3601 ccgtgctgct ccgatttctc gaacaaaaaa gcaaaacgtg tggctgtctt gggagcaagt
     3661 cgcaggactg caagcagttg ggggagaaag tccgccattt tgccacttct caaccgtccc
     3721 tgcaaggctg gggctcagtt gcgtaatgga aagtaaagcc ctgaactatc acactttaat
     3781 cttccttcaa aaggtggtaa actataccta ctgtccctca agagaacaca agtgctttaa
     3841 gaggtatttt aaaagttccg ggggttttgt gaggtgtttg atgacccgtt taaaatatga
     3901 tttccatgtt tcttttgtct aaagtttgca gctcaaatct ttccacacgc tagtaattta
     3961 agtatttctg catgtgtagt ttgcattcaa gttccataag ctgttaagaa aaatctagaa
     4021 aagtaaaact agaacctatt tttaaccgaa gaactacttt ttgcctccct cacaaaggcg
     4081 gcggaaggtg atcgaattcc ggtgatgcga gttgttctcc gtctataaat acgcctcgcc
     4141 cgagctgtgc ggtaggcatt gaggcagcca gcgcaggggc ttctgctgag ggggcaggcg
     4201 gagcttgagg aaaccgcaga tgagtttttt tctctttgaa agatagagat taatacaact
     4261 acttaaaaaa tatagtcaat aggttactaa gatattgctt agcgttaagt ttttaacgta
     4321 attttaatag cttaagattt taagagaaaa tatgaagact tgaaaagata aaaggtttct
     4381 aaaacatgac ggaggttgag atgaagcttc ttcatggagt aaaaaatgta tttaaaagaa
     4441 aattgagaga aaggactaca gagccccgaa ttaataccaa tagaagggca atgcttttag
     4501 attaaaatga aggtgactta aacagcttaa agtttagttt aaaagttgta ggtgattaaa
     4561 ataatttgaa ggcgatcttt taaaaagaga ttaaactgaa ggtgattaaa agaccttgaa
     4621 atccatgacg cagggagaat tgcgtcattt aaagcctagt taacgcattt actaaacgca
     4681 gacgaaaatg gaaagattaa ttgggagtgg taggatgaaa caatttggag aagatagaag
     4741 tttgaagtgg aaaactggaa gacagaagta cgggaaggcg aagaaaagaa tagagacata
     4801 cttttagaag aaaaaagata aatttaaacc tgaaaagtag gaagcagaag aaaaaagaca
     4861 agctaggaaa caaaaagcta agggcaaaat gtacaaactt agaagaaaat tggaagatag
     4921 aaacaagata gaaaatgaaa atattgtcaa gagtttcaga tagaaaatga aaaacaagct
     4981 aagacaagta ttggagaagt atagaagata gaaaaatata aagccaaaaa ttggataaaa
     5041 tagcactgaa aaaatgagga aattattggt aaccaattta ttttaaaagc ccatcaattt
     5101 aatttctggt ggtgcagaag ttagaaggta aagcttgaga agatgagggt gtttacgtag
     5161 accagaacca atttagaaga atacttgaag ctagaagggg aagttggtta aaaatcacat
     5221 caaaaagcta ctaaaaggac tggtgtaatt taaaaaaaac taaggcagaa ggcttttgga
     5281 agagttagaa gaatttggaa ggccttaaat atagtagctt agtttgaaaa atgtgaagga
     5341 ctttcgtaac ggaagtaatt caagatcaag agtaattacc aacttaatgt ttttgcattg
     5401 gactttgagt taagattatt ttttaaatcc tgaggactag cattaattga cagctgaccc
     5461 aggtgctaca cagaagtgga ttcagtgagt ctaggaagac agcagcagac aggattccag
     5521 gaaccagtgt ttgatgaagc taggactgag gagcaagcga gcaagcagca gttcgtggtg
     5581 aagataggaa aagagtccag gagccagtgc ggaagaagga aggagcgcta aaggattcca
     5641 ggaaggagcg agtgcaattt ggcttggcaa ccacacggag gaggcgagca ggcgttgtgc
     5701 gtagaggatc ctagaccagc atgccagtgt gccaaggcca cagggaaagc gagtggttgg
     5761 taaaaatccg tgaggtcggc aatatgttgt ttttctggaa cttacttatg gtaacctttt
     5821 atttattttc taatataatg ggggagtttc gtactgaggt gtaaagggat ttatatgggg
     5881 acgtaggccg atttccgggt gttgtaggtt tctctttttc aggcttatac tcatgaatct
     5941 tgtctgaagc ttttgagggc agactgccaa gtcctggaga aatagtagat ggcaagtttg
     6001 tgggtttttt tttttttaca cgaatttgag gaaaaccaaa tgaatttgat agccaaattg
     6061 agacaatttc agcaaatctg taagcagttt gtatgtttag ttggggtaat gaagtatttc
     6121 agttttgtga atagatgacc tgtttttact tcctcaccct gaattcgttt tgtaaatgta
     6181 gagtttggat gtgtaactga ggcggggggg agttttcagt attttttttt gtgggggtgg
     6241 gggcaaaata tgttttcagt tctttttccc ttaggtctgt ctagaatcct aaaggcaaat
     6301 gactcaaggt gtaacagaaa acaagaaaat ccaatatcag gataatcaga ccaccacagg
     6361 tttacagttt atagaaacta gagcagttct cacgttgagg tctgtggaag agatgtccat
     6421 tggagaaatg gctggtagtt actctttttt ccccccaccc ccttaatcag actttaaaag
     6481 tgcttaaccc cttaaacttg ttatttttta cttgaagcat tttgggatgg tcttaacagg
     6541 gaagagagag ggtgggggag aaaatgtttt tttctaagat tttccacaga tgctatagta
     6601 ctattgacaa actgggttag agaaggagtg taccgctgtg ctgttggcac gaacaccttc
     6661 agggactgga gctgctttta tccttggaag agtattccca gttgaagctg aaaagtacag
     6721 cacagtgcag ctttggttca tattcagtca tctcaggaga acttcagaag agcttgagta
     6781 ggccaaatgt tgaagttaag ttttccaata atgtgacttc ttaaaagttt tattaaaggg
     6841 gaggggcaaa tattggcaat tagttggcag tggcctgtta cggttgggat tggtggggtg
     6901 ggtttaggta attgtttagt ttatgattgc agataaactc atgccagaga acttaaagtc
     6961 ttagaatgga aaaagtaaag gaatatcaac ttccaagttg gcaagtaact cccaatgatt
     7021 tagttttttt ccccccagtt tgaattggga agctggggga agttaaatat gagccactgg
     7081 gtgtaccagt gcattaattt gggcaaggaa agtgtcataa tttgatactg tatctgtttt
     7141 ccttcaaagt atagagcttt tggggaagga aagtattgaa ctgggggttg gtctggccta
     7201 ctgggctgac attaactaca attatgggaa atgcaaaagt tgtttggata tggtagtgtg
     7261 tggttctctt ttggaatttt tttcaggtga tttaataata atttaaaact actatagaaa
     7321 ctgcagagca aaggaagtgg cttaatgatc ctgaagggat ttcttctgat ggtagctttt
     7381 gtattatcaa gtaagattct attttcagtt gtgtgtatgc aagttttttt ttagtgtagg
     7441 agaaataaac aagatgttaa ggtatgcttc aaaaattttg taaattgttt attttaaact
     7501 tatctgtttg taaattgtaa ctgattaaga attgtgatag ttcagcttga atgtctctta
     7561 gagggtgggc ttttgttgat gagggagggg aaactttttt tttttctata gacttttttc
     7621 agataacatc ttctgagtca taaccagcct ggcagtatga tggcctagat gcagagaaaa
     7681 cagctccttg gtgaattgat aagtaaaggc agaaaagatt atatgtcata cctccattgg
     7741 ggaataagca taaccctgag attcttacta ctgatgagaa cattatctgc atatgccaaa
     7801 aaattttaag caaatgaaag ctaccaattt aaagttacgg aatctaccat tttaaagtta
     7861 attgcttgtc aagctataac cacaaaaata atgaattgat gagaaataca atgaagaggc
     7921 aatgtccatc tcaaaatact gcttttacaa aagcagaata aaagcgaaaa gaaatgaaaa
     7981 tgttacacta cattaatcct ggaataaaag aagccgaaat aaatgagcca atgtttcgtt
     8041 tgcctcagac aggtatctct tcgttatcag aagagttgct tcatttcatc tgggagcaga
     8101 aaacagcagg cagctgttaa cagataagtt taacttgcat ctgcagtatt gcatgttagg
     8161 gataagtgct tatttttaag agctgtggag ttcttaaata tcaaccatgg cactttctcc
     8221 tgaccccttc cctaggggat ttcaggattg agaaattttt ccatcgagcc tttttaaaat
     8281 tgtaggactt gttcctgtgg gcttcagtga tgggatagta cacttcactc agaggcattt
     8341 gcatctttaa ataatttctt aaaagcctct aaagtgatca gtgccttgat gccaactaag
     8401 gaaatttgtt tagcattgaa tctctgaagg ctctatgaaa ggaatagcat gatgtgctgt
     8461 tagaatcaga tgttactgct aaaatttaca tgttgtgatg taaattgtgt agaaaaccat
     8521 taaatcattc aaaataataa actattttta ttagagaatg tatactttta gaaagctgtc
     8581 tccttattta aataaaatag tgtttgtctg tagttcagtg ttggggcaat cttggggggg
     8641 attcttctct aatctttcag aaactttgtc tgcgaacact ctttaatgga ccagatcagg
     8701 atttgagcgg aagaacgaat gtaactttaa ggcaggaaag acaaatttta ttcttcataa
     8761 agtgatgagc atataataat tccaggcaca tggcaataga ggccctctaa ataaggaata
     8821 aataacctct tagacaggtg ggagattatg atcagagtaa aaggtaatta cacattttat
     8881 ttccagaaag tcaggggtct ataaattgac agtgattaga gtaatacttt ttcacatttc
     8941 caaagtttgc atgttaactt taaatgctta caatcttaga gtggtaggca atgttttaca
     9001 ctattgacct tatataggga ggttttaaag aattttcctt tgcagaggca tttcatcctt
     9061 catgaagcca ttcaggattt tgaattgcat atgagtgctt ggctcttcct tctgttctag
     9121 tgagtgtatg agaccttgca gtgagtttat cagcatactc aaaatttttt tcctggaatt
     9181 tggagggatg ggaggagggg gtggggctta cttgttgtag cttttttttt ttacagactt
     9241 cacagagaat gcagttgtct tgacttcagg tttgtctgtt ctgttggcaa gtaaatgcag
     9301 tactgttctg atcccgctgc tattagaatg cattgtgaaa cgactggagt atgattaaaa
     9361 gttgtgttcc ccaatgcttg gagtagtgat tgttgaagga aaaaatccag ctgagtgata
     9421 aaggctgagt gttgaggaaa tttctgcagt tttaagcagt cgtatttgtg attgaagctg
     9481 agtacatttt gctggtgtat ttttaggtaa aatgcttttt gttcatttct ggtggtggga
     9541 ggggactgaa gcctttagtc ttttccagat gcaaccttaa aatcagtgac aagaaacatt
     9601 ccaaacaagc aacagtcttc aagaaattaa actggcaagt ggaaatgttt aaacagttca
     9661 gtgatcttta gtgcattgtt tatgtgtggg tttctctctc ccctcccttg gtcttaattc
     9721 ttacatgcag gaacactcag cagacacacg tatgcgaagg gccagagaag ccagacccag
     9781 taagaaaaaa tagcctattt actttaaata aaccaaacat tccattttaa atgtggggat
     9841 tgggaaccac tagttctttc agatggtatt cttcagacta tagaaggagg gacaaaatga
     9901 ggaaaacagg tgaacaagct ttttctgtat ttacatacaa agtcagatca gttatgggac
     9961 aatagtattg aatagatttc agctttatgc tggagtaact ggcatgtgag caaactgtgt
    10021 tggcgtgggg gtggaggggt gaggtgggcg ctaagccttt ttttaagatt tttcaggtac
    10081 ccctcactaa aggcaccgaa ggcttaaagt aggacaacca tggagccttc ctgtggcagg
    10141 agagacaaca aagcgctatt atcctaaggt caagagaagt gtcagcctca cctgcctcaa
    10201 ctccctcttt ctggagtgaa gcatccgaag gaatgcttga agtacccctg ggcttctctt
    10261 aacatttaag caagctgttt ttatagcagc tcttaataat aaagcccaaa tctcaagcgg
    10321 tgcttgaagg ggagggaaag ggggaaagcg ggcagccact tttccctagc ttttccagaa
    10381 gcctgttaaa agcaaggtct ccccacaagc aacttctctg ccacatcgcc accccgtgcc
    10441 ttttgatcta gcacagaccc ttcacccctc acctcgatgc agccagtagc ttggatcctt
    10501 gtgggcatga tccataatcg gtttcaaggt aacgatggtg tcgaggtctt tggtgggttg
    10561 aactatgtta gaaaaggcca ttaatttgcc tgcaaattgt taacagaagg gtattaaaac
    10621 cacagctaag tagctctatt ataatactta tccagtgact aaaaccaact taaaccagta
    10681 agtggagaaa taacatgttc aagaactgta atgctgggtg ggaacatgta acttgtagac
    10741 tggagaagat aggcatggga atgcaaaaat tctctgctaa gactttttca ggtgaacata
    10801 acagacttgg ccaagctagc atcttagcgg aagctgatct ccaatgctct tcagtagggt
    10861 catgaaggtt tttcttttcc tgagaaaaca acacgtattg ttttctcagg ttttgctttt
    10921 tggccttttt ctagcttaaa aaaaaaaaaa gcaaaagatg ctggtggttg gcactcctgg
    10981 tttccaggac ggggttcaga tccctgcggc gtctttgctt tgactactaa tctgtcttca
    11041 ggactctttc tgtatttctc cttttctctg caggtgctag ttcttggagt tttggggagg
    11101 tgggagataa cagcacaata tctttgaact atatacatcc ttgatgtata atttgtcagg
    11161 agcttgactt gattgtatat tcatatttac acgagaacct aatataactg ccttgtcttt
    11221 ttcaggtaat agcctgcagc tggtgttttg agaagcccta ctgctgaaaa cttaacaatt
    11281 ttgtgtaata aaaatggaga agctctaaat tgttgtggtt cttttgtgaa taaaaaaatc
    11341 ttgattgggg aaaaaaaaac tcgagggccg cgaaggatct gcgatcgctc cggtgcccgt
    11401 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
    11461 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
    11521 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
    11581 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
    11641 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
    11701 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
    11761 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
    11821 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
    11881 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
    11941 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
    12001 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
    12061 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
    12121 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
    12181 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
    12241 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
    12301 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
    12361 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
    12421 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
    12481 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
    12541 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
    12601 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
    12661 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
    12721 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
    12781 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    12841 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
    12901 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
    12961 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    13021 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    13081 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    13141 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    13201 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    13261 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    13321 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
    13381 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    13441 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    13501 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    13561 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    13621 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    13681 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    13741 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    13801 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    13861 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttgggcc
    13921 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    13981 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
    14041 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    14101 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    14161 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    14221 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
    14281 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
    14341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    14401 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
    14461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    14521 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    14581 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
    14641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    14701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    14761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    14821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    14881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    14941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    15001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    15061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    15121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    15181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    15241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    15301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    15361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    15421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    15481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    15541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    15601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    15661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    15721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    15781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    15841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    15901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    15961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    16021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    16081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    16141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    16201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    16261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    16321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    16381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    16441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    16501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    16561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    16621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    16681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    16741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    16801 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    16861 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    16921 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    16981 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    17041 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    17101 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    17161 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    17221 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
