LOCUS       sequence-311874-       14596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
  TITLE     Peroxisome compartmentalization of a toxic enzyme improves alkaloid 
            production.
  JOURNAL   Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4.
  PUBMED    33046851
REFERENCE   2  (bases 1 to 14596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4."
COMMENT     Sequence Label: pPSG1059
FEATURES             Location/Qualifiers
     source          1..14596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..680
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     550..573
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        1759..2172
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        10282..10689
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10690..11784
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    12266..12769
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(12813..13628)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12813..13628)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13536..13555
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13620..13639
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      13853..14441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14342..14361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
       61 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
      121 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
      181 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
      241 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
      301 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
      361 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
      421 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
      481 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
      541 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
      601 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
      661 aacacacata aacaaacaaa agatctatgg aagaaactgt aatgttatat cacgaattgg
      721 aagtcgcagc ctcagctgac gaagtatggt ctgtcgaagg ttctcctgaa ttaggtttac
      781 atttaccaga tttgttacca gctggaatat tcgcaaagtt tgagataact ggagatggtg
      841 gagaaggttc tattttggac atgacatttc cacctggtca attccctcat cattatagag
      901 agaagttcgt tttcttcgac cataagaata gatataagtt ggttgaacaa attgacggtg
      961 atttctttga tttgggagta acctactaca tggacactat tagggtcgtc gcaacaggtc
     1021 ctgattcttg tgtcatcaaa tcaacaactg aatatcatgt taaaccagaa tttgctaaga
     1081 ttgttaaacc tttaatcgat acagttccat tagccattat gtcagaggca atagctaagg
     1141 tagtattgga aaataagcac aaatcttctg agggatccgg ttcattggga agaggtagaa
     1201 gatccaaatt gtaataactc gagtggcagt gcttttaact aagaattatt agtcttttct
     1261 gcttattttt tcatcatagt ttagaacact ttatattaac gaatagttta tgaatctatt
     1321 taggtttaaa aattgataca gttttataag ttactttttc aaagactcgt gctgtctatt
     1381 gcataatgca ctggaagggg aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc
     1441 agtttgaaaa atgctgccaa accagatgtc aacacagcta caacgccttg ccaacaggga
     1501 gttcttcaga gacatggagg ctcaaaacga aattattgac agcctagaca tcaatagtca
     1561 tacaacagaa agcgaccacc caactttggc tgataatagc gtataaacaa tgcatacttt
     1621 gtacgttcaa aatacaatgc agtagatata tttatgcata ttacatataa tacatatcac
     1681 ataggaagca acaggcgcgt tggactttta attttcgagg accgcgaatc cttacatcac
     1741 acccaatccc ccacaagtga tcccccacac accatagctt caaaatgttt ctactccttt
     1801 tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa aacacccaag
     1861 cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta cccgtactaa
     1921 aggtttggaa aagaaaaaag acaccgcctc gtttcttttt cttcgtcgaa aaaggcaata
     1981 aaaattttta tcacgtttct ttttcttgaa aatttttttt tttgattttt ttctctttcg
     2041 atgacctccc attgatattt aagttaataa acggtcatca atttctcaag tttcagtttc
     2101 atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga aagcatagca
     2161 atctaatcta agttttaatt acaaaagatc tatggaagta gttactgtag cattgatagc
     2221 cgtcattata tcatctatat tgtatttgtt gttcggttct tctggacata agaatttacc
     2281 acctggtcct aagccatggc ctatagtagg taacttatta cagttgggtg agaagccaca
     2341 cgcacaattc gctgagttag ctcagactta tggtgatatc ttcactttaa agatgggtac
     2401 agagacagtc gtagttgctt caacttcttc agcagcatca gaaattttaa agacccatga
     2461 cagaatcttg tctgctaggt acgttttcca gtcttttaga gtaaaaggtc atgttgaaaa
     2521 ttctatagtc tggtctgact gcacagaaac ttggaagaac ttaagaaaag tatgtagaac
     2581 tgaattgttt acacaaaaga tgatagaatc tcaagctcat gtcagagaaa agaagtgtga
     2641 agagatggtt gagtatttga tgaagaaaca aggagaagaa gttaaaattg tagaagttat
     2701 ctttggaact ttggtcaata tattcggtaa cttgattttc tcacaaaaca tattcgaatt
     2761 gggtgatcct aattctggtt cttcagagtt taaggaatat ttatggagaa tgttagaatt
     2821 aggtaattct actaatcctg ctgattattt tccaatgttg ggtaagtttg atttgtttgg
     2881 tcaaaggaaa gaagttgctg agtgcttaaa aggaatttat gcaatatggg gtgctatgtt
     2941 acaagagaga aaattggcta agaaagttga tggatatcaa tctaagaacg attttgtaga
     3001 tgtatgttta gattcaggtt tgaacgatta tcagattaat gcattattga tggaattatt
     3061 cggagctggt actgaaacct cagcctcaac aatagaatgg gctatgacag aattaaccaa
     3121 gaatccaaag attacagcca agttaaggtc agagttacaa accgtcgttg gagaaagatc
     3181 agttaaggaa tctgattttc caaatttacc ttatttagaa gctaccgtta aggaaacttt
     3241 aaggttgcat ccacctacac ctttgttgtt gccaaggaga gctttggaaa cttgcactat
     3301 attaaattac accattccaa aagactgtca aattatggta aacgcttggg gaatcggtag
     3361 ggaccctaag acatggatag atcctttgac tttctcacca gagagattct tgaactcttc
     3421 tgtcgacttc aggggaaatg atttctcatt aatccctttt ggtgctggta ggagaatttg
     3481 tcctggttta cctatcgcta atcaatttat tgcattatta gtcgcaacct ttgttcaaaa
     3541 cttagattgg tgtttgccaa atggtatgtc agttgatcat ttgatagtag aggaaaagtt
     3601 cggtttaaca ttgcaaaagg aaccaccatt atttatcgtc ccaaaatcaa gggttggatc
     3661 ctaactcgag ataaagcaat cttgatgagg ataatgattt ttttttgaat atacataaat
     3721 actaccgttt ttctgctaga ttttgtgatg acgtaaataa gtacatatta ctttttaagc
     3781 caagacaaga ttaagcatta actttaccct tttctttcta agtttcaata ttagttatca
     3841 ctgtttaaaa gttatggcga gaacgtcggc ggttaaaata tattaccctg aacggctgga
     3901 tgcacacact ggcttaagat gacaacgcac ccatgaacca cacggttagt ccaaaagggg
     3961 cagttcagat tccagatgcg ggaattagct tgctgccacc ctcacctcac taacgctgcg
     4021 gtgtgcggat acttcatgct atttatagac gcgcgtgtcg gaatcagcac gcgcaagaac
     4081 caaatgggaa aatcggaatg ggtccagaac tgctttgagt gctggctatt ggcgtctgat
     4141 ttccgttttg ggaatccttt gccgcgcgcc cctctcaaaa ctccgcacaa gtcccagaaa
     4201 gcgggaaaga aataaaacgc caccaaaaaa aaaaaaataa aagccaatcc tcgaagcgtg
     4261 ggtggtaggc cctggattat cccgtacaag tatttctcag gagtaaaaaa accgtttgtt
     4321 ttggaatttc ccatttcgcg gccacctacg ccgctatctt tgcaacaact atctgcgata
     4381 actcagcaaa ttttgcatat tcgtgttgca gtattgcgat aatgggagtc ttacttccaa
     4441 cataacggca gaaagaaatg tgagaaaatt ttgcatcctt tgcctccgtt caagtatata
     4501 aagtcggcat gcttgataat ctttctttcc atcctacatt gttctaatta ttcttattct
     4561 cctttattct ttcctaacat accaagaaat taatcttctg tcattcgctt aaacactata
     4621 tcaataaaga tctatggaaa cagtttctaa gatagatcag caaaaccagg ctaaaatttg
     4681 gaagcaaata tacggttttg ctgaatcttt agtcttgaaa tgcgccgttc agttggaaat
     4741 agccgaaact ttacataaca acgttaaacc aatgtcattg tctgaattag cttctaaatt
     4801 gccagtcgca caacctgtca acgaagatag attatttagg attatgaggt acttggtaca
     4861 tatggagttg tttaaaatcg atgctactac tcagaaatat tcattggccc ctcctgctaa
     4921 atatttatta aggggatggg aaaagtcaat ggttgattct attttgtgta tcaatgataa
     4981 agacttcttg gccccttggc atcatttggg agatggattg accggaaact gtgatgcttt
     5041 tgagaaggct ttaggtaaat ctatttgggt ttatatgtct gtaaatccag aaaagaatca
     5101 attattcaac gcagcaatgg cttgtgatac aaggttagtt acctctgctt tagctaacga
     5161 atgtaagtct atcttttcag atggtatatc aactttagtt gacgtaggtg gaggtactgg
     5221 aactgccgtc aaagctatat ctaaagcttt tccagatatt aaatgtacca tatacgattt
     5281 accacacgtt atcgccgact ctccagagat tccaaatatt actaagattt ctggtgatat
     5341 gttcaaatct atcccatctg ccgacgcaat tttcatgaaa tgcattttgc acgattggaa
     5401 cgatgatgaa tgcatacaaa ttttgaaaag atgtaaagaa gcattaccta aaggaggtaa
     5461 agtaatcatt gtagatgtcg taattgatat ggactctaca cacccatatg caaagattag
     5521 attgacctta gacttagaca tgatgttgaa taccggtggt aaagaaagaa caaaagaaga
     5581 atggaagaca ttatttgacg ctgctggttt tgcctcacat aaggttactc aaatctctgc
     5641 tgtccaatca gtaatagagg cctacccata tggatcctaa ctcgagattg aattgaattg
     5701 aaatcgatag atcaattttt ttcttttctc tttccccatc ctttacgcta aaataatagt
     5761 ttattttatt ttttgaatat tttttattta tatacgtata tatagactat tatttatctt
     5821 ttaatgatta ttaagatttt tattaaaaaa aaattcgctc ctcttttaat gcctttatgc
     5881 agtttttttt tcccattcga tatttctatg tgctggttct gtatgggcac agacaaccta
     5941 aacggtgagt aaggaaagag tgaggaacta tcgcatacct gcatttaaag atgccgattt
     6001 gggcgcgaat cctttatttt ggcttcaccc tcatactatt atcagggcca gaaaaaggaa
     6061 gtgtttccct ccttcttgaa ttgatgttac cctcataaag cacgtggcct cttatcgaga
     6121 aagaaattac cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa aaacccagac
     6181 acgctcgact tcctgtcatc ctattgattg cagcttccaa tttcgtcaca caacaaggtc
     6241 ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg gcgggaaagg
     6301 gtttagtacc acatgctatg atgcccactg tgatctccag agcaaagttc gttcgatcgt
     6361 actgttactc tctctctttc aaacagaatt gtccgaatcg tgtgacaaca acagcctgtt
     6421 ctcacacact cttttcttct aaccaagggg gtggtttagt ttagtagaac ctcgtgaaac
     6481 ttacatttac atatatataa acttgcataa attggtcaat gcaagaaata catatttggt
     6541 cttttctaat tcgtagtttt tcaagttctt agatgctttc tttttctctt ttttacagat
     6601 catcaaggaa gtaattatct actttttaca acaaatataa aacaagatct atgggatcat
     6661 tggacgctaa accagctgct gcaactcagg aagtctctat taaggatcaa gcacagttat
     6721 ggaatatcat ttacggtttc gccgattctt tagttttaag atgtgcagtt gagatcggta
     6781 tagcagatat tattaagaat aacgatggtg ccattacctt agcccaattg gccgcaaaat
     6841 tacctattac taatgtctct tcagattatt tatacagaat ggttagatac ttggtacatt
     6901 taaatattat agaacaagag acatgtaatg gtggagttga gaaagtatac tctttgaagc
     6961 cagttggaac attattgttg agagatgcag aaaggtcaat ggtcccaatg atcttgggta
     7021 tgactcagaa agatttcatg gtatcttggc actttatgaa agagggattg ggaaatggat
     7081 ctacaactgc ctttgaaaag ggtatgggta tggacatctg gaaatatttg gaaggtaatc
     7141 cagatcagtc acaattgttt aatgaaggta tggctggtga gactagattg ttaacaaaga
     7201 ctttaataga ggattgcaga gatacatttc aaggtttaga ctctttagtt gatattggtg
     7261 gtggaaacgg aacaaccata aaggctatct atgaagcatt ccctcatatt aaatgtacct
     7321 tatacgattt gccacatgta gtcgctaatt cacatgactt gccaaacatt gagaaagtac
     7381 ctggtgacat gtttaagtct gttccatctg ctcaagctat attattaaag ttgatcttgc
     7441 acgattggac tgacgaggaa tgtgtaaaca tcttgaagaa atgcaaagaa gccattccta
     7501 aagaaacagg aaaggttatt atagttgatg tcgctttgga ggaagaatct aaccatgaat
     7561 tgactaaaac tagattaatt ttggacatcg atatgttggt taatacaggt ggaagggaga
     7621 ggactgctga tgattgggaa aacttattga agagagcagg attcagatca cataagatca
     7681 gacctatcag agcaatacag tctgtcatcg aagcctttcc aggatcctaa ctcgagagct
     7741 tttgattaag ccttctagtc caaaaaacac gtttttttgt catttatttc attttcttag
     7801 aatagtttag tttattcatt ttatagtcac gaatgtttta tgattctata tagggttgca
     7861 aacaagcatt tttcatttta tgttaaaaca atttcaggtt taccttttat tctgcttgtg
     7921 gtgacgcgtg tatccgcccg ctcttttggt cacccatgta tgctgggtag actacccatg
     7981 agtcacaatg aacgttgata ggtcaagatc aatgtaaaca attactttgt tatgtagagt
     8041 ttttttagct acctatattc caccataaca tcaatcatgc ggttgctggt gtatttacca
     8101 ataatgttta atgtatatat atatatatat atatggggcc gtatacttac atatagtaga
     8161 tgtcaagcgt aggcgcttcc cctgccggct gtgagggcgc cataaccaag gtatctatag
     8221 accgccaatc agcaaactac ctccgtacat tcatgttgca cccacacatt tatacaccca
     8281 gaccgcgaca aattacccat aaggttgttt gtgacggcgt cgtacaagag aacgtgggaa
     8341 ctttttaggc tcaccaaaaa agaaagaaaa aatacgagtt gctgacagaa gcctcaagaa
     8401 aaaaaaaatt cttcttcgac tatgctggag gcagagatga tcgagccggt agttaactat
     8461 atatagctaa attggttcca tcaccttctt ttctggtgtc gctccttcta gtgctatttc
     8521 tggcttttcc tatttttttt tttccatttt tctttctctc tttctaatat ataaattctc
     8581 ttgcattttc tatttttctc tctatctatt ctacttgttt attcccttca aggttttttt
     8641 ttaaggagta cttgttttta gaatatacgg tcaacgaact ataattaact aaacagatct
     8701 atgcagttaa aagctaaaga ggagttgtta agaaatatgg agttaggttt gattcctgat
     8761 caagaaataa gacaattgat cagagttgaa ttagagaaga gattgcaatg gggttataaa
     8821 gaaacccacg aagaacaatt gtcacaattg ttggatttgg ttcactcttt aaaaggtatg
     8881 aaaatggcca ccgagatgga gaatttggac ttgaaattat acgaggctcc tatggagttc
     8941 ttgaagatcc aacacggttc aaatatgaag caatctgctg gttactatac agatgaatct
     9001 acaactttgg atgaggccga aattgcaatg ttggacttgt atatggagag agcccaaatt
     9061 aaagatggac aatcagtctt agatttaggt tgcggattgg gagccgttgc cttatttgga
     9121 gcaaataagt ttaagaaatg tcagttcacc ggtgttacct cttcagtaga acaaaaggac
     9181 tacatcgaag gtaaatgcaa agaattaaag ttaactaatg taaaagtttt attagcagat
     9241 ataaccacct atgagactga agaaagattt gataggatct tcgctgtcga attgatagag
     9301 catatgaaga attaccaatt attattgaag aaaatttctg aatggatgaa agacgacggt
     9361 ttgttattcg tagaacacgt ctgtcataaa actttggctt accattatga acctgttgat
     9421 gctgaggatt ggtacacaaa ctacatcttt ccagctggta ctttgacctt gtcatcagca
     9481 tctatgttgt tgtattttca agatgatgtc tctgtagtta atcagtggac attgtctggt
     9541 aagcactatt caagatcaca tgaagaatgg ttgaagaata tggataagaa cattgtagag
     9601 ttcaaagaga taatgaggtc tatcaccaag acagagaaag aggctataaa attgttaaat
     9661 ttctggagga tcttctgcat gtgtggtgct gagttattcg gttacaagaa cggtgaagaa
     9721 tggatgttga cacatttatt atttaagaag aaaggatcct aactcgaggc caattggtgc
     9781 ggcaattgat aataacgaaa atgtctttta atgatctggg tataatgagg aattttccga
     9841 acgtttttac tttatatata tatatacatg taacatatat tctatacgct atagagaaag
     9901 gaaatttttc aattaaaaaa aaaatagaga aagagtttca cttcttgatt atcgctaaca
     9961 ctaatggttg aagtactgct actttaattt tatgctgagc agttacagag atgttacgaa
    10021 ccactagtgc actgcagtac atcgaggaga acttctagta tatctacata cctaatatta
    10081 ttgccttatt aaaaatggaa tcccaacaat tacatcaaaa tccacattct cttcaaaatc
    10141 aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg ttatatttat aggataatta
    10201 tactctattt ctcaacaagt aattggttgt ttggccgagc ggtctaaggc gcctgattca
    10261 agaaatatct tgaccgcagt taactgtggg aatactcagg tatcgtaaga tgcaagagtt
    10321 cgaatctctt agcaaccatt atttttttcc tcaacataac gagaacacac aggggcgcta
    10381 tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg
    10441 catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc gccggaaccg
    10501 gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag
    10561 ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac
    10621 tttctaactt ttcttacctt ttacatttca gcaatatata tatatatatt tcaaggatat
    10681 accattctaa tgtctgcccc taagaagatc gtcgttttgc caggtgacca cgttggtcaa
    10741 gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg ttccaatgtc
    10801 aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac aggtgttcca
    10861 cttccagatg aggcgctgga agcctccaag aaggctgatg ccgttttgtt aggtgctgtg
    10921 ggtggtccta aatggggaac cggtagtgtt agacctgaac aaggtttact aaaaatccgt
    10981 aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga ctctctttta
    11041 gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt tgtcagagaa
    11101 ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg
    11161 gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc cgctttcatg
    11221 gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa tgttttggcc
    11281 tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt tcctacattg
    11341 aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa cccaacccac
    11401 ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga tgaagcctcc
    11461 gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt gccagacaag
    11521 aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt gccaaagaat
    11581 aaggtcaacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt gtcattgaac
    11641 ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga tgcaggtatc
    11701 agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa
    11761 gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacataaac
    11821 tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat atgttcatag
    11881 ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa aaaggaggat
    11941 gtaaaggaat acaggtaagc aaattgatac taatggctca acgtgataag gaaaaagaat
    12001 tgcactttaa cattaatatt gacaaggagg agggcaccac acaaaaagtt aggtgtaaca
    12061 gaaaatcatg aaactatgat tcctaattta tatattggag gattttctct aaaaaaaaaa
    12121 aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac
    12181 cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa
    12241 cggccttaac gacgtagtcg agagtatcac gtgctataaa aataattata atttaaattt
    12301 tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag
    12361 tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta
    12421 tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaaa
    12481 accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta
    12541 tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt
    12601 tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac
    12661 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
    12721 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcccgagcg
    12781 gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
    12841 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
    12901 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
    12961 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
    13021 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
    13081 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
    13141 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg
    13201 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
    13261 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
    13321 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
    13381 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
    13441 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
    13501 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
    13561 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
    13621 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
    13681 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
    13741 tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg
    13801 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13861 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13921 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13981 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    14041 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    14101 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    14161 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    14221 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    14281 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    14341 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    14401 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    14461 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    14521 ctgattctgt ggataaccgt gcggccgccc ctgaattcgc atctagactg aactggccga
    14581 taattgcaga cgaacg
//
