LOCUS       sequence-311875-       14596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
  TITLE     Peroxisome compartmentalization of a toxic enzyme improves alkaloid 
            production.
  JOURNAL   Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4.
  PUBMED    33046851
REFERENCE   2  (bases 1 to 14596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4."
COMMENT     Sequence Label: pPSG1060
FEATURES             Location/Qualifiers
     source          1..14596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        1746..2159
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        10269..10676
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10677..11771
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    12253..12756
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(12800..13615)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12800..13615)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13523..13542
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13607..13626
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      13840..14428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14329..14348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctatggaag aaactgtaat gttatatcac gaattggaag tcgcagcctc
      721 agctgacgaa gtatggtctg tcgaaggttc tcctgaatta ggtttacatt taccagattt
      781 gttaccagct ggaatattcg caaagtttga gataactgga gatggtggag aaggttctat
      841 tttggacatg acatttccac ctggtcaatt ccctcatcat tatagagaga agttcgtttt
      901 cttcgaccat aagaatagat ataagttggt tgaacaaatt gacggtgatt tctttgattt
      961 gggagtaacc tactacatgg acactattag ggtcgtcgca acaggtcctg attcttgtgt
     1021 catcaaatca acaactgaat atcatgttaa accagaattt gctaagattg ttaaaccttt
     1081 aatcgataca gttccattag ccattatgtc agaggcaata gctaaggtag tattggaaaa
     1141 taagcacaaa tcttctgagg gatccggttc attgggaaga ggtagaagat ccaaaaccta
     1201 ataactcgag tggcagtgct tttaactaag aattattagt cttttctgct tattttttca
     1261 tcatagttta gaacacttta tattaacgaa tagtttatga atctatttag gtttaaaaat
     1321 tgatacagtt ttataagtta ctttttcaaa gactcgtgct gtctattgca taatgcactg
     1381 gaaggggaaa aaaaaggtgc acacgcgtgg ctttttcttg aatttgcagt ttgaaaaatg
     1441 ctgccaaacc agatgtcaac acagctacaa cgccttgcca acagggagtt cttcagagac
     1501 atggaggctc aaaacgaaat tattgacagc ctagacatca atagtcatac aacagaaagc
     1561 gaccacccaa ctttggctga taatagcgta taaacaatgc atactttgta cgttcaaaat
     1621 acaatgcagt agatatattt atgcatatta catataatac atatcacata ggaagcaaca
     1681 ggcgcgttgg acttttaatt ttcgaggacc gcgaatcctt acatcacacc caatccccca
     1741 caagtgatcc cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag
     1801 attttctcgg actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa
     1861 atttcccctc tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag
     1921 aaaaaagaca ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca
     1981 cgtttctttt tcttgaaaat tttttttttt gatttttttc tctttcgatg acctcccatt
     2041 gatatttaag ttaataaacg gtcatcaatt tctcaagttt cagtttcatt tttcttgttc
     2101 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt
     2161 tttaattaca aaagatctat ggaagtagtt actgtagcat tgatagccgt cattatatca
     2221 tctatattgt atttgttgtt cggttcttct ggacataaga atttaccacc tggtcctaag
     2281 ccatggccta tagtaggtaa cttattacag ttgggtgaga agccacacgc acaattcgct
     2341 gagttagctc agacttatgg tgatatcttc actttaaaga tgggtacaga gacagtcgta
     2401 gttgcttcaa cttcttcagc agcatcagaa attttaaaga cccatgacag aatcttgtct
     2461 gctaggtacg ttttccagtc ttttagagta aaaggtcatg ttgaaaattc tatagtctgg
     2521 tctgactgca cagaaacttg gaagaactta agaaaagtat gtagaactga attgtttaca
     2581 caaaagatga tagaatctca agctcatgtc agagaaaaga agtgtgaaga gatggttgag
     2641 tatttgatga agaaacaagg agaagaagtt aaaattgtag aagttatctt tggaactttg
     2701 gtcaatatat tcggtaactt gattttctca caaaacatat tcgaattggg tgatcctaat
     2761 tctggttctt cagagtttaa ggaatattta tggagaatgt tagaattagg taattctact
     2821 aatcctgctg attattttcc aatgttgggt aagtttgatt tgtttggtca aaggaaagaa
     2881 gttgctgagt gcttaaaagg aatttatgca atatggggtg ctatgttaca agagagaaaa
     2941 ttggctaaga aagttgatgg atatcaatct aagaacgatt ttgtagatgt atgtttagat
     3001 tcaggtttga acgattatca gattaatgca ttattgatgg aattattcgg agctggtact
     3061 gaaacctcag cctcaacaat agaatgggct atgacagaat taaccaagaa tccaaagatt
     3121 acagccaagt taaggtcaga gttacaaacc gtcgttggag aaagatcagt taaggaatct
     3181 gattttccaa atttacctta tttagaagct accgttaagg aaactttaag gttgcatcca
     3241 cctacacctt tgttgttgcc aaggagagct ttggaaactt gcactatatt aaattacacc
     3301 attccaaaag actgtcaaat tatggtaaac gcttggggaa tcggtaggga ccctaagaca
     3361 tggatagatc ctttgacttt ctcaccagag agattcttga actcttctgt cgacttcagg
     3421 ggaaatgatt tctcattaat cccttttggt gctggtagga gaatttgtcc tggtttacct
     3481 atcgctaatc aatttattgc attattagtc gcaacctttg ttcaaaactt agattggtgt
     3541 ttgccaaatg gtatgtcagt tgatcatttg atagtagagg aaaagttcgg tttaacattg
     3601 caaaaggaac caccattatt tatcgtccca aaatcaaggg ttggatccta actcgagata
     3661 aagcaatctt gatgaggata atgatttttt tttgaatata cataaatact accgtttttc
     3721 tgctagattt tgtgatgacg taaataagta catattactt tttaagccaa gacaagatta
     3781 agcattaact ttaccctttt ctttctaagt ttcaatatta gttatcactg tttaaaagtt
     3841 atggcgagaa cgtcggcggt taaaatatat taccctgaac ggctggatgc acacactggc
     3901 ttaagatgac aacgcaccca tgaaccacac ggttagtcca aaaggggcag ttcagattcc
     3961 agatgcggga attagcttgc tgccaccctc acctcactaa cgctgcggtg tgcggatact
     4021 tcatgctatt tatagacgcg cgtgtcggaa tcagcacgcg caagaaccaa atgggaaaat
     4081 cggaatgggt ccagaactgc tttgagtgct ggctattggc gtctgatttc cgttttggga
     4141 atcctttgcc gcgcgcccct ctcaaaactc cgcacaagtc ccagaaagcg ggaaagaaat
     4201 aaaacgccac caaaaaaaaa aaaataaaag ccaatcctcg aagcgtgggt ggtaggccct
     4261 ggattatccc gtacaagtat ttctcaggag taaaaaaacc gtttgttttg gaatttccca
     4321 tttcgcggcc acctacgccg ctatctttgc aacaactatc tgcgataact cagcaaattt
     4381 tgcatattcg tgttgcagta ttgcgataat gggagtctta cttccaacat aacggcagaa
     4441 agaaatgtga gaaaattttg catcctttgc ctccgttcaa gtatataaag tcggcatgct
     4501 tgataatctt tctttccatc ctacattgtt ctaattattc ttattctcct ttattctttc
     4561 ctaacatacc aagaaattaa tcttctgtca ttcgcttaaa cactatatca ataaagatct
     4621 atggaaacag tttctaagat agatcagcaa aaccaggcta aaatttggaa gcaaatatac
     4681 ggttttgctg aatctttagt cttgaaatgc gccgttcagt tggaaatagc cgaaacttta
     4741 cataacaacg ttaaaccaat gtcattgtct gaattagctt ctaaattgcc agtcgcacaa
     4801 cctgtcaacg aagatagatt atttaggatt atgaggtact tggtacatat ggagttgttt
     4861 aaaatcgatg ctactactca gaaatattca ttggcccctc ctgctaaata tttattaagg
     4921 ggatgggaaa agtcaatggt tgattctatt ttgtgtatca atgataaaga cttcttggcc
     4981 ccttggcatc atttgggaga tggattgacc ggaaactgtg atgcttttga gaaggcttta
     5041 ggtaaatcta tttgggttta tatgtctgta aatccagaaa agaatcaatt attcaacgca
     5101 gcaatggctt gtgatacaag gttagttacc tctgctttag ctaacgaatg taagtctatc
     5161 ttttcagatg gtatatcaac tttagttgac gtaggtggag gtactggaac tgccgtcaaa
     5221 gctatatcta aagcttttcc agatattaaa tgtaccatat acgatttacc acacgttatc
     5281 gccgactctc cagagattcc aaatattact aagatttctg gtgatatgtt caaatctatc
     5341 ccatctgccg acgcaatttt catgaaatgc attttgcacg attggaacga tgatgaatgc
     5401 atacaaattt tgaaaagatg taaagaagca ttacctaaag gaggtaaagt aatcattgta
     5461 gatgtcgtaa ttgatatgga ctctacacac ccatatgcaa agattagatt gaccttagac
     5521 ttagacatga tgttgaatac cggtggtaaa gaaagaacaa aagaagaatg gaagacatta
     5581 tttgacgctg ctggttttgc ctcacataag gttactcaaa tctctgctgt ccaatcagta
     5641 atagaggcct acccatatgg atcctaactc gagattgaat tgaattgaaa tcgatagatc
     5701 aatttttttc ttttctcttt ccccatcctt tacgctaaaa taatagttta ttttattttt
     5761 tgaatatttt ttatttatat acgtatatat agactattat ttatctttta atgattatta
     5821 agatttttat taaaaaaaaa ttcgctcctc ttttaatgcc tttatgcagt ttttttttcc
     5881 cattcgatat ttctatgtgc tggttctgta tgggcacaga caacctaaac ggtgagtaag
     5941 gaaagagtga ggaactatcg catacctgca tttaaagatg ccgatttggg cgcgaatcct
     6001 ttattttggc ttcaccctca tactattatc agggccagaa aaaggaagtg tttccctcct
     6061 tcttgaattg atgttaccct cataaagcac gtggcctctt atcgagaaag aaattaccgt
     6121 cgctcgtgat ttgtttgcaa aaagaacaaa actgaaaaaa cccagacacg ctcgacttcc
     6181 tgtcatccta ttgattgcag cttccaattt cgtcacacaa caaggtccta gcgacggctc
     6241 acaggttttg taacaagcaa tcgaaggttc tggaatggcg ggaaagggtt tagtaccaca
     6301 tgctatgatg cccactgtga tctccagagc aaagttcgtt cgatcgtact gttactctct
     6361 ctctttcaaa cagaattgtc cgaatcgtgt gacaacaaca gcctgttctc acacactctt
     6421 ttcttctaac caagggggtg gtttagttta gtagaacctc gtgaaactta catttacata
     6481 tatataaact tgcataaatt ggtcaatgca agaaatacat atttggtctt ttctaattcg
     6541 tagtttttca agttcttaga tgctttcttt ttctcttttt tacagatcat caaggaagta
     6601 attatctact ttttacaaca aatataaaac aagatctatg ggatcattgg acgctaaacc
     6661 agctgctgca actcaggaag tctctattaa ggatcaagca cagttatgga atatcattta
     6721 cggtttcgcc gattctttag ttttaagatg tgcagttgag atcggtatag cagatattat
     6781 taagaataac gatggtgcca ttaccttagc ccaattggcc gcaaaattac ctattactaa
     6841 tgtctcttca gattatttat acagaatggt tagatacttg gtacatttaa atattataga
     6901 acaagagaca tgtaatggtg gagttgagaa agtatactct ttgaagccag ttggaacatt
     6961 attgttgaga gatgcagaaa ggtcaatggt cccaatgatc ttgggtatga ctcagaaaga
     7021 tttcatggta tcttggcact ttatgaaaga gggattggga aatggatcta caactgcctt
     7081 tgaaaagggt atgggtatgg acatctggaa atatttggaa ggtaatccag atcagtcaca
     7141 attgtttaat gaaggtatgg ctggtgagac tagattgtta acaaagactt taatagagga
     7201 ttgcagagat acatttcaag gtttagactc tttagttgat attggtggtg gaaacggaac
     7261 aaccataaag gctatctatg aagcattccc tcatattaaa tgtaccttat acgatttgcc
     7321 acatgtagtc gctaattcac atgacttgcc aaacattgag aaagtacctg gtgacatgtt
     7381 taagtctgtt ccatctgctc aagctatatt attaaagttg atcttgcacg attggactga
     7441 cgaggaatgt gtaaacatct tgaagaaatg caaagaagcc attcctaaag aaacaggaaa
     7501 ggttattata gttgatgtcg ctttggagga agaatctaac catgaattga ctaaaactag
     7561 attaattttg gacatcgata tgttggttaa tacaggtgga agggagagga ctgctgatga
     7621 ttgggaaaac ttattgaaga gagcaggatt cagatcacat aagatcagac ctatcagagc
     7681 aatacagtct gtcatcgaag cctttccagg atcctaactc gagagctttt gattaagcct
     7741 tctagtccaa aaaacacgtt tttttgtcat ttatttcatt ttcttagaat agtttagttt
     7801 attcatttta tagtcacgaa tgttttatga ttctatatag ggttgcaaac aagcattttt
     7861 cattttatgt taaaacaatt tcaggtttac cttttattct gcttgtggtg acgcgtgtat
     7921 ccgcccgctc ttttggtcac ccatgtatgc tgggtagact acccatgagt cacaatgaac
     7981 gttgataggt caagatcaat gtaaacaatt actttgttat gtagagtttt tttagctacc
     8041 tatattccac cataacatca atcatgcggt tgctggtgta tttaccaata atgtttaatg
     8101 tatatatata tatatatata tggggccgta tacttacata tagtagatgt caagcgtagg
     8161 cgcttcccct gccggctgtg agggcgccat aaccaaggta tctatagacc gccaatcagc
     8221 aaactacctc cgtacattca tgttgcaccc acacatttat acacccagac cgcgacaaat
     8281 tacccataag gttgtttgtg acggcgtcgt acaagagaac gtgggaactt tttaggctca
     8341 ccaaaaaaga aagaaaaaat acgagttgct gacagaagcc tcaagaaaaa aaaaattctt
     8401 cttcgactat gctggaggca gagatgatcg agccggtagt taactatata tagctaaatt
     8461 ggttccatca ccttcttttc tggtgtcgct ccttctagtg ctatttctgg cttttcctat
     8521 tttttttttt ccatttttct ttctctcttt ctaatatata aattctcttg cattttctat
     8581 ttttctctct atctattcta cttgtttatt cccttcaagg ttttttttta aggagtactt
     8641 gtttttagaa tatacggtca acgaactata attaactaaa cagatctatg cagttaaaag
     8701 ctaaagagga gttgttaaga aatatggagt taggtttgat tcctgatcaa gaaataagac
     8761 aattgatcag agttgaatta gagaagagat tgcaatgggg ttataaagaa acccacgaag
     8821 aacaattgtc acaattgttg gatttggttc actctttaaa aggtatgaaa atggccaccg
     8881 agatggagaa tttggacttg aaattatacg aggctcctat ggagttcttg aagatccaac
     8941 acggttcaaa tatgaagcaa tctgctggtt actatacaga tgaatctaca actttggatg
     9001 aggccgaaat tgcaatgttg gacttgtata tggagagagc ccaaattaaa gatggacaat
     9061 cagtcttaga tttaggttgc ggattgggag ccgttgcctt atttggagca aataagttta
     9121 agaaatgtca gttcaccggt gttacctctt cagtagaaca aaaggactac atcgaaggta
     9181 aatgcaaaga attaaagtta actaatgtaa aagttttatt agcagatata accacctatg
     9241 agactgaaga aagatttgat aggatcttcg ctgtcgaatt gatagagcat atgaagaatt
     9301 accaattatt attgaagaaa atttctgaat ggatgaaaga cgacggtttg ttattcgtag
     9361 aacacgtctg tcataaaact ttggcttacc attatgaacc tgttgatgct gaggattggt
     9421 acacaaacta catctttcca gctggtactt tgaccttgtc atcagcatct atgttgttgt
     9481 attttcaaga tgatgtctct gtagttaatc agtggacatt gtctggtaag cactattcaa
     9541 gatcacatga agaatggttg aagaatatgg ataagaacat tgtagagttc aaagagataa
     9601 tgaggtctat caccaagaca gagaaagagg ctataaaatt gttaaatttc tggaggatct
     9661 tctgcatgtg tggtgctgag ttattcggtt acaagaacgg tgaagaatgg atgttgacac
     9721 atttattatt taagaagaaa ggatcctaac tcgaggccaa ttggtgcggc aattgataat
     9781 aacgaaaatg tcttttaatg atctgggtat aatgaggaat tttccgaacg tttttacttt
     9841 atatatatat atacatgtaa catatattct atacgctata gagaaaggaa atttttcaat
     9901 taaaaaaaaa atagagaaag agtttcactt cttgattatc gctaacacta atggttgaag
     9961 tactgctact ttaattttat gctgagcagt tacagagatg ttacgaacca ctagtgcact
    10021 gcagtacatc gaggagaact tctagtatat ctacatacct aatattattg ccttattaaa
    10081 aatggaatcc caacaattac atcaaaatcc acattctctt caaaatcaat tgtcctgtac
    10141 ttccttgttc atgtgtgttc aaaaacgtta tatttatagg ataattatac tctatttctc
    10201 aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga
    10261 ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
    10321 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
    10381 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
    10441 caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag
    10501 aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat
    10561 ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc
    10621 ttacctttta catttcagca atatatatat atatatttca aggatatacc attctaatgt
    10681 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
    10741 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
    10801 aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg
    10861 cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat
    10921 ggggaaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
    10981 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
    11041 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
    11101 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
    11161 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
    11221 agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat
    11281 ggagaaaaac tgtggaggaa accatcaaga acgaatttcc tacattgaag gttcaacatc
    11341 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
    11401 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
    11461 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
    11521 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta
    11581 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
    11641 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
    11701 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
    11761 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
    11821 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
    11881 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
    11941 ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat
    12001 taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa
    12061 ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat
    12121 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat
    12181 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac
    12241 gtagtcgaga gtatcacgtg ctataaaaat aattataatt taaatttttt aatataaata
    12301 tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg
    12361 aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct tgctcttcct
    12421 gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaaaacc acacacgaaa
    12481 atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt aatctgcttt
    12541 tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt aaacctttgt
    12601 ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca
    12661 aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa
    12721 agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc ccgagcggcc gcgtgttaca
    12781 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
    12841 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
    12901 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
    12961 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
    13021 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
    13081 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
    13141 cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac
    13201 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
    13261 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
    13321 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
    13381 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
    13441 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
    13501 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
    13561 tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac
    13621 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
    13681 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
    13741 cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt tttcgttcca
    13801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    13861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    13921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    13981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    14041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    14101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    14161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    14221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    14281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    14341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    14401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    14461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    14521 taaccgtgcg gccgcccctg aattcgcatc tagactgaac tggccgataa ttgcagacga
    14581 acgcagttcg agttta
//
