LOCUS       sequence-311876-        7538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tNCS-Venus (CEN6/ARS4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
  TITLE     Peroxisome compartmentalization of a toxic enzyme improves alkaloid 
            production.
  JOURNAL   Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4.
  PUBMED    33046851
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4."
COMMENT     Sequence Label: pPSG1067
FEATURES             Location/Qualifiers
     source          1..7538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             1172..1882
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     promoter        2589..2996
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2997..4091
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      4573..5555
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5613..6428)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5613..6428)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6336..6355
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6420..6439
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6653..7241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7142..7161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctatggaag aaactgtaat gttatatcac gaattggaag tcgcagcctc
      721 agctgacgaa gtatggtctg tcgaaggttc tcctgaatta ggtttacatt taccagattt
      781 gttaccagct ggaatattcg caaagtttga gataactgga gatggtggag aaggttctat
      841 tttggacatg acatttccac ctggtcaatt ccctcatcat tatagagaga agttcgtttt
      901 cttcgaccat aagaatagat ataagttggt tgaacaaatt gacggtgatt tctttgattt
      961 gggagtaacc tactacatgg acactattag ggtcgtcgca acaggtcctg attcttgtgt
     1021 catcaaatca acaactgaat atcatgttaa accagaattt gctaagattg ttaaaccttt
     1081 aatcgataca gttccattag ccattatgtc agaggcaata gctaaggtag tattggaaaa
     1141 taagcacaaa tcttctgagg gatccgggtc ctctaaaggt gaagaattat tcactggtgt
     1201 tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg
     1261 tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaattgattt gtactactgg
     1321 taaattgcca gttccatggc caaccttagt cactacttta ggttatggtt tgcaatgttt
     1381 tgctagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg
     1441 ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga
     1501 agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa
     1561 agaaggtggt aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta
     1621 catcactgct gacaaacaaa agaatggtat caaagctaac ttcaaaatta gacacaacat
     1681 tgaagatggt ggtgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg
     1741 tccagtcttg ttaccagaca accattactt atcctatcaa tctgccttat ccaaagatcc
     1801 aaacgaaaag agagatcaca tggtcttgtt agaatttgtt actgctgctg gtattaccca
     1861 tggtatggat gaattgtaca aagggtcctt gggaagaggt agaagatcca aattgtaata
     1921 atggcagtgc ttttaactaa gaattattag tcttttctgc ttattttttc atcatagttt
     1981 agaacacttt atattaacga atagtttatg aatctattta ggtttaaaaa ttgatacagt
     2041 tttataagtt actttttcaa agactcgtgc tgtctattgc ataatgcact ggaaggggaa
     2101 aaaaaaggtg cacacgcgtg gctttttctt gaatttgcag tttgaaaaat gctggaaatc
     2161 tgctcgtcag tggtgctcac actgacgaat catgtacaga tcataccgat gactgcctgg
     2221 cgactcacaa ctaagcaaga cagccggaac cagcgccggc gaacaccact gcatatatgg
     2281 catatcacaa cagtccacgt ctcaagcagt tacagagatg ttacgaacca ctagtgcact
     2341 gcagtacatc gaggagaact tctagtatat ctacatacct aatattattg ccttattaaa
     2401 aatggaatcc caacaattac atcaaaatcc acattctctt caaaatcaat tgtcctgtac
     2461 ttccttgttc atgtgtgttc aaaaacgtta tatttatagg ataattatac tctatttctc
     2521 aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga
     2581 ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
     2641 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
     2701 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
     2761 caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag
     2821 aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat
     2881 ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc
     2941 ttacctttta catttcagca atatatatat atatatttca aggatatacc attctaatgt
     3001 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
     3061 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
     3121 aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg
     3181 cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat
     3241 ggggaaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
     3301 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
     3361 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
     3421 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
     3481 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
     3541 agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat
     3601 ggagaaaaac tgtggaggaa accatcaaga acgaatttcc tacattgaag gttcaacatc
     3661 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
     3721 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
     3781 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
     3841 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta
     3901 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
     3961 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
     4021 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
     4081 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
     4141 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
     4201 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
     4261 ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat
     4321 taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa
     4381 ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat
     4441 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat
     4501 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac
     4561 gtagtcgaga gtaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
     4621 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     4681 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
     4741 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     4801 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
     4861 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
     4921 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
     4981 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
     5041 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
     5101 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
     5161 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
     5221 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
     5281 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
     5341 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
     5401 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
     5461 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcatcagag cgcttttggt
     5521 tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac ttcccgagcg
     5581 gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     5641 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     5701 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     5761 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     5821 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     5881 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     5941 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg
     6001 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     6061 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     6121 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     6181 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     6241 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     6301 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     6361 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
     6421 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     6481 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     6541 tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg
     6601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7261 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7321 ctgattctgt ggataaccgt gcggccgccc ctgaattcgc atctagactg atgagacgtg
     7381 gtagagccac aaacagccgg tacaagcaac gatctccagg accatctgaa tcatgcgcgg
     7441 atgacacgaa ctcacgacgg cgatcacaga cattaaccca cagtacagac actgcgacaa
     7501 cgtggcaatt cgtcgcaata caacgcagtt cgagttta
//
