LOCUS       sequence-311878-        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of truncated (delta1-1088) ER isoform 
            of rat USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  TITLE     rat USP19 constructs
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HisFlag-rUSP19ER deltaN2
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        132..511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        512..715
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     666..686
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     760..779
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        760..778
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      885..894
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             897..914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             915..980
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4238..4255)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4244..4468
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4514..4942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4601..4620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4811..4832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4951..4971)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4956..5285
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5136..5271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5352..6146
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5406..5425)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6016..6035
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6322..6443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6359..6378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6413..6432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6492..6508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6492..6508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6505..6527)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6516..6532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6540..6546,6547..6564,6565..6570))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6585..6606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6723..6740)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6894..7482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6974..6993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7653..8444,8445..8513))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8276..8295
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8514..8618)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8693..8712)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
       61 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      121 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      181 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      241 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      301 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      361 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      421 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      481 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      541 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      601 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      661 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      721 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      781 gacccaagct ggctagttaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg
      841 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagccacc atggcacatc
      901 atcatcatca tcacgactac aaagaccatg acggtgatta taaagatcat gacatcgatt
      961 acaaggatga cgatgacaag cgcccgggct gcaggaattc ccagaccagg gatggaaatt
     1021 ttctgaggct gcatccgggc tgtgggcccc acaccatctt ccgctggcag gtgaagctca
     1081 ggaacttgat tgagccagag caatgtacgt tttgtttcac ggcctctcga atcgatatct
     1141 gcctccggaa gcggcagagt cagcgctggg ggggactgga ggcccctgct acacgagtgg
     1201 gtggtgcaaa ggttgccgtg ccgacaggtc caaccccttt ggattcaacc cctccaggag
     1261 gtggccccct ccctctgact ggccaggagg aagccagggc tgtagagaag gaaaaaccca
     1321 aggctcggtc agaggactcg gggctggatg gtgtggtggc tcgcaccccc ttggagcatg
     1381 ttaccccaaa gccagaaccg cacttggcct cgcccaaacc cacatgtatg gtgcctccaa
     1441 tgcctcatag tccagtgagt ggagatagtg tggaggagga tgaagaggaa gagaagaagg
     1501 tttgtctccc aggctttact ggcctcgtca acttagggaa cacctgcttc atgaatagtg
     1561 tcattcagtc tctgtccaac actcgggagc ttcgtgactt cttccacgac cgatcctttg
     1621 aggctgagat taactacaat aacccattgg ggactggtgg gcgtctggct attggcttcg
     1681 ctgtgctgct ccgggccctg tggaagggca ctcaccaagc ctttcaaccc tccaagctaa
     1741 aggccattgt agcaagcaag gccagccagt ttacaggcta cgcacagcat gatgcccaag
     1801 agttcatggc tttcttgttg gatgggctgc atgaagacct gaatcggatc caaaacaaac
     1861 cctacacaga gactgtggac tcagatgggc gacctgacga ggtggtggct gaagaagcat
     1921 ggcagcggca caagatgagg aacgactcat tcattgtgga cctgtttcag ggccagtaca
     1981 agtcaaagct ggtgtgccct gtgtgtgcca aggtctccat cacttttgac ccgttccttt
     2041 atctgccggt acccttgcca caaaagcaaa aggttctccc catatattat tttgccagag
     2101 agccccatag caaacccatc aagttcctgg tgagcgtcag caaggagaac tccagcgcaa
     2161 gcgaagtttt ggagtccctc tctcagagtg tccacgtgaa gcctgagagc ctgcgcctgg
     2221 ctgaggtaat taagaatcgt ttccaccgtg ttttcttgcc ctcccactca ctggatgctg
     2281 tgtcgccaac ggacgtgctc ctgtgctttg agctgctatc cccagaattg gctaaggaga
     2341 gggtagtggt gctagaggtg cagcagcgcc cccaggtacc cagcatccct atctccaagt
     2401 gtgcagcctg ccagcggaag cagcaatcag aagatgaaaa actgaagcgt tgtacccgtt
     2461 gctaccgtgt gggctactgc aaccagttct gtcagaaaac ccattggcct gaccacaaag
     2521 gcctctgccg ccccgagaac attggctacc ccttcctggt cagtgtacct gcttcacgcc
     2581 tcacttatgc ccgtcttgct cagctactag aaggttatgc ccggtactct gtgagcgtat
     2641 tccaaccacc cttccagcct ggccgaatgg ctttggaatc acagagccct ggctgtacca
     2701 cgttgctttc aaccagctct ctggaggctg gggatagtga aagagaaccc attcagcctt
     2761 ctgagctcca gttggtgact cctgtggctg agggggatac cggggctcac cgcatgtggc
     2821 cacctgctga tcggggtcct gtgcctagca ccagtggaat ttcttccgag atgctggcca
     2881 gtgggcctat ggaagggtgt tccttgcttg ctggtgagag ggtgtctcgg cctgaagctg
     2941 ctgtgcctgg gtaccagcac tcacgtgagt ctgtgagcgc ccacacgccc cagttcttca
     3001 tctataaaat tgatgcgtca agccgtgagc agcggctgga ggacaaaggg gacactccac
     3061 tggagctagg tgatgactgt agcctggctc tggtgtggcg gaacaacgaa cgccttcagg
     3121 agtttgtgct ggtggcctcc aaggagctgg agtgtgctga agacccaggc tccgctggtg
     3181 aggctgcccg tgctggccac ttcaccctgg accagtgcct caatctcttt acacggcctg
     3241 aagtgctggc acctgaggaa gcctggtact gcccacagtg caaacagcat cgagaggcct
     3301 ccaagcagct gctgctgtgg cgcctaccaa atgtgctcat cgtgcagctc aagcgtttct
     3361 cctttcgtag cttcatttgg cgtgacaaga tcaatgactt ggtggagttt ccagttcgga
     3421 acctggactt gagcaagttc tgtattggtc agaaagagga gcagctgcct agctatgacc
     3481 tgtatgcagt catcaaccac tacggaggca tgatcggcgg ccactacact gcctgtgcac
     3541 ggctgcccag tgaccgtagt agccagcgca gtgacgtggg ctggcgcttg tttgatgaca
     3601 gcacggtgac aacagtagac gagagccagg tggtgacacg ctatgcctat gtgctcttct
     3661 accgtcggcg gaactctcct gtggagaggc cccccagggc agctcacgct gaacaccacc
     3721 cagacctagg ccctgcagcc gaggctgctg ccagccaggc ttcccggatt tggcaggagc
     3781 tcgaggccga ggaggagatg gtgcccgagg ggcctgggcc tctgggtcct tgggggcccc
     3841 aagactgggt ggggcccccg ccacgtggcc ctaccacatc agacgagggc tgcctccgat
     3901 actttgtcct gggtaccgtg gcggctttgg tggcccttgt gctcaacgta ttctatcctc
     3961 tggtatctca gagtcgctgg agatgagctc ccctgcaggc gcctgctgtg agctggccta
     4021 cctatgctca ccaggacagg cctgcctttg tggtgggaga cagctctggg ctctgagcct
     4081 cagtgtatgt atctggtggg agacttgttg ggactacagc ctctgtgggg gcagagtatc
     4141 ctagggtgtg tgtccatcca gttgtctgtc ctcctgtttc tctgaccctt gtcctagggc
     4201 ttggggaatt cgatatcaag ctaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4261 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4321 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4381 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4441 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4501 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4561 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4621 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4681 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4741 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4801 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4861 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4921 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4981 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5041 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5101 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5161 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5221 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5281 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5341 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5401 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5461 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5521 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5581 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5641 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5701 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5761 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5821 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5881 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5941 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6001 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6061 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6121 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
     6181 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6241 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6301 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6361 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6421 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6481 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6541 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6661 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7561 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7621 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7681 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7741 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7801 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7861 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7921 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7981 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8041 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8101 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8161 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8221 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8281 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8341 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8401 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8461 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8521 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8581 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8641 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     8701 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg ga
//
