LOCUS       sequence-311879-        6283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UbiY-Venus-ePTS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
  TITLE     Peroxisome compartmentalization of a toxic enzyme improves alkaloid 
            production.
  JOURNAL   Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4.
  PUBMED    33046851
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4."
COMMENT     Sequence Label: pJJB207
FEATURES             Location/Qualifiers
     source          1..6283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             674..901
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             1028..1738
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2389..3192
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(3834..4649)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3834..4649)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4557..4576
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4641..4660
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4874..5462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5363..5382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctatgcaga ttttcgttaa aactttaact ggtaaaacaa ttactttaga
      721 agttgagtca tctgatacta tcgataatgt taaatctaag atccaggata aggaaggtat
      781 tcctccagat caacaaagat tgattttcgc aggtaagcag ttggaggacg gtaggacttt
      841 gtctgattat aacatacaga aagaatctac tttgcactta gtcttaagat taaggggagg
      901 ttatcatggt tctggtgcct ggttgttacc tgtatcattg gttaaaagaa agaccacatt
      961 ggcaccaaat actcaaactg cctctccaag agccttggca gactctttaa tgcagagatc
     1021 aggttcttct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     1081 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
     1141 cggtaaattg accttaaaat tgatttgtac tactggtaaa ttgccagttc catggccaac
     1201 cttagtcact actttaggtt atggtttgca atgttttgct agatacccag atcatatgaa
     1261 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     1321 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     1381 agttaataga atcgaattaa aaggtattga ttttaaagaa ggtggtaaca ttttaggtca
     1441 caaattggaa tacaactata actctcacaa tgtttacatc actgctgaca aacaaaagaa
     1501 tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg ttcaattagc
     1561 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     1621 ttacttatcc tatcaatctg ccttatccaa agatccaaac gaaaagagag atcacatggt
     1681 cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaagg
     1741 gtccttggga agaggtagaa gatccaaatt gtaaggatcc taactcgaga taaagcaatc
     1801 ttgatgagga taatgatttt tttttgaata tacataaata ctaccgtttt tctgctagat
     1861 tttgtgatga cgtaaataag tacatattac tttttaagcc aagacaagat taagcattaa
     1921 ctttaccctt ttctttctaa gtttcaatat tagttatcac tgtttaaaag ttatggcgag
     1981 aacgtcggcg gttaaaatat attaccctga acggctggaa atctgctcgt cagtggtgct
     2041 cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca caactaagca
     2101 agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca caacagtcca
     2161 cgtctcaagc agttacagag atgttacgaa ccactagtgc actgcagtac acggtttcct
     2221 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     2281 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     2341 ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct
     2401 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     2461 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     2521 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     2581 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     2641 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
     2701 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     2761 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     2821 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     2881 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     2941 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     3001 ggtttagatg acaagggaga tgcattgggt caacagtata gaaccgtgga tgatgtggtt
     3061 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     3121 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     3181 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     3241 gcttcaattt aattatatca gttattaccc gagtagagca cttgaatcca ctgccccggg
     3301 aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
     3361 catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa cagtatttta
     3421 cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcatt gaactccaaa
     3481 gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt catgcgtttg
     3541 tactctaatc tggtagaaag atgtttcaca gactgtgtca atgacttcac aacatcaaag
     3601 ctaaccaata aggaacaaac atgcatcatg aagtgctcag aaaagttctt gaagcatagc
     3661 gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg cttgggccga
     3721 taaggtgtac tggcgtatat atatctaatt atgtatctct ggtgtagccc atttttagca
     3781 tgtaaatata aagaccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa
     3841 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     3901 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     3961 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     4021 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     4081 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     4141 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     4201 gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     4261 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     4321 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     4381 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     4441 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     4501 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     4561 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag
     4621 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     4681 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     4741 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca
     4801 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4861 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4921 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4981 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5041 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5101 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5161 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5221 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5281 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5341 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5401 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5461 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5521 tgttctttcc tgcgttatcc cctgattctg tggataaccg tgcggccgcc aatgctaaat
     5581 tcgagtgaaa cacaggaaga tcagaaaatc ctcatttcat ccatattaac aataatttca
     5641 aatgtttatt tgcattattt gaaactaggc aagacaagca acgaaacgtt tttgaaaatt
     5701 ttgagtattt tcaataaatt tgtagaggac tcagatattg aaaaaaagct acagcaatta
     5761 atacttgata agaagagtat tgagaagggc aacggttcat catctcatgg atctgcacat
     5821 gaacaaacac cagagtcaaa cgacgttgaa attgaggcta ctgcgccaat tgatgacaat
     5881 acagacgatg ataacaaacc gaagttatct gatgtagaaa aggattaaag atgctaagag
     5941 atagtgatga tatttcataa ataatgtaat tctatatatg ttaattacct tttttgcgag
     6001 gcatatttat ggtgaaggat aagttttgac catcaaagaa ggttaatgtg gctgtggttt
     6061 cagggtccat aaagcccaca tggataacat tacccctgaa ttcgcatcta gactgatgag
     6121 acgtggtaga gccacaaaca gccggtacaa gcaacgatct ccaggaccat ctgaatcatg
     6181 cgcggatgac acgaactcac gacggcgatc acagacatta acccacagta cagacactgc
     6241 gacaacgtgg caattcgtcg caatacaacg cagttcgagt tta
//
