LOCUS       sequence-31188-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-UEV1A
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(581..599)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      595..642
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(817..822,823..839,840..845))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(872..890)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaatatttt gtttacttta gaaggagata taccatgcat catcatcatc atcacagcag
       61 cggcagagaa aacttgtatt tccagggcat tgttccgcgt tcttttcgcc tgttagatga
      121 gttggagcgg ggccagaaag gtaatgtcag tgaaggggta tcattcggac ttgaatccgc
      181 ggacgatatc accctctcga actggagctg cacgatattt ggccaaccag gtactgtgtt
      241 cgaaaataga atttattctc tgacaatctt ttgtgacgat aactacccgg acagtcctcc
      301 caccgttaag ttcgatacga aaattgagat gtcatgcgtc gataattgcg ggcgagtaat
      361 caaaaacaac ctgcatatcc tgaagaactg gaaccgtaac tacaccatcg agacgatcct
      421 gatttccctg cgccaggaaa tgttatcgag cgccaataaa cgtttgccac aacctaatga
      481 gggagaagtg tattccaata actgatgacg aagcttgcgg ccgcactcga ggatccggct
      541 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      601 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      661 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
      721 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
      781 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
      841 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      901 aatgtcatga taataatggt ttcttagacg tcaggtggca c
//
