LOCUS       sequence-311880-        8209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of truncated (delta1-1631) ER isoform 
            of rat USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8209)
  TITLE     rat USP19 constructs
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HisFlag-rUSP19ER deltaN3
FEATURES             Location/Qualifiers
     source          1..8209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        91..470
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        471..674
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     625..645
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     719..738
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        719..737
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      844..853
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             856..873
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             874..939
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(3654..3671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3660..3884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3930..4358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4017..4036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4227..4248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4367..4387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4372..4701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4552..4687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4768..5562
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4822..4841)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5432..5451
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5738..5859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5775..5794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5829..5848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5908..5924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5908..5924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5921..5943)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5932..5948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5956..5962,5963..5980,5981..5986))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6001..6022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6139..6156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6310..6898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6390..6409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7069..7860,7861..7929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7692..7711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7930..8034)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8109..8128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
       61 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      661 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
      721 atacgactca ctatagggag acccaagctg gctagttaag cttggtaccg agctcggatc
      781 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
      841 agagccacca tggcacatca tcatcatcat cacgactaca aagaccatga cggtgattat
      901 aaagatcatg acatcgatta caaggatgac gatgacaagc gcccgggctg caggaattcc
      961 tgcttcatga atagtgtcat tcagtctctg tccaacactc gggagcttcg tgacttcttc
     1021 cacgaccgat cctttgaggc tgagattaac tacaataacc cattggggac tggtgggcgt
     1081 ctggctattg gcttcgctgt gctgctccgg gccctgtgga agggcactca ccaagccttt
     1141 caaccctcca agctaaaggc cattgtagca agcaaggcca gccagtttac aggctacgca
     1201 cagcatgatg cccaagagtt catggctttc ttgttggatg ggctgcatga agacctgaat
     1261 cggatccaaa acaaacccta cacagagact gtggactcag atgggcgacc tgacgaggtg
     1321 gtggctgaag aagcatggca gcggcacaag atgaggaacg actcattcat tgtggacctg
     1381 tttcagggcc agtacaagtc aaagctggtg tgccctgtgt gtgccaaggt ctccatcact
     1441 tttgacccgt tcctttatct gccggtaccc ttgccacaaa agcaaaaggt tctccccata
     1501 tattattttg ccagagagcc ccatagcaaa cccatcaagt tcctggtgag cgtcagcaag
     1561 gagaactcca gcgcaagcga agttttggag tccctctctc agagtgtcca cgtgaagcct
     1621 gagagcctgc gcctggctga ggtaattaag aatcgtttcc accgtgtttt cttgccctcc
     1681 cactcactgg atgctgtgtc gccaacggac gtgctcctgt gctttgagct gctatcccca
     1741 gaattggcta aggagagggt agtggtgcta gaggtgcagc agcgccccca ggtacccagc
     1801 atccctatct ccaagtgtgc agcctgccag cggaagcagc aatcagaaga tgaaaaactg
     1861 aagcgttgta cccgttgcta ccgtgtgggc tactgcaacc agttctgtca gaaaacccat
     1921 tggcctgacc acaaaggcct ctgccgcccc gagaacattg gctacccctt cctggtcagt
     1981 gtacctgctt cacgcctcac ttatgcccgt cttgctcagc tactagaagg ttatgcccgg
     2041 tactctgtga gcgtattcca accacccttc cagcctggcc gaatggcttt ggaatcacag
     2101 agccctggct gtaccacgtt gctttcaacc agctctctgg aggctgggga tagtgaaaga
     2161 gaacccattc agccttctga gctccagttg gtgactcctg tggctgaggg ggataccggg
     2221 gctcaccgca tgtggccacc tgctgatcgg ggtcctgtgc ctagcaccag tggaatttct
     2281 tccgagatgc tggccagtgg gcctatggaa gggtgttcct tgcttgctgg tgagagggtg
     2341 tctcggcctg aagctgctgt gcctgggtac cagcactcac gtgagtctgt gagcgcccac
     2401 acgccccagt tcttcatcta taaaattgat gcgtcaagcc gtgagcagcg gctggaggac
     2461 aaaggggaca ctccactgga gctaggtgat gactgtagcc tggctctggt gtggcggaac
     2521 aacgaacgcc ttcaggagtt tgtgctggtg gcctccaagg agctggagtg tgctgaagac
     2581 ccaggctccg ctggtgaggc tgcccgtgct ggccacttca ccctggacca gtgcctcaat
     2641 ctctttacac ggcctgaagt gctggcacct gaggaagcct ggtactgccc acagtgcaaa
     2701 cagcatcgag aggcctccaa gcagctgctg ctgtggcgcc taccaaatgt gctcatcgtg
     2761 cagctcaagc gtttctcctt tcgtagcttc atttggcgtg acaagatcaa tgacttggtg
     2821 gagtttccag ttcggaacct ggacttgagc aagttctgta ttggtcagaa agaggagcag
     2881 ctgcctagct atgacctgta tgcagtcatc aaccactacg gaggcatgat cggcggccac
     2941 tacactgcct gtgcacggct gcccagtgac cgtagtagcc agcgcagtga cgtgggctgg
     3001 cgcttgtttg atgacagcac ggtgacaaca gtagacgaga gccaggtggt gacacgctat
     3061 gcctatgtgc tcttctaccg tcggcggaac tctcctgtgg agaggccccc cagggcagct
     3121 cacgctgaac accacccaga cctaggccct gcagccgagg ctgctgccag ccaggcttcc
     3181 cggatttggc aggagctcga ggccgaggag gagatggtgc ccgaggggcc tgggcctctg
     3241 ggtccttggg ggccccaaga ctgggtgggg cccccgccac gtggccctac cacatcagac
     3301 gagggctgcc tccgatactt tgtcctgggt accgtggcgg ctttggtggc ccttgtgctc
     3361 aacgtattct atcctctggt atctcagagt cgctggagat gagctcccct gcaggcgcct
     3421 gctgtgagct ggcctaccta tgctcaccag gacaggcctg cctttgtggt gggagacagc
     3481 tctgggctct gagcctcagt gtatgtatct ggtgggagac ttgttgggac tacagcctct
     3541 gtgggggcag agtatcctag ggtgtgtgtc catccagttg tctgtcctcc tgtttctctg
     3601 acccttgtcc tagggcttgg ggaattagat atcaagctaa acccgctgat cagcctcgac
     3661 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3721 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3781 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3841 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3901 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4381 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4441 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4501 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4561 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4621 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4681 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4741 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4921 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5281 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5581 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5641 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5701 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5761 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5821 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5881 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5941 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6001 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6061 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6121 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8041 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8101 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8161 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaa
//
