LOCUS       sequence-311883-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A
  TITLE     An N-end rule pathway that recognizes proline and destroys 
            gluconeogenic enzymes.
  JOURNAL   Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 
            10.1126/science.aal3655.
  PUBMED    28126757
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-01-27"; volume: "355(6323). pii: 355/6323/eaal3655. 
            doi"; pages: " 10.1126/science.aal3655"
COMMENT     Sequence Label: pCSJ182
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1174..1878
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1924..1944
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1963..1987
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3082..3147
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3149..3173)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3187..3528
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      3973..4160
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4028..4052)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4277..5371)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5372..5777)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5819..5835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5819..5835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5832..5854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5843..5859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5867..5873,5874..5891,5892..5897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5912..5933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5988..6021
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6099..6132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6201..6218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6372..6960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6452..6471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7131..7922,7923..7991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7754..7773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7992..8096)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8164..8182
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8220..8242)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8342..8361
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      join(8378..9142,1..400)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg
       61 agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg
      121 tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc
      181 tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat
      241 ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc
      301 atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
      361 tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt gcttgcatgc ctgcaggtcg
      421 agatccggga tcgaagaaat gatggtaaat gaaataggaa atcaaggagc atgaaggcaa
      481 aagacaaata taagggtcga acgaaaaata aagtgaaaag tgttgatatg atgtatttgg
      541 ctttgcggcg ccgaaaaaac gagtttacgc aattgcacaa tcatgctgac tctgtggcgg
      601 acccgcgctc ttgccggccc ggcgataacg ctgggcgtga ggctgtgccc ggcggagttt
      661 tttgcgcctg cattttccaa ggtttaccct gcgctaaggg gcgagattgg agaagcaata
      721 agaatgccgg ttggggttgc gatgatgacg accacgacaa ctggtgtcat tatttaagtt
      781 gccgaaagaa cctgagtgca tttgcaacat gagtatacta gaagaatgag ccaagacttg
      841 cgagacgcga gtttgccggt ggtgcgaaca atagagcgac catgaccttg aaggtgagac
      901 gcgcataacc gctagagtac tttgaagagg aaacagcaat agggttgcta ccagtataaa
      961 tagacaggta catacaacac tggaaatggt tgtctgtttg agtacgcttt caattcattt
     1021 gggtgtgcac tttattatgt tacaatatgg aagggaactt tacacttctc ctatgcacat
     1081 atattaatta aagtccaatg ctagtagaga aggggggtaa cacccctccg cgctcttttc
     1141 cgattttttt ctaaaccgtg gaatatttcg gatatccttt tgttgtttcc gggtgtacaa
     1201 tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg
     1261 ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca
     1321 agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata
     1381 acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc
     1441 tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt
     1501 ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaatgatgga
     1561 agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt tgttccagag
     1621 ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca cgcacactac
     1681 tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga aataaaaaaa
     1741 agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc
     1801 gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata
     1861 ccaagcatac aatcaactcc aagctttgca aagatggata aagcggaatt aattcccgag
     1921 cctccaaaaa agaagagaaa ggtcgaattg ggtaccgcat caacaagttt gtacaaaaaa
     1981 gcaggcttca tgatcaataa tcctaaggta gacagtgtag cggagaaacc caaagctgtg
     2041 acatcaaagc agtcggagca agcggcttcg ccagaaccaa caccagcccc ccctgtttct
     2101 agaaatcagt atccgatcac gttcaacttg acttcaaccg caccctttca tcttcatgac
     2161 cgtcatcgct acttacaaga gcaagatctt tacaagtgcg cttctaggga ttcgttgtct
     2221 tccctgcagc aactcgccca tacacctaat gggtcgacaa ggaagaaata tattgttgag
     2281 gaccaatctc cctatagttc ggaaaatcca gtcattgtga cctcttccta taaccatacg
     2341 gtttgcacaa actacttaag accaagaatg cagtttacag ggtaccagat atcaggatac
     2401 aaacgctatc aggtaacagt taacttaaag actgtagatt tgccaaagaa agattgcacg
     2461 tcgctgtccc ctcatttatc tggatttttg tcgataagag gactcacgaa ccaacacccg
     2521 gaaatcagta catattttga agcctacgcg gtaaaccaca aggaattagg gttcttgtcc
     2581 tctagctgga aagatgaacc tgttttaaac gaattcaaag ccacagacca aacagactta
     2641 gaacactgga taaatttccc ctcctttaga cagcttttcc tgatgagcca aaaaaacggt
     2701 ctcaactcaa ctgacgacaa tggcactaca aatgcagcca agaagttgcc tccacagcag
     2761 cttcctacta caccaagcgc agacgctggt aacatatcaa gaatttttag ccaagagaaa
     2821 caatttgaca actacttgaa cgaacggttt atatttatga aatggaagga aaaatttttg
     2881 gtaccagatg ccctattaat ggaaggtgta gacggcgcat cttatgatgg gttttattac
     2941 attgtccatg atcaagttac cgggaacatt caagggtttt actatcatca agatgctgaa
     3001 aagttccaac agctggaatt agtaccatct ttgaaaaata aagtcgagtc cagtgattgt
     3061 tcttttgagt ttgctcccgg ggattacaag gatcatgatg gtgattacaa ggatcatgac
     3121 attgactata aggatgatga tgacaaagac ccagctttct tgtacaaagt ggttgatggt
     3181 accgccgcca attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc
     3241 actaacagta gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca
     3301 caaccaattg cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt
     3361 aaaattgatg atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg
     3421 tataacgcgt ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat
     3481 aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagat cctcgagtac
     3541 gacgtaccag attacgctta gtgatcacga tacgggatcc atcgagctcg agctgcagat
     3601 gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc
     3661 aatttctttc atttatacat cgttttgcct tcttttatgt aactatactc ctctaagttt
     3721 caatcttggc catgtaacct ctgatctata gaatttttta aatgactaga attaatgccc
     3781 atcttttttt tggacctaaa ttcttcatga aaatatatta cgagggctta ttcagaagct
     3841 ttggacttct tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc
     3901 ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac
     3961 acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     4021 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     4081 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt
     4141 attgaccaca cctctaccgg ccggtcgaaa ttcccctacc ctatgaacat attccatttt
     4201 gtaatttcgt gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata
     4261 aaaaaagaga atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga
     4321 cttcggtggt actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct
     4381 ttttaactgc atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca
     4441 tcattgcagc agacaagata gtggcgatag ggttgacctt attctttggc aaatctggag
     4501 cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca
     4561 aggacgcaga tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga
     4621 tatcaccaaa catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta
     4681 ggatcatggc ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct
     4741 tgatggtttc ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat
     4801 ccaaggacca aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg
     4861 tgattctttg cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat
     4921 cgtcttcctt tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt
     4981 cagtaccttt agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa
     5041 agttacatgg tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt
     5101 gttcaggtct aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg
     5161 catcagcctt cttggaggct tccagcgcct catctggaag tgggacacct gtagcttcga
     5221 tagcagcacc accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag
     5281 aaatagcttt aagaacctta atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg
     5341 gcaaaacgac gatcttctta ggggcagaca ttagaatggt atatccttga aatatatata
     5401 tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg ctaaccacct
     5461 attggaaaaa acaataggtc cttaaataat attgtcaact tcaagtattg tgatgcaagc
     5521 atttagtcat gaacgcttct ctattctata tgaaaagccg gttccggcgc tctcaccttt
     5581 cctttttctc ccaatttttc agttgaaaaa ggtatatgcg tcaggcgacc tctgaaatta
     5641 acaaaaaatt tccagtcatc gaatttgatt ctgtgcgata gcgcccctgt gtgttctcgt
     5701 tatgttgagg aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc ttacgatacc
     5761 tgagtattcc cacagttggg ggatctcgac tctagctaga ggatcaattc gtaatcatgt
     5821 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5881 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5941 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgata acttcgtata
     6001 atgtatgcta tacgaagtta ttaggtctga agaggagttt acgtccagcc aagctagctt
     6061 ggctgcaggt cgagcggccg cgatccggaa cccttaatat aacttcgtat aatgtatgct
     6121 atacgaagtt atcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6181 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6241 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6361 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6541 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6661 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6721 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6781 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6841 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6901 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6961 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7021 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7081 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7141 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7201 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7261 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7321 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7381 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7441 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7501 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7561 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7621 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7681 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7741 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7801 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7861 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7921 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7981 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8041 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8101 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8161 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     8221 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     8281 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     8341 gcggcatcag agcagattgt actgagagtg caccataacg catttaagca taaacacgca
     8401 ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt
     8461 gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa
     8521 acgctttgaa gttcctattc cgaagttcct attctctagc tagaaagtat aggaacttca
     8581 gagcgctttt gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc aaaaacgcac
     8641 cggactgtaa cgagctacta aaatattgcg aataccgctt ccacaaacat tgctcaaaag
     8701 tatctctttg ctatatatct ctgtgctata tccctatata acctacccat ccacctttcg
     8761 ctccttgaac ttgcatctaa actcgacctc tacatttttt atgtttatct ctagtattac
     8821 tctttagaca aaaaaattgt agtaagaact attcatagag tgaatcgaaa acaatacgaa
     8881 aatgtaaaca tttcctatac gtagtatata gagacaaaat agaagaaacc gttcataatt
     8941 ttctgaccaa tgaagaatca tcaacgctat cactttctgt tcacaaagta tgcgcaatcc
     9001 acatcggtat agaatataat cggggatgcc tttatcttga aaaaatgcac ccgcagcttc
     9061 gctagtaatc agtaaacgcg ggaagtggag tcaggctttt tttatggaag agaaaataga
     9121 caccaaagta gccttcttct aa
//
