LOCUS       sequence-311892-        1242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express a fragment of mouse Pola1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL,
            Xu RM, Zhang Z
  TITLE     DNA polymerase alpha interacts with H3-H4 and facilitates the 
            transfer of parental histones to lagging strands.
  JOURNAL   Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. 
            eCollection 2020 Aug.
  PUBMED    32923642
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-08-26"; volume: "6(35):eabb5820. doi"; pages: " 
            10.1126/sciadv.abb5820. eCollection 2020 Aug"
COMMENT     Sequence Label: pGST-POLA1-N1
FEATURES             Location/Qualifiers
     source          1..1242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(535..557)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(702..720)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gap             883..895
                     /estimated_length=13
     gap             919..930
                     /estimated_length=12
     gap             951..964
                     /estimated_length=14
     gap             978..994
                     /estimated_length=17
     gap             1013..1023
                     /estimated_length=11
     gap             1025..1037
                     /estimated_length=13
     gap             1039..1062
                     /estimated_length=24
     gap             1073..1084
                     /estimated_length=12
     gap             1094..1121
                     /estimated_length=28
     gap             1125..1139
                     /estimated_length=15
     gap             1141..1160
                     /estimated_length=20
     gap             1172..1197
                     /estimated_length=26
     gap             1200..1209
                     /estimated_length=10
     gap             1212..1227
                     /estimated_length=16
     gap             1229..1239
                     /estimated_length=11
ORIGIN
        1 tnggtggtgg cgaccntcct cccaaaatcg atacgaccga aaacctgtat tttcagggcg
       61 ccatgatggc gcccatgcac gaagaggact gtaaactgga ggcaagcgct gtgtcagatt
      121 cagggagttt tgcagcttcc cgagctcggc gagaaaagaa atcaaagaaa ggacgtcaag
      181 aagctttaga gagactgaaa aaggctaaag ctggggagaa gtataaatac gaagttgagg
      241 acctcacaag tgtttatgaa gaagttgatg aagagcagta ttcgaaactg gttcaggccc
      301 gtcaggatga tgactggatt gtggatgacg atgggatagg ctatgtggaa gatggcagag
      361 agatttttga tgatgacctt gaggatgatg cactttgaga tccggaattc aaaggcctac
      421 gtcgacgagc tcaactagtg cggccgcttt cgaatctaga gcctgcagtc tcgagcggcc
      481 gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
      541 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      601 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
      661 acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga tacgcctatt
      721 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      841 catgagacaa taaccctgan aantgcctca ataatattga aannnnnnnn nnnnnantan
      901 tncnncnntn nccnntngnn nnnnnnnnnn ttnngnnnna nntnnnntnc nnnnnnnnnn
      961 nnnntccnnn annnnngnnn nnnnnnnnnn nnnnccnncn nanntnnntg ncnnnnnnnn
     1021 nnntnnnnnn nnnnnnngnn nnnnnnnnnn nnnnnnnnnn nncnntnnnn ntnnnnnnnn
     1081 nnnnannnnn nntnnnnnnn nnnnnnnnnn nnnnnnnnnn ncntnnnnnn nnnnnnnnng
     1141 nnnnnnnnnn nnnnnnnnnn ccncnnnnnn tnnnnnnnnn nnnnnnnnnn nnnnnnntcn
     1201 nnnnnnnnnc cnnnnnnnnn nnnnnnntnn nnnnnnnnnc cc
//
