LOCUS       sequence-311893-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express a fragment of mouse Pola1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL,
            Xu RM, Zhang Z
  TITLE     DNA polymerase alpha interacts with H3-H4 and facilitates the 
            transfer of parental histones to lagging strands.
  JOURNAL   Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. 
            eCollection 2020 Aug.
  PUBMED    32923642
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-08-26"; volume: "6(35):eabb5820. doi"; pages: " 
            10.1126/sciadv.abb5820. eCollection 2020 Aug"
COMMENT     Sequence Label: pGST-POLA1-N2
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..12
                     /estimated_length=11
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(823..845)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cnnnnnnnnn nnanncgttt ggtggtggcg accatcctcc aaaaatcgat acgaccgaaa
       61 acctgtattt tcagggcgcc atgatggcgc ccatgcacga agaggactgt aaactggagg
      121 caagcgctgt gtcagattca gggagttttg cagcttcccg agctcggcga gaaaagaaat
      181 caaagaaagg acgtcaagaa gctttagaga gactgaaaaa ggctaaagct ggggagaagt
      241 ataaatacga agttgaggac ctcacaagtg tttatgaaga agttgatgaa gagcagtatt
      301 cgaaactggt tcaggcccgt caggatgatg actggattgt ggatgacgat gggataggct
      361 atgtggaaga tggcagagag atttttgatg atgaccttga ggatgatgca cttgacacct
      421 gtggaaaagg aagtgatggc aaagcacaca gaaaggacag gaaggatgta aagaaacctt
      481 cagtgacaaa accaaacaat atcaaggcaa tgttcattgc tagtgccggg aagaaaacta
      541 cagataaagc agtagacttg tccaaggatg atctgctagg tgacattcta caggatctaa
      601 acactgagac agctcaaata actccaccac ctgtattgat accaaagaaa aaaagatcta
      661 ctggggcttt gctcaaccct ttttgagatc cggaattcaa aggcctacgt cgacgagctc
      721 aactagtgcg gccgctttcg aatctagagc ctgcagtctc gagcggccgc atcgtgactg
      781 actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
      841 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
      901 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagncatg ac
//
