LOCUS       sequence-311894-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express a fragment of mouse Pola1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL,
            Xu RM, Zhang Z
  TITLE     DNA polymerase alpha interacts with H3-H4 and facilitates the 
            transfer of parental histones to lagging strands.
  JOURNAL   Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. 
            eCollection 2020 Aug.
  PUBMED    32923642
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-08-26"; volume: "6(35):eabb5820. doi"; pages: " 
            10.1126/sciadv.abb5820. eCollection 2020 Aug"
COMMENT     Sequence Label: pGST-POLA1-N1-2A
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..62
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(538..560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        791..895
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cnttnggngg tggcgaccnt cctccnaaaa tcgatacgac cgaaaacctg tattttcagg
       61 gcgccatgat ggcgcccatg cacgaagagg actgtaaact ggaggcaagc gctgtgtcag
      121 attcagggag ttttgcagct tcccgagctc ggcgagaaaa gaaatcaaag aaaggacgtc
      181 aagaagcttt agagagactg aaaaaggcta aagctgggga gaagtataaa tacgaagttg
      241 aggacctcac aagtgtttat gaagaagttg atgaagagca gtattcgaaa ctggttcagg
      301 cccgtcagga tgatgacgcg attgtggatg ccgatgggat aggctatgtg gaagatggca
      361 gagagatttt tgatgatgac cttgaggatg atgcactttg agatccggaa ttcaaaggcc
      421 tacgtcgacg agctcaacta gtgcggccgc tttcgaatct agagcctgca gtctcgagcg
      481 gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
      541 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
      601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
      661 gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg tgcccacgag
     1021 tgggttacat cgaactggat ctcaacagcg gnaagaa
//
